LOCUS       pKatushka2S-B.        4138 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing Katushka2S in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pKatushka2S-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Luker KE, Pata P, Shemiakina II, Pereverzeva A, Stacer AC, Shcherbo 
            DS, Pletnev VZ, Skolnaja M, Lukyanov KA, Luker GD, Pata I, Chudakov 
            DM.
  TITLE     Comparative study reveals better far-red fluorescent protein for 
            whole body imaging.
  JOURNAL   Sci Rep 2015;5:10332.
  PUBMED    26035795
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4138)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2015"; volume: "5"; pages: "10332"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             96..107
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             114..116
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             147..851
                     /label=Katushka2S
                     /note="improved version of the oligomeric far-red
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
     terminator      884..978
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1022..1678
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1746..1832
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1988..2128
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2314..2902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3076..3933)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3934..4038)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttgataaaga ggagaaatta actatgagag
      121 gatcgggatc cttaccggtc gccaccatgg tgggtgagga tagcgtgctg atcaccgaga
      181 acatgcacat gaaactgtac atggagggca ccgtgaacga ccaccacttc aagtgcacat
      241 ccgagggcga aggcaagccc tacgagggca cccagaccat gaagatcaag gtggtcgagg
      301 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa
      361 cctttatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
      421 tcacatggga gaggatcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
      481 ccagcctcca gaacggctgc ctcatctaca acgtcaagat caacggggtg aacttcccat
      541 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccagcacc gagatgctgt
      601 accccgctga cagcggcctg agaggccatg cccagatggc cctgaagctc gtgggcgggg
      661 gctacctgca ctgctccctc aagaccacat acagatccaa gaaacccgct aagaacctca
      721 agatgcccgg cttctacttc gtggaccgga gactggaaag aatcaaggag gccgacaaag
      781 agacctacgt cgagcagcac gagatggctg tggccagata ctgcgacctg cctagcaaac
      841 tggggcacag ctgagcggcc gcaagcttaa ttagctgagc ttggactcct gttgatagat
      901 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
      961 ttttttattg gtgagaatcc aagctagctt ggcgagattt tcaggagcta aggaagctaa
     1021 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     1081 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1141 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1201 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
     1261 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1321 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1381 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1441 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1501 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1561 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca
     1621 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1681 atttttttaa ggcagttatt ggtgccctta aacgcctggg gtaatgactc tctagcttga
     1741 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1801 tgtcggtgaa cgctctcctg agtaggacaa atccgccgct ctagagctgc ctcgcgcgtt
     1861 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     1921 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     1981 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     2041 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     2101 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     2161 gcgctcggtc tgtcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2221 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2281 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2341 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2401 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2461 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     2521 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2581 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2641 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2701 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     2761 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2821 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2881 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2941 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3001 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3061 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3121 tcgttcatcc atagctgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3181 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3241 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3301 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3361 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3421 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3481 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3541 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3601 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3661 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3721 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3781 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3841 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3901 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3961 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4021 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     4081 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcac
//
