LOCUS       40924_27134       14271 bp DNA     circular SYN 18-DEC-2018
DEFINITION  His-3 integration vector pKYAM026, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Construction of strains for rapid homokaryon purification after 
            integration of constructs at the histidine-3 (his-3) locus of 
            Neurospora crassa
  JOURNAL   Curr. Genet. 43 (1), 17-23 (2003)
  PUBMED    12684841
REFERENCE   2  (bases 1 to 14271)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2002) Biology, Texas A
REFERENCE   3  (bases 1 to 14271)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14271)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Genet."; date: "2003"; volume: "43"; issue: "1"; pages: "17-23"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-DEC-2002) Biology, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..2583)
                     /label=benomyl resistant marker
                     /note="benomyl resistant marker"
     promoter        complement(2590..2608)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2638..2654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2662..2678)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2686..2716)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2731..2752)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3040..3628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3802..4659)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4660..4764)
                     /label=AmpR promoter
     rep_origin      4791..5246
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5387..5403
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5432..5450
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             complement(10385..10396)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     misc_feature    10661..10713
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
ORIGIN
        1 ggccagcagt agacacttgg aatctaaaca gacattatca tcatcatgca acatgcatgt
       61 actgtctgat gtattaagag tataggggtc aacaactaga gtgtctgaca ggtatatcta
      121 gcagtttgat ttgaccattc ttttattaaa acactgtaac ccgcaacgaa gaactcctcc
      181 gcttcaagtc aagaatgcgc atatgtgcgt aaaatcgaca agcccggcgc attccaccga
      241 gcagggtatt gcctttggac tttacgtgcg cagaggagcc tgaatgttga gtggaatgat
      301 ttactcctcg ccctcaaggg gggcctcctc ctcgtactcc tcctcctcct cgtcaacacc
      361 agcatcctgg tactgctggt actcggagac gagatcgttc atgttggact cagcctcagt
      421 gaactccatc tcgtccatac cctcaccagt gtaccaatgc aagaaagcct tgcgcttgaa
      481 catggcagtg aactgctcgc cgatacgctt gaagagctcc tggatggcgg tggagttacc
      541 gacgaaggtg gaggacatct tgagaccgcg gggagggata gagcagaggg cagtctggac
      601 gttgttgggg attcactcga cgaagtagga agagttcttg ttctgaacgt tgcgcatctg
      661 gtcctcaacc tccttcatgg agaccttgcc acggctgttt gattcgtcag tattagtggt
      721 caaaagcagc attccatgat cagcaactta caagatggca gagcaggtga ggtaacgacc
      781 gttgcggaag tcagaagcag ccatcatgtt cttggggtcg aacatctgct gggtcaactc
      841 gggcacggag acggcacgga aatggtgggc gccgcggctg gtaaggggag cgaacccgac
      901 catgaagaag tggagacggg ggaagggaac catgttgacg gcgagcttgc ggagatcgga
      961 gttcagctgg ccggggaaac ggagggagac ggtgacaccg gacatgacgg cggagacgag
     1021 gtggttaaga tcgccgtaag aggggttgga gagcttgagg gtcctcatgc aaatgtcgta
     1081 aagcgcctcg ttgtcaatgc agaaggtctc gtcggagttc tcaacgagct gatggacgga
     1141 gagggtggcg ttgtagggct cgacaacggt atcggagacc ttgggggagg gcacgacgga
     1201 gtaggtagcc atcatgcggt cggggaactc ctcacgaatc ttggagataa ggagggtacc
     1261 cataccggca ccggtaccac caccgaggga gtgggtgatc tggaagccct ggaggcagtc
     1321 gcagccctca gcctcgcgac ggacgacatc gagaacctgg tcaacaagct cagcaccctc
     1381 agtgtagtga cccttggccc agttgttgcc agcaccggac tggccgaaga cgaagttatc
     1441 ggggcggaag agctggccga agggaccggc gcgaacggcg tccatggtac cgggctcgag
     1501 atcgacgagg acggcacgag ggacatactt gttgccggaa gcctgtcaag cgcgaagcta
     1561 gttagcgagc gaattaattc tcgaggaggg ggagatcgtc gagaccggtt gttgctcacc
     1621 tcgttgaagt agacgttcat gcgctcgagc tggagctcag aggtgccatt gtacctgtag
     1681 tgggcggtga gctttcgagg ccatcaccag atatggttat tgggcacgag gttacgtaca
     1741 caccggaggc atcgaggccg tgctcgccgg agatagtctg cctgtttatt ctgtcagcgt
     1801 cgggctccct cgctcaaagg tgtggttgtt cgcgatgatg acgacgtacc agaaagcagc
     1861 accgatttgg ttaccctgat cgtggctgtt gtgttagtcc tgctgttgtt gttggatcgc
     1921 gacgggggtg gaagccatca tcgacttacg cattggccgg tttggagatg aacctatagc
     1981 acgtgaagaa atcgtgtcag cgtcgatgtc cataatactc gcgatagatg gcagtccgcg
     2041 atggcgagaa agcgagcagg atcataatga gtcgggcagc agtaggtaga ggcacagaag
     2101 gggacgaggc agtttggtgg ggtagagcaa tcaggggtct cttgggaggg aaagagaaaa
     2161 aaaagaggga ttgctctgct gcgcggcaca gtaggcatca gagggagact tacaatttca
     2221 cgcatcttga cggtttggtg atgacgaaca cgggtctatg tggaggttga aggcagaaag
     2281 atggaagagc aaaaagatgg aagtggtgat aaagccgacg cggatagacg aagacgacgg
     2341 aggtcggtga aagtggagag gtttgatgtc tggcctaggg gagactggga agtcttggga
     2401 gtggaaaggg agatttggtg atttttggtg caagaaatga gcgggccaca gccggccagc
     2461 accagcttct cgttcaccgc cgccgccgct cgccgggctg ctgctgggtt gccggggcgc
     2521 ttgccaaagt ggggacatca ggcactgtca aacttttgga agggtggaag gccccctgtc
     2581 gacgagctcc ctatagtgag tcgtattaga ggccgacttg gccaaattcg taatcatgtc
     2641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     4801 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4861 cagcgcccta gggcccggtc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4921 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4981 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5041 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5101 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5161 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5221 taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa
     5281 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     5341 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     5401 agtgccaagc tgacttggtc agcggccgca gatttaggtg acactataga atatgcatca
     5461 ctagtaagct tgccatctcc accatcctct cgagcgtctg gaagtctgac cgtcccgatg
     5521 gtcttctccc caccgttgtc gttgatgagc acgacactgc tctgggtctg gtctacagca
     5581 gtgccgagag tgtgaacgag gccctcagga cacagactgg tgtctatcag agccggaagc
     5641 gcggtctctg gtacaagggt gctacttccg gagacactca ggagctcgtc cgcatctcgc
     5701 ttgactgcga taacgatgct ctcaagtttg tcgtgaagca gaagggtcgt ttctgccacc
     5761 tcgatcagtc cggctgcttt ggtcagctca aaggccttcc caagctcgag cagactttga
     5821 tttcgaggaa acagtctgcc cccgagggct cctacactgc ccgtctcttc tccgatgaga
     5881 agctagtccg ggccaagatc atggaggagg ctgaggagct ctgcaccgct cagacccccc
     5941 aggaaatcgc ctttgaggct gccgatctct tctactttgc tcttaccagg gccgttgctg
     6001 ccggcgttac tcttgccgat atcgaaagga gccttgacgc caagagctgg aaggtcaagc
     6061 gcaggactgg agatgctaag ggtaagtggg ctgagaagga gggcatcaag cctgcggcgt
     6121 ccgctctcgc tgccacttcg gcccctgtca ccaaggaggc cgcccaggag accacccctg
     6181 agaagatcac catgagacgt ttcgacgcct ccaaggtctc taccgaggag ctcgatgctg
     6241 ctctcaagcg tcctgcgcaa aagtcgtccg atgccatcta caagatcatt gtccccatca
     6301 tcgaggacgt ccgcaagaac ggcgacaagg ctgttctgtc gtacactcac aagttcgaga
     6361 aggctacctc tcttactagc cccgtcctga aggcgccctt ccccaaggag cttatgcagc
     6421 tccctgagga gaccattgct gccatcgacg tgtccttcga gaacatccgc aagttccacg
     6481 ccgcccagaa ggaggagaag cccctccagg tcgagaccat gcccggtgtt gtctgcagcc
     6541 gtttctctcg tcccatcgag gccgtcggct gctacatccc cggcggtacc gccgttctcc
     6601 ccagcactgc ccttatgctg ggtgttcccg ccatggtcgc cggctgcaac aagattgtgt
     6661 tcgcctctcc tccccgcgcc gacggaacca tcactcccga gattgtctac gtcgctcaca
     6721 aggttggcgc cgagtccatc gtgcttgccg gcggtgccca ggccgtagct gccatggcct
     6781 acggcaccga gagcatcacc aaggtcgaca agattctcgg ccccggtaac cagttcgtca
     6841 ctgctgccaa gatgttcgtc agcaacgaca ccaacgctgc cgttggtatt gacatgcccg
     6901 ctggcccgtc cgaggtgctg gtcatcgctg acaaggacgc caaccccgcg ttcgttgcct
     6961 cggatctcct gtcccaggct gagcacggcg ttgacagtca ggtcatcctg atcgctattg
     7021 acctcgacga ggagcatctt caggctattg aggacgaggt tcaccgtcag gctacggagc
     7081 ttcctcgcgt ccagattgtc cgtggctcca tcgcccactc gatcaccgtg caggtcaaga
     7141 ccgtcgagga ggccatggag ctcagcaaca agtacgctcc tgagcacttg atcctccaga
     7201 tcaaggaggc cgagaaggct gtcgatcttg tcatgaacgc cggtagtgtc ttcattggcg
     7261 cctggactcc tgagtccgtt ggcgattact ctgctggtgt taaccactcg ctgcgtaagt
     7321 tacatatcat aaatagcccc gcttcacaga ttcttctgct aacgtcaaga cacatagcta
     7381 cctatggctt tgccaagcag tactctggcg tcaatctcgc ctcgttcgtc aagcacatta
     7441 ccagctccaa cttgactgcc gagggtctca aaaacgtcgg ccaggctgtc atgcagttgg
     7501 ctaaggttga ggagctcgag gctcacagaa gggcggtcag catccgtctt gagcacatga
     7561 gcaagagcaa ctaaacggaa attcttttcg aagtagcaaa aaaaaaaaaa aaaaacaaga
     7621 acaaaaggat gtagtgggtt gatgtatatc tgggtcattt tgggcacata gagtaatgat
     7681 aacgagtttt ggacattgta ctgttctgta caggctgaag atcagtacat gaatctgttg
     7741 gtaagtgtag agacccaaac gtccctttag tttttctccc tattccagaa ggggtgctcg
     7801 tccctgggtg tttattttca ttattacatc aacttttttt tttttttttt ttttttcaga
     7861 tcatgcgtac atgaacgggg aagcacagac gatcgaaacg tggatgtcac aatgtcgctg
     7921 cagtgatgct gcattgcatg aagcgcccat ctcaatatac ttgcagtctt gcacgttgca
     7981 tgtgaacctc ccaaacaacc gaataaaaga cggcgaaaaa tgaagataaa aaaaaaccat
     8041 aaaaaaaatc agagggagtg tgggaaatgg tgtcttttag cattcagacc ccatagccgt
     8101 gcacgcccgg gtacagacag gttcatcgat gttgacattg actgggacac caggtctatc
     8161 tattttatct cctgtcctct accatacatc gggacatcgg acatcttgct gtacccccca
     8221 cacccacaaa gcctcataaa agcgccacac ccgaggaggt tcggtcggcc ccacgaactc
     8281 tgtgcctccc tgcctgttta cagggaccga acgctggaga atcttactag tttcctgaca
     8341 tccggcctac ccgagcagga aaagggacag ctcataggcg aggagggatt tgaagatggg
     8401 aacattttgg gtgattcgag aggaggaact aggtactgca tcatgatagt tcggggcagc
     8461 atcttggctg ggacattgtt aatacctcga tatgatgaag taggagggag tttttgcgtg
     8521 tcttgccgaa gtccagagat ctgttttatt ttatttttta tggatgtagt gtatcaacac
     8581 ccaagattcg gagaatagta ctaggattcg catttacaag tggaagtctt gagaatcgtt
     8641 gtatatcctt gtcttcctcg gaatgttaac aatcctacag cgagcgagcg agcggtcgga
     8701 tgcgctgatc tgataggcgc aatatacggc cgctttctcc ggtcgtgtag tgtaagctct
     8761 gtgggcatag tacactaaaa aaacccttgc atttcatgat ctgcctgcta ttcattccga
     8821 gctatttcag tggtcacatt tcgaggaaga aagaaagcaa ctaagattca cagccatcca
     8881 tccatccata tggaagaata atccattccc atgttccctc ccccccacta tgtatgtgac
     8941 cacacgctgc tgtcagaatg ccaacggtct caggtaccct cgtccgactg tttggcatgg
     9001 agttacatac actactagtg tagccccggg ccaagctacc ccgtcaaatc tatacatatc
     9061 tataacgggt ttcaggggtt tcgttcgctg tcaatcaagt ttgaaacatc actggggccg
     9121 ttggacggtg tattagacca ttggctccct cagctgtttg gcggctgggc ggctgggtca
     9181 aacggcaata acgggactcg agagggacga ggagagtcgg ttggctggct gcaatacaag
     9241 cgttcccacc taaccaacga gtcccgtttt ccatttgtgt gcctaaccat catctaggga
     9301 tgtcagggtt tggccggatc agggtatgtt tggttgactg ttgtcatgtc tgattgggta
     9361 catattatgg taggtgtctc gagaacagta gagtactcgg gcctagcgtt tggatgatta
     9421 cgcgagatat gagttgtggg ccgccatgca gttgcttgtc cataagcaga agttgctttg
     9481 ggatatattt ctcgtctttc aaaggtcacg aggtcctggg acgaacggca tcgccatcca
     9541 aagggttgaa catgagaaac ctgaatggcc tttgcgttga aatacaaaaa gtcaagaaca
     9601 aaatcgcttg aggataggga cgtggaagca agcaaatatg gtaagagagg tatacatcaa
     9661 ccctggttca attgttagcg tggttcttcc tccacgtcct cgttcatgac ggttaacagt
     9721 accaggctaa caattaaacc agggttgatg tgtactgata tgtaggtgct cagcaaactg
     9781 ccaatttctt ggccccaagc agcagtttgc tgtcagtgct gctcgtgtca gccttggtag
     9841 tggaacctaa actgctaaca cagcgcaagt gcgcatgtaa agatattgtg ggaggatctg
     9901 tatggatgga tgagattact gcttggtgtt ggttgcgagg cactgcggct gttaggcttt
     9961 gctgtgcccc gttcgacgaa gaaatacgcg gaactataaa ttggatacct agacttactg
    10021 cctatgggag gtatctaccg acgtagccga cggattctag caacatcccg actttgcttg
    10081 tagtgtacta tgatagcagc acagtgttgc tccttgtgag aatgggctct tttttttttt
    10141 tcccccttcc ctagggcgtt gactggactt gctctattgt tcccaaggta ggtgcccgtc
    10201 atcgattttc ccaagtctcc cgccagattg tcgtcatagt gtcatgatga cctcggtcgc
    10261 tggggctgcg tggttacggg gagctgggac cgctaggcct cagtggttgt gccattcagc
    10321 gtgggtgtgt ggagtagcgg tagaggcgct tggaagttgt gctagcggaa accctggaat
    10381 atcttctacc ctcgattcct tctcgggctg cccatgtgct gaggtgatgc cggggatctg
    10441 gcgccaatca tccattgagg ttcccgcagc ttcccggtgc cgcgcgcggg cgcagttgct
    10501 cacaggacac acctagacgc aggggcacag gggcaccgtt tggtgtgcaa ctgggtacct
    10561 agctgtagca agcactccac cgtctgtgca atcccccaat ccacggcagg aacttcgcac
    10621 cgccgcggca ccgagtgagc gaatccatcc gcattggatc gtttaaactc tagagcatgc
    10681 cccgggatcc tgatcatcga tgggcccgaa ttcaaaatac cacgggcgca ggtggtattg
    10741 gaggcttgtt gcagtccagc acatatttgt atgtaaagtg gttcttctta tctcgttttc
    10801 gagtgtcaac tgcgccagtt cagagttggg cggctgtgga cgaccttgct ggtgaacatg
    10861 tcttggactc catgcccctt cttcgtttcc tcaaatcaag aagtcgagga ccgtgaccgt
    10921 aaatcgctaa cgcaactcta gggccggact ttctggtggt ggctggcttg tcggcagttt
    10981 gttctccaac aacttcagca gcattgagac cctgctgagc gagaacaaag tctgggactt
    11041 tgagaactcc atctttaaag ggcccaagga ggctggcctt agtactgtca accgcattca
    11101 gtactggtcc gaagtggcaa aggaagttgc caagaagaag gatgctggct tcgagacaag
    11161 tataacagac tactggggcc gagcattgag ttaccaactg atcggagccg atatgggcgg
    11221 cccggcttac accttctcca gcattgccca gaccgacaac ttccagaagg ccgaaacgcc
    11281 gttccctatt ctggtagctg acggccgcgc gcctggagac accatcatct ccctcaatgc
    11341 taccaactac gagttcaacc cgttcgagac gggtagctgg gacccgaccg tctatggctt
    11401 tgcgccgacc aagtacctcg gcgccaactt cagcaacggc gtgatcccat cgggaggcaa
    11461 gtgcgttgag ggtctcgacc aagccggctt cgtcatgggc accagcagca cgctcttcaa
    11521 ccagttcctt ttggccaaca tctccagcta cgacggtgtg cccgacgtgc tcatcgaggc
    11581 cgtgacttct gtcctcaagg aaatcggcgc caagagggac gacgtctccc aaatcatccc
    11641 taatccgttc ctggactgga acaaccggac caaccccaac gccgacacgc tcgagctcga
    11701 cctggtcgac ggcggcgaag atctgcagaa tattccgctc aacccgctca cccaacccgt
    11761 gcgcgccgtg gacgtcatct tcgctgtcga ctcgtccgcc gacgtgacaa actggcccaa
    11821 tggcaccgcc ctgcgagcca cctacgagcg cactttcggc tctatttcca acgggacact
    11881 cttcccctcg atccccgacg actggacgtt tataaaccta ggcctcaaca accgcccctc
    11941 tttcttcggc tgcgatgtta agaactttac cttgaacgcc aaccaaaagg ttcccccctt
    12001 aatcgtctat gtccccaacg cgccctatac cgcgctgagc aacgtgtcca ccttcgatcc
    12061 gtcatacacc atgtctcagc gcaacgacat catcggcaac ggatggaact cagccacgca
    12121 gggaaacggc acgctggatt cggagtggcc cacttgcgtc gcctgcgcgg ttatcagcag
    12181 gagcttagat cggttgggca ggcagacgcc agccgcgtgc aagacttgct ttgagaggta
    12241 ttgctggaat ggcacagtga actcaaaaga tacaggggtt tacatgcctg agttcaagat
    12301 tgcggatgcg catgccctgg actcgggtgc tgttgctatc ggaaagatgg tgaatgtctg
    12361 gtcgtcggtt gtggtgggag ttgtggcggc tactttgttg ttgtaggggt aggggagacg
    12421 tgatgatatt ccagtctgat gaagttgaga ctggactgga gatcgccaag gatgcggagg
    12481 gaaaggaatg cgtggtgtta atgtcatgat ggatgaaggg tcatggatca tggaacgacg
    12541 gggcggggat attggatgat ggatatacca cactgcatgc atgctctatt gatagtatgc
    12601 tttggcattt acgtttaaca atcaattgct ccatcctgat gttctatctt tttcgacact
    12661 ggattgatac tactcctgtt gcttcgctct tgaggttgga aggaccttga ggttggaagg
    12721 accttgaggt tgtttgttct gagggaggtt atcgaagtat catctgtgct gatgccgatc
    12781 gatagactgc cctcttcttc gaggcaacga acggtcggat gagcctctaa tcatgatgct
    12841 cagtgccaca aaaaggctcc agcacagctg cccacacctt ttttgcctcg tcgctccttc
    12901 ctttttttcc ccccctttct tcctttccat ctcatcccgt accagagtgc ccaccgggta
    12961 tatatattac ctccttggcc gttctccttt gaccaataaa tcgcttggtc gagtggcgta
    13021 accgtttacc gtctacactt atcactcaaa ccaaaccaaa ccatcgaaga agttacctat
    13081 cggttcgagg gaacggtgat gttcttacgt tcaagtaacc caaagagcgt tccacatcgt
    13141 tgaccgtctc ctccagttct tggatctgtt taacttccgc agcgactgaa gaaggtaatc
    13201 actttttttt tttttggttc aaaaaaaaaa aaaaaaaatt acacgaaaag gttagaggcg
    13261 tttcgatggt taaactcgat cgagcggttg tttgtagcct tttcttttgt gtcttttggc
    13321 agtacttcct ttggaggtgg aaggagggtt agaggccgcg tcaacctaag taagggaggg
    13381 agctggttgc attcgaaccc cggatgggac ttgtgttctg ttgttgtttt tttttgcact
    13441 gatgatgatt ctgggcgagt gcgcttttga taactacagt cccacgtcat caacttcacg
    13501 attagtcctt gactcttttt tttctttttc tactaaaagc ctggtcttac ccaagtgttc
    13561 acggtatatg gagtatgcga tagagggcga tatatcttct caacaactcg tttcttctcg
    13621 ctcagcccac aatagggtgc ttgtaattct tccaccgaga gcaagtggga gtaatcttct
    13681 tcgatggcta cagccgtcag tcagaccacc tacatccacg tccaggcaat atctctggga
    13741 cttcacaaca acaaaagcgc actggaatga aacaggaaat ttctcgagat aagtgcctga
    13801 ttgtagtctt gcggttcatg tacatctgac cttttcttga gagcccccgt ttctggtcca
    13861 aaatcgtctg actttcacct agcctgcccc cacgaaatcc agtctcccct tgatgtcgtc
    13921 gggcaacgtc gagagatact cctcccaaat gtcctgattt gcacccgcgg gcacgcggaa
    13981 cgtgtaaata agtcctcctg ttcctccttt tcttccacta gcttcgtcat ctccagccat
    14041 caccgccaac tctcggttta tcttcggtgc cagaatgatg cttgatgtcc taggactgcc
    14101 cgcaaattgc agatacaagg gaacaggagg acagctacta agcggcggcg acaatgacat
    14161 tccccccacg cccgagccga gcagttcctg gactctagcc cccatggtca ggggagggga
    14221 tgttgtcact ctagcatgaa gatcagcgat cgagatgccc cccaaaagct t
//
