LOCUS       pKindling-Red-B.        4106 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing KFP-Red in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pKindling-Red-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             133..828
                     /label=KFP-Red
                     /note="photoactivatable nonfluorescent protein that becomes
                     red fluorescent upon exposure to green light"
     terminator      852..946
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             990..1646
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1714..1800
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1956..2096
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2282..2870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3044..3901)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3902..4006)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttgattaaag aggagaaatt aactatgaga
      121 ggatcgggat ccatggcctc cctgctgacc gagaccatgc ccttcaagac caccatcgag
      181 ggcaccgtga acggccactg cttcaagtgc atcggcaagg gcgagggcaa ccccttcgag
      241 ggcacccagg agatgaagat cgaggtgatc gagggcggcc ccctgccctt cgccttccac
      301 atcctgtcca cctcctgcat gtacggctcc aagaccttca tcaagtacgt gtccggcatc
      361 cccgactact tcaagcagtc cttccccgag ggcttcacct gggagcgcac caccacctac
      421 gaggacggcg gcttcctgac cgcccaccag gacacctccc tggacggcga ctgcctggtg
      481 tacaaggtga agatcctggg taacaacttc cccgccgacg gccccgtgat gcagaacaag
      541 gtcggccgct gggagcccgg aaccgagatc gtgtacgagg tggacggcgt gctgcgcggc
      601 cagtccctga tggccctgaa gtgccccggc ggccggcacc tgacctgcca cctgcacacc
      661 acctaccgct ccaagaagcc cgcctccgcc ctgaagatgc ccggcttcca cttcgaggac
      721 caccgcatcg agatcatgga ggaggtggag aagggcaagt gctacaagca gtacgaggcc
      781 gccgtgggcc gctactgcga cgccgccccc tccaagctgg gccacaactg aagcttaatt
      841 agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
      901 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gctagcttgg
      961 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     1021 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     1081 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     1141 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     1201 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     1261 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     1321 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     1381 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     1441 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     1501 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     1561 ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     1621 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
     1681 cgcctggggt aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa
     1741 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     1801 ccgccgctct agagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     1861 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     1921 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     1981 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     2041 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     2101 cttccgcttc ctcgctcact gactcgctgc gctcggtctg tcggctgcgg cgagcggtat
     2161 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2461 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2701 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2881 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2941 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3001 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3061 aggcacctat ctcagcgatc tgtctatttc gttcatccat agctgcctga ctccccgtcg
     3121 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3181 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3241 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3301 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3361 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3421 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3481 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3541 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3601 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3661 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3721 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3781 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3841 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3901 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3961 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4021 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4081 gtatcacgag gccctttcgt cttcac
//
