LOCUS       62056_13595        7447 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pKV, complete sequence.
ACCESSION   MK424960
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 7447)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..7447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..2546
                     /label=vermilion
                     /note="vermilion"
     protein_bind    2553..2586
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2587..3017
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     misc_feature    complement(3027..4407)
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     misc_feature    complement(4457..4501)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    4502..4932
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    4971..5040
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(5259..5277)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5298..5314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5322..5338)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5346..5376)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5391..5412)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5700..6288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6462..7319)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7320..7424)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccccattt gtgtgcgccg cactttcacc tcaagtgatt gataattccc agcctatctg
      721 gcagtgccca tcgcccagat caccgactgt gcaatcagtc ggaactggag ctctctcgct
      781 ctgttatcgg ttcgctgggg tctcatctcc ggtccgctgg cggagatcag ttcgccagca
      841 tccgccgctc gaggagtcac gatctgatct gagctgtgca ccatgagctg tccctatgca
      901 ggaaacgggt gagcaccagc acgtgctgtc caggaatgcc aatcgatctt cagttctgcg
      961 attcaattca aacccataca gaaacgatca cgatgattcg gcggtgccat taaccacgga
     1021 agtgggcaaa atctatggag agtatctgat gctggacaaa ctgctggatg cccagtgtat
     1081 gctgtccgag gaggacaagc gacccgtgca cgatgagcat ctgttcatca tcacgcacca
     1141 gggtgagtag gtttacaact ttgatgacaa cactcaatgg catttaagta ccttcgccac
     1201 agcctacgag ctttggttca agcagatcat ctttgagttc gactccatac gagacatgtt
     1261 ggatgcagag gtcatcgatg aaaccaagac gctggagatt gtcaagcgac tgaaccgagt
     1321 ggttctgatt ctaaaagtga gtgctttctg aatctcttac caaaatccgt ttataacttc
     1381 ctttgtacag ctcctggtgg accaagtgcc cattctggag accatgaccc cgctagactt
     1441 catggacttc cgcaagtacc tggcacccgc atctggtttt cagtcgctgc agttccgttt
     1501 gatcgagaac aagctgggag ttctgacaga gcagcgggtg agatacaacc agaagtactc
     1561 ggatgtcttt agcgacgagg aggcgcggaa ctcgattcgc aactcggaga aagatccctc
     1621 gctactggag ctagtgcagc gatggctgga gaggacgccc ggactggagg agagtggctt
     1681 caacttctgg gccaagtttc aggagagcgt cgatcgattc ctggaggcgc aggtacagag
     1741 cgccatggag gagcccgtgg agaaggcgaa aaactaccgc ctcatggaca ttgagaagcg
     1801 acgcgaggtg tatcgctcca tctttgatcc ggcagtgcac gatgcactgg tgcgtcgtgg
     1861 ggatcgccgg tttagccatc gtgcccttca gggagccatc atgatcacct tctataggga
     1921 tgaacccagg ttcagccaac cacaccagtt gctcaccctg ctcatggaca tcgactcgtt
     1981 aataaccaag tggagatgta agtattgcat tctttgatac tcttttataa atatatctta
     2041 tgtttaagac tggttttcct aaccaaatac tttctattcc cgccgcagac aatcacgtga
     2101 tcatggtgca acgcatgatt ggatcccaac agttgggcac tggtggctcg tctggatatc
     2161 aatatctgcg ctccactctc aggtgatcat cgcagatgtg attatatcgg ggatcaatga
     2221 actcaaactg ttctcccttt gttttttttt ggtttcagtg atcggtacaa ggtgtttctg
     2281 gatctgttca atctgtccac ttttctgatt ccccgcgagg cgattccacc gctggacgag
     2341 accattcgca agaaactgat caacaaaagt gtctgacaat cggcagggta tccaattcgt
     2401 caatgtttgg ctatgcgttg tttgttctgc ctactgtttt gtcgttttgg tgtaataaaa
     2461 ttacttgttt agtctttgtt atcacatttg atgtgttcct tttctttatg tctgacatat
     2521 aatacatata acataacaaa ataaatgcta gtataacttc gtataatgta tgctatacga
     2581 agttattggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     2641 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     2701 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     2761 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     2821 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     2881 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     2941 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3001 gtgtgccaac caacaacacg cgtacttcag ccaatccgcc gcaccctcag ctccaaagca
     3061 cgcatatgac ccgtgagcag gcagagaaac gcaggcggat aggagtcact agcgcagttc
     3121 tgtatagcct gcactatcaa gccgaagagc tggtagatat tgatgagcac atagtttctg
     3181 gtggagtgca tccagtcaac gccgaaccag gccggataca ggagctctcg atccgggcga
     3241 acgaccacgc ggacgcaact caaagtagtg ccaaatacga aaatactggc aaaggtgcgg
     3301 aaagtgccct gtgcgatatt cacttccttc ctcaaggcag aaatctcctc gggatcgccc
     3361 accagccggg ctctagcgtc catgagcctt aggagagagc gaatctcatg gagctgcttc
     3421 ctcaccatgt acacattcgc aaacttcaaa ttggccacaa tatcggtaat agttatggtc
     3481 aggttctcgc acaatccggc catgttggtg gtgaacagca gcctggccag caagctcaag
     3541 ggaaacagca cggtgaccac caagttgacc gtaatggagt ataccacgta aagcaggctt
     3601 gaaacgcccg gaggacgcat caggccagtg agctcccaaa cgcgccagtg gtagtacaca
     3661 gcactgtggg tgttcagttt gaaatcctct tgcttctcca ttgtctcggt tacctttggt
     3721 caaatccatg ggttctattg cctatatact cttgcgatta ccagtgattg cgctattagc
     3781 tattagatgg attgttggcc aaacttgtcg cttaagtggc tgggaattgt aaccgtaggc
     3841 ccgagtgtaa tgatccccca taaaaagttt tcgcaatgcc tttatttttt gttgcaaatc
     3901 tctctttatt ctgcggtatt cttcattatt gcggggatgg ggaaagtgtt tatatagaag
     3961 caacttacga ttgaacccaa atgcacctga caagcaaggt caaagggcca gatttttaaa
     4021 tatattattt agtcttagga ctctctattt gcaattaaat tactttgcta cctgagggtt
     4081 aaatcttccc cattgataat aataattcca ctatatgttc aattgggttt caccgcgctt
     4141 agttacatga cgagccctaa tgagccgtcg gtggtctata aactgtgcct tacaaatact
     4201 tgcaactctt ctcgttttga agtcagcaga gttattgcta attgctaatt gctaattgct
     4261 tttaactgat ttcttcgaaa ttggtgctat gtttatggcg ctattaacaa gtatgaatgt
     4321 caggtttaac caggggatgc ttaattgtgt tctcaacttc aaaggcagaa atgtttactc
     4381 ttgaccatgg gtttaggtat aatgttatca agctcctcga gttaacgtta cgttaacgtt
     4441 aacgttcgag gtcgactcta gactcgaggg taccagatct gaattcggat cctcaaggcc
     4501 ttggccacgt aataagtgtg cgttgaattt attcgcaaaa acattgcata ttttcggcaa
     4561 agtaaaattt tgttgcatac cttatcaaaa aataagtgct gcatactttt tagagaaacc
     4621 aaataatttt ttattgcata cccgttttta ataaaataca ttgcataccc tcttttaata
     4681 aaaaatattg catactttga cgaaacaaat tttcgttgca tacccaataa aagattatta
     4741 tattgcatac ccgtttttaa taaaatacat tgcataccct cttttaataa agaatattgc
     4801 atacgttgac gaaacaaatt ttcgttgcat acccaataaa agattattat attgcatacc
     4861 ttttcttgcc ataccattta gccgatcaat tgtgctcggc aacagtatat ttgtggtgtg
     4921 ccaaccaaca acactagaga tccactagtg tcgacgatgt aggtcacggt ctcgaagccg
     4981 cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc
     5041 tggtccatca tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc
     5101 accgggaagc cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt
     5161 gcgacggcgt cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg
     5221 gcgggcatgt cgacactagt tctagccagc ttttgttccc tttagtgagg gttaatttcg
     5281 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5341 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5401 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5461 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5521 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5581 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5641 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5701 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5761 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5821 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5881 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5941 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6001 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6061 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6121 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6181 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6421 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6481 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6541 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6601 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6661 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6721 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6781 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6841 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6901 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6961 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7021 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7081 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7141 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7201 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7261 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7321 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7381 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7441 gtgccac
//
