LOCUS       V005160                 9913 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005160
VERSION     V005160
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Wu Z.
  TITLE     Single large serine recombinase mediates the horizontal transfer of
            SCCmec in staphylococci
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9913)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9913)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-FEB-2017) College of Animal Science "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(956..1813)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1814..1918)
                     /label="AmpR promoter"
     primer_bind     2392..2408
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3456..4055
                     /codon_start=1
                     /gene="repC"
                     /product="temperature sensitive replication protein RepC"
                     /label="repC"
                     /protein_id="ASN64769.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     gene            3456..4055
                     /gene="repC"
                     /label="repC"
     CDS             complement(4391..5014)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     protein_bind    5036..5054
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    5121..5139
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     misc_feature    complement(5144..5716)
                     /label="antisense-secY"
                     /note="antisense-secY"
     CDS             6410..7057
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      7272..7370
                     /label="PmecA"
                     /note="PmecA"
                     /regulatory_class="promoter"
     CDS             7368..9128
                     /codon_start=1
                     /gene="ccrC2"
                     /product="cassette chromosome recombinase CcrC2"
                     /label="ccrC2"
                     /note="N-terminal attSCC tag"
                     /protein_id="ASN64772.1"
                     /translation="MGFFSKITDKQKHTINKTKKGAGATMKRKVAIYTRVSTKEQLKQG
                     YSIQQQADLLIKKAKQIFPDDEYEIYSDEGISGKNIEARPGMKRLLEDIENRQIKVVMS
                     WKLNRLSRSNRDIQNIIYEFRRGGAYYISISENIDTSTQNGEMMIGAFGLVAQIERETI
                     VSNVKMGMNAKAKQGEAITGRVLGYVLKPNPLTGKNELVIDEFEANIVRQIFDLYLNQN
                     KGLKAIANYLNKQGYHTINKKPFSVYGVKYILNNPVYKGYVRFNNYQNWAVERRSGKND
                     KSKVILVQGKHKAIIDEEIFDKTHEKLAAKSFKPGRPIGGDFWLRGLIKCPECGNNMVC
                     RRTYYNTKKSNERTIKRYYICSLFNRAGSAACHSNAIKAEVVERVVYFHLRRILSQPHV
                     VKTIATQVIDAMEKKKANQTPMSIDTNSLENQKKKLQKRKERLIDLYLDEEIDKETMQN
                     KQDKLNVQLEKIDEQLKQAEVMNKASQDIAIPNYKKIKGQLYVMLYRFTGYMKKANPEA
                     KNKLMHMLIDSIELTTDKQVKLIKYKIDESLLPQSIKKDCGNFFMPKFNFVINVTKKNR
                     IENLSLLPLF"
     gene            7368..9128
                     /gene="ccrC2"
                     /label="ccrC2"
     repeat_region   7382..7420
                     /gene="ccrC2"
                     /label="attSCC"
                     /note="attSCC"
     repeat_region   7399..7417
                     /gene="ccrC2"
                     /label="Direct repeat"
                     /note="Direct repeat"
     CDS             9415..9813
                     /codon_start=1
                     /gene="bleoR"
                     /product="bleomycin resistance protein"
                     /label="bleoR"
                     /protein_id="ASN64773.1"
                     /translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRI
                     HLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSL
                     KDQWWDERDFAVIDPDNNLISFFQQIKS"
     gene            9415..9813
                     /gene="bleoR"
                     /label="bleoR"
ORIGIN
        1 ctcgacggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
       61 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      961 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1021 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1081 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1141 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1201 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1261 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1321 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1381 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1441 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1501 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1561 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1621 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1681 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1741 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1801 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1861 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1921 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     1981 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2041 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2101 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2161 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     2221 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     2281 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     2341 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     2401 cggccagtgc caagcttgca tgcctgcaga acggattgtt gatgattacg aaaatattaa
     2461 gagcacagac tattacacag aaaatcaaga attaaaaaaa cgtagagaga gtttgaaaga
     2521 agtagtgaat acatggaaag aggggtatca cgaaaaaagt aaagaggtta ataaattaaa
     2581 gcgagagaat gatagtttga atgagcagtt gaatgtatca gagaaatttc aagatagtac
     2641 agtgacttta tatcgtgctg cgagggcgaa tttccctggg tttgagaaag ggtttaatag
     2701 gcttaaagag aaattcttta atgattccaa attcgagcgt gtgggacagt ttatggatgt
     2761 tgtacaggat aatgtccaga aggtcgatag aaagcgtgag aaacagcgta cagacgattt
     2821 agagatgtag aggtactttt atgccgagaa aactttttgc gtgtgacagt ccttaaaata
     2881 tacttagagc gtaagcgaaa gtagtagcga cagctattaa ctttcggttg caaagctcta
     2941 ggatttttaa tggacgcagc gcatcacacg caaaaaggaa attggaataa atgcgaaatt
     3001 tgagatgtta attaaagacc tttttgaggt ctttttttct tagatttttg gggttattta
     3061 ggggagaaaa catagggggg tactacgacc tcccccctag gtgtccattg tccattgtcc
     3121 aaacaaataa ataaatattg ggtttttaat gttaaaaggt tgttttttat gttaaagtga
     3181 aaaaaacaga tgttgggagg tacagtgatg gttgtagata gaaaagaaga gaaaaaagtt
     3241 gctgttactt taagacttac aacagaagaa aatgagatat taaatagaat caaagaaaaa
     3301 tataatatta gcaaatcaga tgcaaccggt attctaataa aaaaatatgc aaaggaggaa
     3361 tacggtgcat tttaaacaaa aaaagataga cagcactggc atgctgccta tctatgacta
     3421 aattttgtta aatgtattag caccgttatt atatcatgag cgaaaatgta ataaaagaaa
     3481 ctgaaaacaa gaaaaattca agaggacgta attggacatt tgttttatat ccagaatcag
     3541 caaaagccga gtggttagag tatttaaaag agttacacat tcaatttgta gtgtctccat
     3601 tacatgatag ggatactgat acagaagata ggatgaaaaa agagcattat catattctag
     3661 tgatgtatga gggtaataaa tcttatgaac agataaaaat aattacagaa gaattgaatg
     3721 cgactattcc gcagattgca ggaagtgtga aaggtcttgt gagatatatg cttcacatgg
     3781 acgatcctaa taaatttaaa tatcaaaaag aagatatgat agtttatggc ggtgtagatg
     3841 ttgatgaatt attaaagaaa acaacaacag atagatataa attaattaaa gaaatgattg
     3901 agtttattga tgaacaagga atcgtagaat ttaagagttt aatggattat gcaatgaagt
     3961 ttaaatttga tgattggttc ccgcttttat gtgataactc ggcgtatgtt attcaagaat
     4021 atataaaatc aaatcggtat aaatctgacc gatagatttt gaatttaaga gtgtcacaag
     4081 acactctttt ttcgcaccag cgaaaactgg tttaagccga ctgcgcaaaa gacataatcg
     4141 attcacaaaa aataggcaca cgaaaaacaa gttaagggat gcagtttatg catcccttaa
     4201 cttacttatt aaataattta tagctattga aaagagataa gaattgttca aagctaatat
     4261 tgtttaaatc gtcaattcct gcatgtttta aggaattgtt aaattgattt tttgtaaata
     4321 ttttcttgta ttctttgtta tcttggttac cgtgaagtta ccatcacgga aaaaggttat
     4381 gctgctttta agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt
     4441 caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc
     4501 gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat
     4561 gctcttgatc ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata
     4621 atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt
     4681 catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta
     4741 gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt
     4801 ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca
     4861 aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg
     4921 cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct aatgcgctgt
     4981 taatcacttt acttttatct aatctagaca tcattaattc ctcctttttg ttgacactct
     5041 atcattgata gagttatttg tcaaactagt tttttatttg gatcccctcg agttcatgaa
     5101 aaactaaaaa aaatattgac actctatcat tgatagagta taattaaaat aagcttgatg
     5161 ggaatatgat aatagaaata ccattaccaa caccgaactg agtgatttga tcaccaagcc
     5221 atattaagaa agcagttcct gctgtcaaaa ctagtgctat taataaataa ctcataattg
     5281 actgattgat aatcagcgca cctttgagat aattattaaa ttggaatgcc atacctatag
     5341 attggataaa tgctaaagaa attgctaaat aacgagtaac gttatttaac tttcttctac
     5401 ctacttcacc ttgttttgcc cattctgaga atttagggac aatatccatt tgtaataatt
     5461 gcattacgat tgatgcagtg atgtagggta caatacccat tgcaaaaata gaaaatcgtt
     5521 tcaaggctcc gccaccaaaa gtatttaata actcagtggc accttgagaa ccttggggat
     5581 tatcaaaagc tgcaggattt actcctggag ctggtatata agtccctatt ttaaaaatta
     5641 ctaacattgc tagtgtgaag aaaatcttgt tacgaacctc ttttgttcta aagaagttca
     5701 caagggtttg aatcattaga tcaccccggg gtgatctaat gattcatatc tacatcaaat
     5761 gctggggcat atgttttacc ctcaggtaca ggtaagtcac ccgcttcaaa cgtcttctta
     5821 atatgcgtaa ttgcatctac tctaaatcca tcaatgcctt tatcaaacca ccagttcatc
     5881 atttcaaata cagcatctct aacttccgga ttaccccaat tcaaatcagg ttgtttttta
     5941 ctgaataaat ggaaataata ttgctcagta ttagcatcat attcccgcgc gccaattgag
     6001 ctcccctttc tatgtatgtt ttttactagt catttaaaac gatacattaa taggtacgaa
     6061 aaagcaactt tttttgcgct taaaaccagt cataccaata acttaagggt aactagcctc
     6121 gccggcaata gttaccctta ttatcaagat aagaaagaaa aggatttttc gctacgctca
     6181 aatcctttaa aaaaacacaa aagaccacat tttttaatgt ggtcttttat tcttcaacta
     6241 aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt aaaatatata
     6301 tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag aaagcagaca
     6361 agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa tgaaatttaa
     6421 taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca
     6481 aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca
     6541 agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa
     6601 tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga
     6661 gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt
     6721 aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg
     6781 ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat
     6841 tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct
     6901 acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc
     6961 tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat
     7021 tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa tgccgactgt
     7081 actttttaca gtcggttttc taatgtcact aacctgcccc gttagttgaa gaaggttttt
     7141 atattacagc tccagatcca tatccttctt tttctgaacc gacttctcct ttttcgcttc
     7201 tttattccaa ttgctttatt gacgttgagc ctcggaaccg gtacctatcc taacagcaca
     7261 agagcgtcga ccttctacac ctccatatca caaaaattat aacattattt tgacataaat
     7321 actacatttg taatatacta caaatgtagt cttatataag gaggataatg ggttttttta
     7381 gcaaaatcac tgataagcag aagcatacca taaataaaac taaaaaaggg gcaggagcaa
     7441 ccatgaagcg caaagtggct atttatacaa gagtaagtac caaagaacaa ttgaaacaag
     7501 gctattctat tcaacaacaa gcagacttac tgattaaaaa agcaaagcaa atatttccgg
     7561 atgatgaata tgaaatttat agtgatgaag gtatctctgg taagaatatc gaagcaagac
     7621 ctggaatgaa aagattatta gaagatatag aaaatcgaca aattaaggtt gtgatgagtt
     7681 ggaaacttaa tcgtctttca cgttctaatc gagatataca aaatattatt tacgaattta
     7741 gaagaggtgg cgcctattat atctctattt cagaaaacat tgatacatca acacaaaatg
     7801 gcgaaatgat gataggtgca tttggattag ttgcacagat agaacgagaa accattgtta
     7861 gtaacgtaaa gatgggaatg aatgcgaaag cgaaacaagg tgaagctatt acaggtaggg
     7921 tgctaggtta tgttttaaaa cctaatcctc taacaggtaa aaatgaactt gtcattgacg
     7981 aatttgaagc caatatagtc agacaaattt ttgacctgta tttaaatcaa aataaaggat
     8041 taaaagcaat tgcgaattat ttaaataaac agggctatca tacgataaat aaaaagccat
     8101 ttagcgtata tggagtgaaa tatattctga ataatcccgt gtacaaaggc tatgttcgat
     8161 ttaataatta tcaaaattgg gcagtggaac gacgaagtgg caaaaatgat aaaagtaaag
     8221 tgatattagt tcaaggtaag cacaaagcga ttattgatga agagattttc gataaaacac
     8281 atgagaagtt agctgctaaa agttttaaac ctggtcgacc aattggtggc gatttctggt
     8341 tgagagggtt aatcaaatgt cctgagtgtg gaaataatat ggtgtgtaga cgaacatatt
     8401 acaatacaaa gaaatcaaat gagagaacga ttaaacgtta ttacatttgt tcattattca
     8461 atcgtgcagg tagtgcagct tgtcatagta atgcgattaa ggcagaagta gtcgaacgtg
     8521 tggtatattt tcatttaaga cgcatcctat ctcaacctca tgtggtcaaa acgatcgcaa
     8581 cacaggttat cgatgctatg gaaaagaaaa aagcaaacca aacgccaatg agtattgata
     8641 caaattcatt agagaatcaa aagaaaaagt tacaaaaaag aaaagaacgg ttgattgatt
     8701 tatatttaga tgaagaaata gataaagaaa cgatgcaaaa caaacaagat aaacttaatg
     8761 tgcaactgga aaaaatagat gaacagttaa agcaggctga ggttatgaat aaagcatctc
     8821 aagatatagc catacctaat tataagaaaa taaaaggtca attatatgtg atgctatacc
     8881 gatttacagg ctacatgaaa aaagcaaatc ccgaagcaaa gaataaatta atgcatatgt
     8941 taatcgattc tatagaactt acgaccgaca aacaagttaa actgattaaa tataaaatag
     9001 atgaatctct tctcccacaa tccattaaaa aggattgtgg gaattttttt atgcctaaat
     9061 ttaattttgt gataaatgtc acaaagaaaa ataggattga aaatttatca cttttaccac
     9121 ttttttaata agaattccga gcggccgccg gagatcttcc gtcgacatgt gtcaaaacgc
     9181 ataccatttt gaacgatgac ctctaataat tgttaatcat gttggttacg tatttattaa
     9241 cttctcctag tattagtaat tatcatggct gtcatggcgc attaacggaa taaagggtgt
     9301 gcttaaatcg ggccattttg cgtaataaga aaaaggatta attatgagcg aattgaatta
     9361 ataataaggt aatagattta cattagaaaa tgaaagggga ttttatgcgt gagaatgtta
     9421 cagtctatcc cggcattgcc agtcggggat attaaaaaga gtataggttt ttattgcgat
     9481 aaactaggtt tcactttggt tcaccatgaa gatggattcg cagttctaat gtgtaatgag
     9541 gttcggattc atctatggga ggcaagtgat gaaggctggc gctctcgtag taatgattca
     9601 ccggtttgta caggtgcgga gtcgtttatt gctggtactg ctagttgccg cattgaagta
     9661 gagggaattg atgaattata tcaacatatt aagcctttgg gcattttgca ccccaataca
     9721 tcattaaaag atcagtggtg ggatgaacga gactttgcag taattgatcc cgacaacaat
     9781 ttgattagct tttttcaaca aataaaaagc taaaatctat tattaatctg ttcagcaatc
     9841 gggcgcgatt gctgaataaa agatacgaga gacctctctt gtatcttttt tattttgagt
     9901 ggttttgtcc gtt
//
