LOCUS       40924_27164        3775 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKZ28, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Chromosomal expression of foreign and native genes from regulatable 
            promoters in Escherichia coli
  JOURNAL   Methods Mol. Biol. 267, 123-134 (2004)
  PUBMED    15269420
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2003) Biological Sciences, Purdue University, 
            Lilly Hall, West Lafayette, IN 47907, USA
REFERENCE   3  (bases 1 to 3775)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Mol. Biol. 267, 123-134 (2004)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2003) Biological Sciences, Purdue University, Lilly Hall, 
            West Lafayette, IN 47907, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      50..136
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      228..255
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      289..607
                     /note="araB; LT2"
                     /regulatory_class="promoter"
     CDS             616..1329
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      1416..1662
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    1676..1993
                     /label=phage Phi-80 attP
                     /note="attachment site of phage phi-80"
     rep_origin      complement(2104..2492)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2643..3299)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3300..3402)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    3599..3775
                     /label=oriT2
                     /note="oriT2"
ORIGIN
        1 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
       61 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      121 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
      181 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
      241 gacggatggc ctttttgcgt ggccagtgcc aagcttgcat gcctgcagcc gatgttcaga
      301 gaagaaacaa attgtccata tcgaccagga cgacagagct tccgtctccg caagactttg
      361 cgcttgatga aagcacgtat caaccccgct tgtgaaaagc gctttgtaac aaaagcgtac
      421 agttcaggcg ataaaattaa gtaacagaag tgtctataac tatggctgga atgtccacat
      481 tgaatatttg cacagcgtca cactttgcaa agcattagca tttttgtcca taagattagc
      541 ggatcctgcc tgacggtttt tgccgcgact ctctactgtt tctccatacc tgtttttctg
      601 gatggaggaa ttcatatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
      661 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      721 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      781 ccatggccaa cacttgtcac tactttcgcg tatggtcttc aatgctttgc gagataccca
      841 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      901 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      961 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     1021 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     1081 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     1141 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     1201 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     1261 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     1321 ctatacaaat aacgaagagc tgcaggtcga cggatccccg gaattaattc tcatgtttga
     1381 cagcttatca ctgatcagtg aattaatggc gatgacgcat cctcacgata atatccgggt
     1441 aggcgcaatc actttcgtct ctactccgtt acaaagcgag gctgggtatt tcccggcctt
     1501 tctgttatcc gaaatccact gaaagcacag cggctggctg aggagataaa taataaacga
     1561 ggggctgtat gcacaaagca tcttctgttg agttaagaac gagtatcgag atggcacata
     1621 gccttgctca aattggaatc aggtttgtgc caataccagt agaaacagac gaagaagcta
     1681 gctgataacg ggaaagaaac agagaagggc acaaatattg tgtactttaa tgtgcccttt
     1741 aatttattga ttggtggttg aattgtccgt aactttttga tttaagtgca aatttctaat
     1801 aaattagaac actttcttaa attgtcattt ggcatattac gaacaattcc gcgtaaaaac
     1861 gttctgttac gctaaaccct tatccagcag gctttcaagg atgtaaacca taacactctg
     1921 cgaactagtg ttacattgcg tgtagctttg agtgggcaac tttgtgtaca cttttgtgta
     1981 cccaaaaaca aaaatgtgta cccattcaat gatcaccgac acaaagctca ggaaggcgct
     2041 cggcaagaaa agagatgata tcgagattat ttctgattcg cacgggccca tggctaattc
     2101 ccatgtcagc cgttaagtgt tcctgtgtca ctgaaaattg ctttgagagg ctctaagggc
     2161 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa
     2221 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg
     2281 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct
     2341 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg
     2401 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac
     2461 tatcaacagg ttgaactgct gatcttcaga tcctctacgc cggacgcatc gtggccggat
     2521 cttgcggccg caaaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2581 cttggtctga cagttaccaa tgcttaatca gtgaggcacc aataactgcc ttaaaaaaat
     2641 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     2701 tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     2761 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     2821 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     2881 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     2941 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     3001 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     3061 ccgtctttca ttgccatacg gaatttcgga tgagcattca tcaggcgggc aagaatgtga
     3121 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     3181 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     3241 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     3301 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     3361 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa
     3421 aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag
     3481 tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta
     3541 ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagcat
     3601 cgatgattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt
     3661 gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag
     3721 gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgca
//
