LOCUS       V005159                 8030 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005159
VERSION     V005159
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Wu Z.
  TITLE     Single large serine recombinase mediates the horizontal transfer of
            SCCmec in staphylococci
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8030)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8030)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-FEB-2017) College of Animal Science "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(956..1813)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1814..1918)
                     /label="AmpR promoter"
     primer_bind     2392..2408
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3456..4055
                     /codon_start=1
                     /product="RepC"
                     /label="RepC"
                     /note="repC; temperature sensitive replicon"
                     /protein_id="ASN64775.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     CDS             complement(4391..5014)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     protein_bind    5036..5054
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    5121..5139
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     misc_feature    complement(5144..5716)
                     /label="antisense-secY"
                     /note="antisense-secY"
     CDS             6410..7057
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    7240..7283
                     /label="MCS"
                     /note="MCS"
     CDS             7532..7930
                     /codon_start=1
                     /product="bleomycin resistance protein"
                     /label="bleomycin resistance protein"
                     /note="bleoR"
                     /protein_id="ASN64778.1"
                     /translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRI
                     HLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSL
                     KDQWWDERDFAVIDPDNNLISFFQQIKS"
ORIGIN
        1 ctcgacggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
       61 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      961 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1021 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1081 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1141 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1201 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1261 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1321 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1381 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1441 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1501 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1561 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1621 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1681 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1741 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1801 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1861 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1921 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     1981 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2041 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2101 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2161 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     2221 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     2281 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     2341 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     2401 cggccagtgc caagcttgca tgcctgcaga acggattgtt gatgattacg aaaatattaa
     2461 gagcacagac tattacacag aaaatcaaga attaaaaaaa cgtagagaga gtttgaaaga
     2521 agtagtgaat acatggaaag aggggtatca cgaaaaaagt aaagaggtta ataaattaaa
     2581 gcgagagaat gatagtttga atgagcagtt gaatgtatca gagaaatttc aagatagtac
     2641 agtgacttta tatcgtgctg cgagggcgaa tttccctggg tttgagaaag ggtttaatag
     2701 gcttaaagag aaattcttta atgattccaa attcgagcgt gtgggacagt ttatggatgt
     2761 tgtacaggat aatgtccaga aggtcgatag aaagcgtgag aaacagcgta cagacgattt
     2821 agagatgtag aggtactttt atgccgagaa aactttttgc gtgtgacagt ccttaaaata
     2881 tacttagagc gtaagcgaaa gtagtagcga cagctattaa ctttcggttg caaagctcta
     2941 ggatttttaa tggacgcagc gcatcacacg caaaaaggaa attggaataa atgcgaaatt
     3001 tgagatgtta attaaagacc tttttgaggt ctttttttct tagatttttg gggttattta
     3061 ggggagaaaa catagggggg tactacgacc tcccccctag gtgtccattg tccattgtcc
     3121 aaacaaataa ataaatattg ggtttttaat gttaaaaggt tgttttttat gttaaagtga
     3181 aaaaaacaga tgttgggagg tacagtgatg gttgtagata gaaaagaaga gaaaaaagtt
     3241 gctgttactt taagacttac aacagaagaa aatgagatat taaatagaat caaagaaaaa
     3301 tataatatta gcaaatcaga tgcaaccggt attctaataa aaaaatatgc aaaggaggaa
     3361 tacggtgcat tttaaacaaa aaaagataga cagcactggc atgctgccta tctatgacta
     3421 aattttgtta aatgtattag caccgttatt atatcatgag cgaaaatgta ataaaagaaa
     3481 ctgaaaacaa gaaaaattca agaggacgta attggacatt tgttttatat ccagaatcag
     3541 caaaagccga gtggttagag tatttaaaag agttacacat tcaatttgta gtgtctccat
     3601 tacatgatag ggatactgat acagaagata ggatgaaaaa agagcattat catattctag
     3661 tgatgtatga gggtaataaa tcttatgaac agataaaaat aattacagaa gaattgaatg
     3721 cgactattcc gcagattgca ggaagtgtga aaggtcttgt gagatatatg cttcacatgg
     3781 acgatcctaa taaatttaaa tatcaaaaag aagatatgat agtttatggc ggtgtagatg
     3841 ttgatgaatt attaaagaaa acaacaacag atagatataa attaattaaa gaaatgattg
     3901 agtttattga tgaacaagga atcgtagaat ttaagagttt aatggattat gcaatgaagt
     3961 ttaaatttga tgattggttc ccgcttttat gtgataactc ggcgtatgtt attcaagaat
     4021 atataaaatc aaatcggtat aaatctgacc gatagatttt gaatttaaga gtgtcacaag
     4081 acactctttt ttcgcaccag cgaaaactgg tttaagccga ctgcgcaaaa gacataatcg
     4141 attcacaaaa aataggcaca cgaaaaacaa gttaagggat gcagtttatg catcccttaa
     4201 cttacttatt aaataattta tagctattga aaagagataa gaattgttca aagctaatat
     4261 tgtttaaatc gtcaattcct gcatgtttta aggaattgtt aaattgattt tttgtaaata
     4321 ttttcttgta ttctttgtta tcttggttac cgtgaagtta ccatcacgga aaaaggttat
     4381 gctgctttta agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt
     4441 caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc
     4501 gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat
     4561 gctcttgatc ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata
     4621 atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt
     4681 catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta
     4741 gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt
     4801 ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca
     4861 aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg
     4921 cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct aatgcgctgt
     4981 taatcacttt acttttatct aatctagaca tcattaattc ctcctttttg ttgacactct
     5041 atcattgata gagttatttg tcaaactagt tttttatttg gatcccctcg agttcatgaa
     5101 aaactaaaaa aaatattgac actctatcat tgatagagta taattaaaat aagcttgatg
     5161 ggaatatgat aatagaaata ccattaccaa caccgaactg agtgatttga tcaccaagcc
     5221 atattaagaa agcagttcct gctgtcaaaa ctagtgctat taataaataa ctcataattg
     5281 actgattgat aatcagcgca cctttgagat aattattaaa ttggaatgcc atacctatag
     5341 attggataaa tgctaaagaa attgctaaat aacgagtaac gttatttaac tttcttctac
     5401 ctacttcacc ttgttttgcc cattctgaga atttagggac aatatccatt tgtaataatt
     5461 gcattacgat tgatgcagtg atgtagggta caatacccat tgcaaaaata gaaaatcgtt
     5521 tcaaggctcc gccaccaaaa gtatttaata actcagtggc accttgagaa ccttggggat
     5581 tatcaaaagc tgcaggattt actcctggag ctggtatata agtccctatt ttaaaaatta
     5641 ctaacattgc tagtgtgaag aaaatcttgt tacgaacctc ttttgttcta aagaagttca
     5701 caagggtttg aatcattaga tcaccccggg gtgatctaat gattcatatc tacatcaaat
     5761 gctggggcat atgttttacc ctcaggtaca ggtaagtcac ccgcttcaaa cgtcttctta
     5821 atatgcgtaa ttgcatctac tctaaatcca tcaatgcctt tatcaaacca ccagttcatc
     5881 atttcaaata cagcatctct aacttccgga ttaccccaat tcaaatcagg ttgtttttta
     5941 ctgaataaat ggaaataata ttgctcagta ttagcatcat attcccgcgc gccaattgag
     6001 ctcccctttc tatgtatgtt ttttactagt catttaaaac gatacattaa taggtacgaa
     6061 aaagcaactt tttttgcgct taaaaccagt cataccaata acttaagggt aactagcctc
     6121 gccggcaata gttaccctta ttatcaagat aagaaagaaa aggatttttc gctacgctca
     6181 aatcctttaa aaaaacacaa aagaccacat tttttaatgt ggtcttttat tcttcaacta
     6241 aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt aaaatatata
     6301 tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag aaagcagaca
     6361 agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa tgaaatttaa
     6421 taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca
     6481 aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca
     6541 agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa
     6601 tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga
     6661 gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt
     6721 aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg
     6781 ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat
     6841 tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct
     6901 acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc
     6961 tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat
     7021 tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa tgccgactgt
     7081 actttttaca gtcggttttc taatgtcact aacctgcccc gttagttgaa gaaggttttt
     7141 atattacagc tccagatcca tatccttctt tttctgaacc gacttctcct ttttcgcttc
     7201 tttattccaa ttgctttatt gacgttgagc ctcggaaccg gtaccggaga attccgagcg
     7261 gccgccggag atcttccgtc gacatgtgtc aaaacgcata ccattttgaa cgatgacctc
     7321 taataattgt taatcatgtt ggttacgtat ttattaactt ctcctagtat tagtaattat
     7381 catggctgtc atggcgcatt aacggaataa agggtgtgct taaatcgggc cattttgcgt
     7441 aataagaaaa aggattaatt atgagcgaat tgaattaata ataaggtaat agatttacat
     7501 tagaaaatga aaggggattt tatgcgtgag aatgttacag tctatcccgg cattgccagt
     7561 cggggatatt aaaaagagta taggttttta ttgcgataaa ctaggtttca ctttggttca
     7621 ccatgaagat ggattcgcag ttctaatgtg taatgaggtt cggattcatc tatgggaggc
     7681 aagtgatgaa ggctggcgct ctcgtagtaa tgattcaccg gtttgtacag gtgcggagtc
     7741 gtttattgct ggtactgcta gttgccgcat tgaagtagag ggaattgatg aattatatca
     7801 acatattaag cctttgggca ttttgcaccc caatacatca ttaaaagatc agtggtggga
     7861 tgaacgagac tttgcagtaa ttgatcccga caacaatttg attagctttt ttcaacaaat
     7921 aaaaagctaa aatctattat taatctgttc agcaatcggg cgcgattgct gaataaaaga
     7981 tacgagagac ctctcttgta tcttttttat tttgagtggt tttgtccgtt
//
