LOCUS       40924_27139        4037 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKZ14, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Chromosomal expression of foreign and native genes from regulatable 
            promoters in Escherichia coli
  JOURNAL   Methods Mol. Biol. 267, 123-134 (2004)
  PUBMED    15269420
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2003) Biological Sciences, Purdue University, 
            Lilly Hall, West Lafayette, IN 47907, USA
REFERENCE   3  (bases 1 to 4037)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4037)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Mol. Biol. 267, 123-134 (2004)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2003) Biological Sciences, Purdue University, Lilly Hall, 
            West Lafayette, IN 47907, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      50..136
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      228..255
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      289..607
                     /note="araB; LT2"
                     /regulatory_class="promoter"
     promoter        635..726
                     /label=AmpR promoter
     CDS             727..1584
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      1678..1924
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    1938..2255
                     /label=phage Phi-80 attP
                     /note="attachment site of phage phi-80"
     rep_origin      complement(2366..2754)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2905..3561)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3562..3664)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    3861..4037
                     /label=oriT2
                     /note="oriT2"
ORIGIN
        1 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
       61 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      121 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
      181 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
      241 gacggatggc ctttttgcgt ggccagtgcc aagcttgcat gcctgcagcc gatgttcaga
      301 gaagaaacaa attgtccata tcgaccagga cgacagagct tccgtctccg caagactttg
      361 cgcttgatga aagcacgtat caaccccgct tgtgaaaagc gctttgtaac aaaagcgtac
      421 agttcaggcg ataaaattaa gtaacagaag tgtctataac tatggctgga atgtccacat
      481 tgaatatttg cacagcgtca cactttgcaa agcattagca tttttgtcca taagattagc
      541 ggatcctgcc tgacggtttt tgccgcgact ctctactgtt tctccatacc tgtttttctg
      601 gatggaggaa ttcatatgtg aagagcaacc cctatttgtt tatttttcta aatacattca
      661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      961 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1021 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1081 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1141 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1201 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1261 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1321 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1381 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1441 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1501 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1561 ggtgcctcac tgattaagca ttggtaactg ttaacgaaga gctgcaggtc gacggatccc
     1621 cggaattaat tctcatgttt gacagcttat cactgatcag tgaattaatg gcgatgacgc
     1681 atcctcacga taatatccgg gtaggcgcaa tcactttcgt ctctactccg ttacaaagcg
     1741 aggctgggta tttcccggcc tttctgttat ccgaaatcca ctgaaagcac agcggctggc
     1801 tgaggagata aataataaac gaggggctgt atgcacaaag catcttctgt tgagttaaga
     1861 acgagtatcg agatggcaca tagccttgct caaattggaa tcaggtttgt gccaatacca
     1921 gtagaaacag acgaagaagc tagctgataa cgggaaagaa acagagaagg gcacaaatat
     1981 tgtgtacttt aatgtgccct ttaatttatt gattggtggt tgaattgtcc gtaacttttt
     2041 gatttaagtg caaatttcta ataaattaga acactttctt aaattgtcat ttggcatatt
     2101 acgaacaatt ccgcgtaaaa acgttctgtt acgctaaacc cttatccagc aggctttcaa
     2161 ggatgtaaac cataacactc tgcgaactag tgttacattg cgtgtagctt tgagtgggca
     2221 actttgtgta cacttttgtg tacccaaaaa caaaaatgtg tacccattca atgatcaccg
     2281 acacaaagct caggaaggcg ctcggcaaga aaagagatga tatcgagatt atttctgatt
     2341 cgcacgggcc catggctaat tcccatgtca gccgttaagt gttcctgtgt cactgaaaat
     2401 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
     2461 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
     2521 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
     2581 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
     2641 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
     2701 aacatgagag cttagtacgt actatcaaca ggttgaactg ctgatcttca gatcctctac
     2761 gccggacgca tcgtggccgg atcttgcggc cgcaaaaatt aaaaatgaag ttttaaatca
     2821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2881 ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     2941 ttcattaagc attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc
     3001 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     3061 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     3121 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     3181 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     3241 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     3301 actatcccat atcaccagct caccgtcttt cattgccata cggaatttcg gatgagcatt
     3361 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     3421 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     3481 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     3541 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     3601 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     3661 atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc
     3721 aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc
     3781 gtcgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
     3841 tggcttctgt ttctatcagc atcgatgatt atagcgattt tttcggtata tccatccttt
     3901 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     3961 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     4021 actgtccctt attcgca
//
