LOCUS       62056_13625        2646 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pL0R-lacZ, complete sequence.
ACCESSION   MN436816
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    complement(42..47)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    complement(50..56)
                     /label=SapI
                     /note="SapI"
     protein_bind    112..133
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        148..178
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    186..202
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     210..226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(239..295)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(296..312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    635..641
                     /label=SapI
                     /note="SapI"
     misc_feature    644..649
                     /label=BsaI
                     /note="BsaI"
     misc_feature    651..690
                     /label=UNSX
                     /note="UNSX"
     rep_origin      complement(781..1369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1463..2251)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      complement(2252..2399)
                     /label=specR promoter
                     /note="specR promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctccag aagagcgcag
       61 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      121 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      181 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      241 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgagctcg aattcactgg
      301 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      361 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      421 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
      481 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
      541 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      601 tgctcccggc atccgcttac agacaagctg tgacgctctt cacgagacca ccaggataca
      661 tagattacca caactccgag cccttccacc cacagaatca ggggataacg caggaaagaa
      721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1441 catgcattct aggtgattat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
     1501 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
     1561 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     1621 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     1681 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     1741 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1801 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1861 gatcaatgtc gatcgtggct ggctcgaaga taccagcaag aatgtcattg cgctgccatt
     1921 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     1981 caatggtgac ttctacagcg cggagaatct cactctctcc aggggaagcc gaagtttcca
     2041 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     2101 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     2161 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     2221 tcgatacttc ggcgatcacc gcttccctca tgcgaaacga tcctcatcct gtctcttgat
     2281 cagatattga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     2341 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgcta
     2401 agcttgcatg cctgcaagtc gactctagag gagcggtgat cacaggcagc aacgctctgt
     2461 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     2521 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     2581 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     2641 cgagct
//
