LOCUS       40924_26541        5276 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKanCOs1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Oster CJ, Phillips GJ.
  TITLE     Vectors for ligation-independent construction of lacZ gene fusions 
            and cloning of PCR products using a nicking endonuclease
  JOURNAL   Plasmid 66 (3), 180-185 (2011)
  PUBMED    21854804
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Oster C, Phillips GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2011) Veterinary Microbiology, Iowa State 
            University, 1802 University Boulevard, Ames, IA 50010, USA
REFERENCE   3  (bases 1 to 5276)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5276)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2011"; volume: "66"; issue: "3"; pages: "180-185"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2011) Veterinary Microbiology, Iowa State University, 1802 
            University Boulevard, Ames, IA 50010, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(127..143)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      847..1069
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1117..2064
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(3317..4129)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(4575..5030)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5172..5188
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5198..5216
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5242..5258
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aactctcgca ggcctgaatg tcgaggaaga gcgaagatct aaagccgaat tccagcacac
       61 tggcggccgt tactagtgga tccgagctcg gtaccaagct tgatatcgaa ttcctgcagc
      121 ccgggggatc cactagttct agagcggccg acgcgctggg ctacgtcttg ctggcgttcg
      181 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
      241 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
      301 aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg
      361 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
      421 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
      481 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccgcagaccc gccataaaac
      541 gccctgagaa gcccgtgacg ggcttttctt gtattatggg tagtttcctt gcatgaatcc
      601 ataaaaggcg cctgtagtgc catttacccc cattcactgc cagagccgtg agcgcagcga
      661 actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac aaaaggaata
      721 ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt ttaaggtctg
      781 ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg aactgactaa
      841 agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta ttctttcttt
      901 attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg tctagcggaa
      961 tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca gaatttacag
     1021 atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca tctcaattgg
     1081 tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt tgttttcaaa
     1141 gcaaatgaac tagcgattag tcgctatgac ttaacggagc atgaaaccaa gctaatttta
     1201 tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga acggacggta
     1261 tcgttcactt ataaccaata cgctcagatg atgaacatca gtagggaaaa tgcttatggt
     1321 gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag gaatcctttg
     1381 gttaaaggct ttaagatctt ccagtggaca aactatgcca agttctcaag cgaaaaatta
     1441 gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa attcataaaa
     1501 tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat ttatgagtgg
     1561 ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat tagccttgat
     1621 gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag gcttaaccaa
     1681 tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa attggtggtt
     1741 gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga tagacaaatg
     1801 gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa aataccaaca
     1861 accattacat cagattccta cctacataac ggactaagaa aaacactaca cgatgcttta
     1921 actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat gcaaagtaag
     1981 tatgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac actagagaac
     2041 atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta tcagtgaagc
     2101 atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa agggaaaact
     2161 gtccatatgc acagatgaaa acggtgtaaa aaagatagat acatcagagc ttttacgagt
     2221 ttttggtgca ttcaaagctg ttcaccatga acagatcgac aatgtaacag atgaacagca
     2281 tgtaacacct aatagaacag gtgaaaccag taaaacaaag caactagaac atgaaattga
     2341 acacctgaga caacttgtta cagctcaaca gtcacacata gacagcctga aacaggcgat
     2401 gctgcttatc gaatcaaagc tgccgacaac acgggagcca gtgacgcctc ccgtggggaa
     2461 aaaatcatgg caattctgga agaaatagcg ctttcagccg gcaaaccggc tgaagccgga
     2521 tctgcgattc tgataacaaa ctagcaacac cagaacagcc cgtttgcggg cagcaaaacc
     2581 cgtacttttg gacgttccgg cggttttttg tggcgagtgg tgttcgggcg gtgcgcgcaa
     2641 gatccattat gttaaacggg cgagtttaca tctcaaaacc gcccgcttaa caccatcaga
     2701 aatcctcagc gcgattttaa gcaccaaccc ccccccgtaa cacccaaatc catactgaaa
     2761 gtggctttgt tgaataaatc agatttcggg taagtctccc ccgtagcggg ttgtgttttc
     2821 aggcaatacg cacgctttca ggcatacctg ctttcgtcat tttgttcagc gctcgtacca
     2881 gggccatagc ctccgcaacc tgaccatcgt agtcacgcag cgtcagtgaa cccccgaaca
     2941 gagatctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tggtgtccga
     3001 cttatgcccg agaagatgtt tgagcaaact tatcgcttat ctgcttctca tagagtcttt
     3061 gcagacaaac tgccctgaat cgccccatca tccagccaga aagtgaggga gccacggttg
     3121 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa
     3181 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt
     3241 attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat
     3301 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     3361 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     3421 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     3481 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     3541 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     3601 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     3661 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     3721 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     3781 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     3841 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     3901 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     3961 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     4021 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     4081 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     4141 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     4201 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     4261 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc
     4321 aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc
     4381 tcactttctg gctggatgat ggggcgattc agggcagttt gtctgcaaga ctctatgaga
     4441 agcagataag cgataagttt gctcaacatc ttctcgggca taagtcggac accatgagcg
     4501 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4561 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     4621 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4681 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4741 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     4801 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4861 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4921 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4981 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     5041 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     5101 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     5161 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     5221 attgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgcag ctcttc
//
