LOCUS       40924_27154        3984 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKZ20, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Chromosomal expression of foreign and native genes from regulatable 
            promoters in Escherichia coli
  JOURNAL   Methods Mol. Biol. 267, 123-134 (2004)
  PUBMED    15269420
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2003) Biological Sciences, Purdue University, 
            Lilly Hall, West Lafayette, IN 47907, USA
REFERENCE   3  (bases 1 to 3984)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3984)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Mol. Biol. 267, 123-134 (2004)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2003) Biological Sciences, Purdue University, Lilly Hall, 
            West Lafayette, IN 47907, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      50..136
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      228..255
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      289..607
                     /note="araB; LT2"
                     /regulatory_class="promoter"
     CDS             674..1531
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      1625..1871
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    1885..2202
                     /label=phage Phi-80 attP
                     /note="attachment site of phage phi-80"
     rep_origin      complement(2313..2701)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2852..3508)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3509..3611)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    3808..3984
                     /label=oriT2
                     /note="oriT2"
ORIGIN
        1 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
       61 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      121 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
      181 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
      241 gacggatggc ctttttgcgt ggccagtgcc aagcttgcat gcctgcagcc gatgttcaga
      301 gaagaaacaa attgtccata tcgaccagga cgacagagct tccgtctccg caagactttg
      361 cgcttgatga aagcacgtat caaccccgct tgtgaaaagc gctttgtaac aaaagcgtac
      421 agttcaggcg ataaaattaa gtaacagaag tgtctataac tatggctgga atgtccacat
      481 tgaatatttg cacagcgtca cactttgcaa agcattagca tttttgtcca taagattagc
      541 ggatcctgcc tgacggtttt tgccgcgact ctctactgtt tctccatacc tgtttttctg
      601 gatggaggag ctcttcaatg tcatgagaca ataaccctga taaatgcttc aataatattg
      661 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      721 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      781 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      841 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      901 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
      961 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1021 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1081 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1141 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1201 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1261 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1321 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1381 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1441 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1501 tgagataggt gcctcactga ttaagcattg gtaactgtta acgaagagct gcaggtcgac
     1561 ggatccccgg aattaattct catgtttgac agcttatcac tgatcagtga attaatggcg
     1621 atgacgcatc ctcacgataa tatccgggta ggcgcaatca ctttcgtctc tactccgtta
     1681 caaagcgagg ctgggtattt cccggccttt ctgttatccg aaatccactg aaagcacagc
     1741 ggctggctga ggagataaat aataaacgag gggctgtatg cacaaagcat cttctgttga
     1801 gttaagaacg agtatcgaga tggcacatag ccttgctcaa attggaatca ggtttgtgcc
     1861 aataccagta gaaacagacg aagaagctag ctgataacgg gaaagaaaca gagaagggca
     1921 caaatattgt gtactttaat gtgcccttta atttattgat tggtggttga attgtccgta
     1981 actttttgat ttaagtgcaa atttctaata aattagaaca ctttcttaaa ttgtcatttg
     2041 gcatattacg aacaattccg cgtaaaaacg ttctgttacg ctaaaccctt atccagcagg
     2101 ctttcaagga tgtaaaccat aacactctgc gaactagtgt tacattgcgt gtagctttga
     2161 gtgggcaact ttgtgtacac ttttgtgtac ccaaaaacaa aaatgtgtac ccattcaatg
     2221 atcaccgaca caaagctcag gaaggcgctc ggcaagaaaa gagatgatat cgagattatt
     2281 tctgattcgc acgggcccat ggctaattcc catgtcagcc gttaagtgtt cctgtgtcac
     2341 tgaaaattgc tttgagaggc tctaagggct tctcagtgcg ttacatccct ggcttgttgt
     2401 ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt ttcttataaa
     2461 acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt gttcaaacat
     2521 gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc ttagtacgtt
     2581 agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat gagagcttag
     2641 tacgtgaaac atgagagctt agtacgtact atcaacaggt tgaactgctg atcttcagat
     2701 cctctacgcc ggacgcatcg tggccggatc ttgcggccgc aaaaattaaa aatgaagttt
     2761 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2821 tgaggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     2881 gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca tgatgaacct
     2941 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     3001 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     3061 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     3121 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     3181 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     3241 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aatttcggat
     3301 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     3361 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     3421 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     3481 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     3541 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     3601 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     3661 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     3721 aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
     3781 gctccagtgg cttctgtttc tatcagcatc gatgattata gcgatttttt cggtatatcc
     3841 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
     3901 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
     3961 cttcttcact gtcccttatt cgca
//
