LOCUS       40924_27159        3777 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKZ22, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Chromosomal expression of foreign and native genes from regulatable 
            promoters in Escherichia coli
  JOURNAL   Methods Mol. Biol. 267, 123-134 (2004)
  PUBMED    15269420
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2003) Biological Sciences, Purdue University, 
            Lilly Hall, West Lafayette, IN 47907, USA
REFERENCE   3  (bases 1 to 3777)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3777)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Mol. Biol. 267, 123-134 (2004)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2003) Biological Sciences, Purdue University, Lilly Hall, 
            West Lafayette, IN 47907, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      50..136
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      228..255
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      289..607
                     /note="araB; LT2"
                     /regulatory_class="promoter"
     CDS             618..1331
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      1418..1664
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    1678..1995
                     /label=phage Phi-80 attP
                     /note="attachment site of phage phi-80"
     rep_origin      complement(2106..2494)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2645..3301)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3302..3404)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    3601..3777
                     /label=oriT2
                     /note="oriT2"
ORIGIN
        1 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
       61 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      121 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
      181 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
      241 gacggatggc ctttttgcgt ggccagtgcc aagcttgcat gcctgcagcc gatgttcaga
      301 gaagaaacaa attgtccata tcgaccagga cgacagagct tccgtctccg caagactttg
      361 cgcttgatga aagcacgtat caaccccgct tgtgaaaagc gctttgtaac aaaagcgtac
      421 agttcaggcg ataaaattaa gtaacagaag tgtctataac tatggctgga atgtccacat
      481 tgaatatttg cacagcgtca cactttgcaa agcattagca tttttgtcca taagattagc
      541 ggatcctgcc tgacggtttt tgccgcgact ctctactgtt tctccatacc tgtttttctg
      601 gatggaggag ctcttcaatg agtaaaggag aagaactttt cactggagtt gtcccaattc
      661 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      721 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      781 ttccatggcc aacacttgtc actactttcg cgtatggtct tcaatgcttt gcgagatacc
      841 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      901 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      961 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     1021 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     1081 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     1141 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1201 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1261 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1321 aactatacaa ataacgaaga gctgcaggtc gacggatccc cggaattaat tctcatgttt
     1381 gacagcttat cactgatcag tgaattaatg gcgatgacgc atcctcacga taatatccgg
     1441 gtaggcgcaa tcactttcgt ctctactccg ttacaaagcg aggctgggta tttcccggcc
     1501 tttctgttat ccgaaatcca ctgaaagcac agcggctggc tgaggagata aataataaac
     1561 gaggggctgt atgcacaaag catcttctgt tgagttaaga acgagtatcg agatggcaca
     1621 tagccttgct caaattggaa tcaggtttgt gccaatacca gtagaaacag acgaagaagc
     1681 tagctgataa cgggaaagaa acagagaagg gcacaaatat tgtgtacttt aatgtgccct
     1741 ttaatttatt gattggtggt tgaattgtcc gtaacttttt gatttaagtg caaatttcta
     1801 ataaattaga acactttctt aaattgtcat ttggcatatt acgaacaatt ccgcgtaaaa
     1861 acgttctgtt acgctaaacc cttatccagc aggctttcaa ggatgtaaac cataacactc
     1921 tgcgaactag tgttacattg cgtgtagctt tgagtgggca actttgtgta cacttttgtg
     1981 tacccaaaaa caaaaatgtg tacccattca atgatcaccg acacaaagct caggaaggcg
     2041 ctcggcaaga aaagagatga tatcgagatt atttctgatt cgcacgggcc catggctaat
     2101 tcccatgtca gccgttaagt gttcctgtgt cactgaaaat tgctttgaga ggctctaagg
     2161 gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt
     2221 aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt
     2281 tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag
     2341 cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca
     2401 tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt
     2461 actatcaaca ggttgaactg ctgatcttca gatcctctac gccggacgca tcgtggccgg
     2521 atcttgcggc cgcaaaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2581 aacttggtct gacagttacc aatgcttaat cagtgaggca ccaataactg ccttaaaaaa
     2641 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2701 catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2761 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2821 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2881 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2941 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     3001 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     3061 caccgtcttt cattgccata cggaatttcg gatgagcatt catcaggcgg gcaagaatgt
     3121 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     3181 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     3241 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     3301 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     3361 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     3421 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     3481 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
     3541 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
     3601 atcgatgatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt
     3661 ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat
     3721 aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgca
//
