LOCUS       62056_13400        4801 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             654..671
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             753..1463
                     /codon_start=1
                     /label=KillerRed
                     /note="red fluorescent protein KillerRed, a
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /translation="SEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFNVH
                     AVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDGTM
                     TSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLAFI
                     GFTTADGGLMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQMRDTSDKRDHVCQREVAYA
                     HSVPRITSAIGSDED"
     polyA_signal    1589..1710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1717..2172)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2199..2303
                     /label=AmpR promoter
     promoter        2305..2662
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2697..3488
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3723..3770
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4099..4687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc atgcatcatc
      661 accatcacca tacggatcca ccggtcgcca ccatgctgtg ctgtatgaga agaaccaaac
      721 aggttgaaaa gaatgatgag gaccaaaaga tctccgaggg cggccccgcc ctgttccaga
      781 gcgacatgac cttcaaaatc ttcatcgacg gcgaggtgaa cggccagaag ttcaccatcg
      841 tggccgacgg cagcagcaag ttcccccacg gcgacttcaa cgtgcacgcc gtgtgcgaga
      901 ccggcaagct gcccatgagc tggaagccca tctgccacct gatccagtac ggcgagccct
      961 tcttcgcccg ctaccccgac ggcatcagcc atttcgccca ggagtgcttc cccgagggcc
     1021 tgagcatcga ccgcaccgtg cgcttcgaga acgacggcac catgaccagc caccacacct
     1081 acgagctgga cgacacctgc gtggtgagcc gcatcaccgt gaactgcgac ggcttccagc
     1141 ccgacggccc catcatgcgc gaccagctgg tggacatcct gcccaacgag acccacatgt
     1201 tcccccacgg ccccaacgcc gtgcgccagc tggccttcat cggcttcacc accgccgacg
     1261 gcggcctgat gatgggccac ttcgacagca agatgacctt caacggcagc cgcgccatcg
     1321 agatccccgg cccacacttc gtgaccatca tcaccaagca gatgagggac accagcgaca
     1381 agcgcgacca cgtgtgccag cgcgaggtgg cctacgccca cagcgtgccc cgcatcacca
     1441 gcgccatcgg tagcgacgag gattaaagcg gccgcgactc tagatcataa tcagccatac
     1501 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1561 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1621 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1681 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     1741 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1801 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1861 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1921 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     1981 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2041 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2101 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2161 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2221 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2281 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     2341 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2401 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2461 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2521 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2581 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2641 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     2701 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2761 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     2821 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     2881 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     2941 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3001 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3061 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3121 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3181 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     3241 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3301 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3361 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3421 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3481 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3541 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3601 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3661 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     3721 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     3781 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     3841 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     3901 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     3961 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4021 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4081 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4141 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4201 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     4261 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4321 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4381 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4441 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4501 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4561 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4621 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4681 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4741 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     4801 t
//
