LOCUS       40924_27094        6180 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal transformation vector pKT313, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     New plasmids allowing positive-negative selection for fungal 
            transformation and efficient Cre-loxP mediated marker recycling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2018) Department of Applied Genetics and Cell 
            Biology, University of Natural Resources and Life Sciences Vienna 
            (BOKU), Konrad Lorenz Strasse 24, Tulln 3430, Austria
REFERENCE   3  (bases 1 to 6180)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6180)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2018) Department of Applied Genetics and Cell Biology, 
            University of Natural Resources and Life Sciences Vienna (BOKU), 
            Konrad Lorenz Strasse 24, Tulln 3430, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      62..808
                     /note="pki; Trichoderma reesei pyruvate kinase I promoter; 
                     accession CP016233"
                     /regulatory_class="promoter"
     regulatory      215..224
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             814..1941
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     CDS             1978..3000
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      3022..3751
                     /note="cbh2; Trichoderma reesei cellobiohydrolase II
                     terminator; accession CP016238"
                     /regulatory_class="terminator"
     promoter        complement(3818..3836)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4094..4682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4856..5713)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5714..5818)
                     /label=AmpR promoter
     promoter        6164..6180
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gcaggatcct taattaatcg aattctaggc cgcataggcc atactagtac
       61 tcgagataac ggtgagacta gcggccggtc cccttatccc agctgttcca cgttggcctg
      121 cccctcagtt agcgctcaac tcaatgcccc tcactggcga ggcgagggca aggatggagg
      181 ggcagcatcg cctgagttgg agcaaagcgg ccgggccgcc atgggagcag cgaaccaacg
      241 gagggatgcc gtgctttgtc gtggctgctg tggccaatcc gggcccttgg ttggctcaca
      301 gagcgttgct gtgagaccat gagctattat tgctaggtac agtatagaga gaggagagag
      361 agagagagag agagagaggg gaaaaaaggt gaggttgaag tgagaaaaaa aaaaaaaaaa
      421 aaaaatccaa ccactgacgg ctgccggctc tgccaccccc ctccctccac cccagaccac
      481 ctgcacactc agcgcgcagc atcacctaat cttggctcgc cttcccgcag ctcaggttgt
      541 tttttttttc tctctccctc gtcgaagccg cccttgttcc cttatttatt tccctctcca
      601 tccttgtctg cctttggtcc atctgcccct ttgtctgcat ctcttttgca cgcatcgcct
      661 tatcgtcgtc tcttttttca ctcacgggag cttgacgaag acctgactcg tgagcctcac
      721 ctgctgattt ctctcccccc ctcccgaccg gcttgacttt tgtttctcct ccagtacctt
      781 atcgcgaagc cggaagaacc tcttaacctc tagatggctt cgtacccctg ccatcaacac
      841 gcgtctgcgt tcgaccaggc tgcgcgttct cgcggccata acaaccgacg tacggcgttg
      901 cgccctcgcc ggcaacaaaa agccacggaa gtccgcctgg agcagaaaat gcccacgcta
      961 ctgcgggttt atatagacgg tccccacggg atggggaaaa ccaccaccac gcaactgctg
     1021 gtggccctgg gttcgcgcga cgatatcgtc tacgtacccg agccgatgac ttactggcgg
     1081 gtgttggggg cttccgagac aatcgcgaac atctacacca cacaacaccg cctcgaccag
     1141 ggtgagatat cggccgggga cgcggcggtg gtaatgacaa gcgcccagat aacaatgggc
     1201 atgccttatg ccgtgaccga cgccgttctg gctcctcata tcggggggga ggctgggagc
     1261 tcacatgccc cgcccccggc cctcaccctc atcttcgacc gccatcccat cgccgccctc
     1321 ctgtgctacc cggccgcgcg ataccttatg ggcagcatga ccccccaggc cgtgctggcg
     1381 ttcgtggccc tcatcccgcc gaccttgccc ggcacaaaca tcgtgttggg ggcccttccg
     1441 gaggacagac acatcgaccg cctggccaaa cgccagcgcc ccggcgagcg gcttgacctg
     1501 gctatgctgg ccgcgattcg ccgcgtttat gggctgcttg ccaatacggt gcggtatctg
     1561 cagggcggcg ggtcgtggcg ggaggattgg ggacagcttt cgggggcggc cgtgccgccc
     1621 cagggtgccg agccccagag caacgcgggc ccacgacccc atatcgggga cacgttattt
     1681 accctgtttc gggcccccga gttgctggcc cccaacggcg acctgtataa cgtgtttgcc
     1741 tgggctttgg acgtcttggc caaacgcctc cgtcccatgc acgtctttat cctggattac
     1801 gaccaatcgc ccgccggctg ccgggacgcc ctgctgcaac ttacctccgg gatggtccag
     1861 acccacgtca ccaccccagg ctccataccg acgatctgcg acctggcgcg cacgtttgcc
     1921 cgggagatgg gggaggctaa cgaatgcagt ggaggaggtt ctggaaggag gatatgcatg
     1981 aaaaagcctg aactcaccgc gacgtccgtc gagaagtttc tgatcgaaaa gttcgacagc
     2041 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     2101 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     2161 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     2221 gagttcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     2281 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     2341 atcgctgcgg ccgatctcag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     2401 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     2461 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     2521 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     2581 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     2641 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     2701 atggagcagc agacgcgcta cttcgagcgg aggcacccgg agcttgcagg atcgccgcgg
     2761 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     2821 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     2881 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     2941 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     3001 tagggtaccg agctcgaatt tggctttcgt gaccgggctt caaacaatga tgtgcgatgg
     3061 tgtggttccc ggttggcgga gtctttgtct actttggttg tctgtcgcag gtcggtagac
     3121 cgcaaatgag caactgatgg attgttgcca gcgatactat aattcacatg gatggtcttt
     3181 gtcgatcagt agctagtgag agagagagaa catctatcca caatgtcgag tgtctattag
     3241 acatactccg agaataaagt caactgtgtc tgtgatctaa agatcgattc ggcagtcgag
     3301 tagcgtataa caactccgag taccagcgaa agcacgtcgt gacaggagca gggctttgcc
     3361 aactgcgcaa ccttgcttga atgaggatac acggggtgca acatggctgt actgatccat
     3421 cgcaaccaaa atttctgttt atagatcaag ctggtagatt ccaattactc cacctcttgc
     3481 gcttctccat gacatgtaag tgcacgtgga aaccataccc aaattgccta cagctgcgga
     3541 gcatgagcct atggcgatca gtctggtcat gttaaccagc ctgtgctctg acgttaatgc
     3601 agaatagaaa gccgcggttg caatgcaaat gatgatgcct ttgcagaaat ggcttgctcg
     3661 ctgactgata ccagtaacaa ctttgcttgg ccgtctagcg ctgttgattg tattcatcac
     3721 aacctcgtct ccctcctttg ggttgagctc tcgagatgtc gactgattta aatcatgaag
     3781 cttaaagttt aaacgcggcc gcggatctgc cggtctccct atagtgagtc gtattaattt
     3841 cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4501 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4921 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4981 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5161 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5221 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5281 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5341 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5401 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5461 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5521 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5581 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5641 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5701 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5821 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5881 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     5941 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6001 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     6061 atgcggcatc agagcagatt gtactgagag tgcaccatat ggacatattg tcgttagaac
     6121 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
//
