LOCUS       40924_27089        5702 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal transformation vector pKT312, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     New plasmids allowing positive-negative selection for fungal 
            transformation and efficient Cre-loxP mediated marker recycling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2018) Department of Applied Genetics and Cell 
            Biology, University of Natural Resources and Life Sciences Vienna 
            (BOKU), Konrad Lorenz Strasse 24, Tulln 3430, Austria
REFERENCE   3  (bases 1 to 5702)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2018) Department of Applied Genetics and Cell Biology, 
            University of Natural Resources and Life Sciences Vienna (BOKU), 
            Konrad Lorenz Strasse 24, Tulln 3430, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      62..808
                     /note="pki; Trichoderma reesei pyruvate kinase I promoter; 
                     accession CP016233"
                     /regulatory_class="promoter"
     regulatory      215..224
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             814..1941
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     CDS             1954..2493
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      2544..3273
                     /note="cbh2; Trichoderma reesei cellobiohydrolase II
                     terminator; accession CP016238"
                     /regulatory_class="terminator"
     promoter        complement(3340..3358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3616..4204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4378..5235)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5236..5340)
                     /label=AmpR promoter
     promoter        5686..5702
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gcaggatcct taattaatcg aattctaggc cgcataggcc atactagtac
       61 tcgagataac ggtgagacta gcggccggtc cccttatccc agctgttcca cgttggcctg
      121 cccctcagtt agcgctcaac tcaatgcccc tcactggcga ggcgagggca aggatggagg
      181 ggcagcatcg cctgagttgg agcaaagcgg ccgggccgcc atgggagcag cgaaccaacg
      241 gagggatgcc gtgctttgtc gtggctgctg tggccaatcc gggcccttgg ttggctcaca
      301 gagcgttgct gtgagaccat gagctattat tgctaggtac agtatagaga gaggagagag
      361 agagagagag agagagaggg gaaaaaaggt gaggttgaag tgagaaaaaa aaaaaaaaaa
      421 aaaaatccaa ccactgacgg ctgccggctc tgccaccccc ctccctccac cccagaccac
      481 ctgcacactc agcgcgcagc atcacctaat cttggctcgc cttcccgcag ctcaggttgt
      541 tttttttttc tctctccctc gtcgaagccg cccttgttcc cttatttatt tccctctcca
      601 tccttgtctg cctttggtcc atctgcccct ttgtctgcat ctcttttgca cgcatcgcct
      661 tatcgtcgtc tcttttttca ctcacgggag cttgacgaag acctgactcg tgagcctcac
      721 ctgctgattt ctctcccccc ctcccgaccg gcttgacttt tgtttctcct ccagtacctt
      781 atcgcgaagc cggaagaacc tcttaacctc tagatggctt cgtacccctg ccatcaacac
      841 gcgtctgcgt tcgaccaggc tgcgcgttct cgcggccata acaaccgacg tacggcgttg
      901 cgccctcgcc ggcaacaaaa agccacggaa gtccgcctgg agcagaaaat gcccacgcta
      961 ctgcgggttt atatagacgg tccccacggg atggggaaaa ccaccaccac gcaactgctg
     1021 gtggccctgg gttcgcgcga cgatatcgtc tacgtacccg agccgatgac ttactggcgg
     1081 gtgttggggg cttccgagac aatcgcgaac atctacacca cacaacaccg cctcgaccag
     1141 ggtgagatat cggccgggga cgcggcggtg gtaatgacaa gcgcccagat aacaatgggc
     1201 atgccttatg ccgtgaccga cgccgttctg gctcctcata tcggggggga ggctgggagc
     1261 tcacatgccc cgcccccggc cctcaccctc atcttcgacc gccatcccat cgccgccctc
     1321 ctgtgctacc cggccgcgcg ataccttatg ggcagcatga ccccccaggc cgtgctggcg
     1381 ttcgtggccc tcatcccgcc gaccttgccc ggcacaaaca tcgtgttggg ggcccttccg
     1441 gaggacagac acatcgaccg cctggccaaa cgccagcgcc ccggcgagcg gcttgacctg
     1501 gctatgctgg ccgcgattcg ccgcgtttat gggctgcttg ccaatacggt gcggtatctg
     1561 cagggcggcg ggtcgtggcg ggaggattgg ggacagcttt cgggggcggc cgtgccgccc
     1621 cagggtgccg agccccagag caacgcgggc ccacgacccc atatcgggga cacgttattt
     1681 accctgtttc gggcccccga gttgctggcc cccaacggcg acctgtataa cgtgtttgcc
     1741 tgggctttgg acgtcttggc caaacgcctc cgtcccatgc acgtctttat cctggattac
     1801 gaccaatcgc ccgccggctg ccgggacgcc ctgctgcaac ttacctccgg gatggtccag
     1861 acccacgtca ccaccccagg ctccataccg acgatctgcg acctggcgcg cacgtttgcc
     1921 cgggagatgg gggaggctaa cgaatggatc catatgacca ctcttgacga cacggcttac
     1981 cggtaccgca ccagtgtccc gggggacgcc gaggccatcg aggcactgga tgggtccttc
     2041 accaccgaca ccgtcttccg cgtcaccgcc accggggacg gcttcaccct gcgggaggtg
     2101 ccggtggacc cgcccctgac caaggtgttc cccgacgacg aatcggacga cgaatcggac
     2161 gacggggagg acggcgaccc ggactcccgg acgttcgtcg cgtacgggga cgacggcgac
     2221 ctggcgggct tcgtggtcat ctcgtactcg gcgtggaacc gccggctgac cgtcgaggac
     2281 atcgaggtcg ccccggagca ccgggggcac ggggtcgggc gcgcgttgat ggggctcgcg
     2341 acggagttcg ccggcgagcg gggcgccggg cacctctggc tggaggtcac caacgtcaac
     2401 gcaccggcga tccacgcgta ccggcggatg gggttcaccc tctgcggcct ggacaccgcc
     2461 ctgtacgacg gcaccgcctc ggacggcgag cggcaggcgc tctacatgag catgccctgc
     2521 ccctgagcgg cctgcaggaa tttggctttc gtgaccgggc ttcaaacaat gatgtgcgat
     2581 ggtgtggttc ccggttggcg gagtctttgt ctactttggt tgtctgtcgc aggtcggtag
     2641 accgcaaatg agcaactgat ggattgttgc cagcgatact ataattcaca tggatggtct
     2701 ttgtcgatca gtagctagtg agagagagag aacatctatc cacaatgtcg agtgtctatt
     2761 agacatactc cgagaataaa gtcaactgtg tctgtgatct aaagatcgat tcggcagtcg
     2821 agtagcgtat aacaactccg agtaccagcg aaagcacgtc gtgacaggag cagggctttg
     2881 ccaactgcgc aaccttgctt gaatgaggat acacggggtg caacatggct gtactgatcc
     2941 atcgcaacca aaatttctgt ttatagatca agctggtaga ttccaattac tccacctctt
     3001 gcgcttctcc atgacatgta agtgcacgtg gaaaccatac ccaaattgcc tacagctgcg
     3061 gagcatgagc ctatggcgat cagtctggtc atgttaacca gcctgtgctc tgacgttaat
     3121 gcagaataga aagccgcggt tgcaatgcaa atgatgatgc ctttgcagaa atggcttgct
     3181 cgctgactga taccagtaac aactttgctt ggccgtctag cgctgttgat tgtattcatc
     3241 acaacctcgt ctccctcctt tgggttgagc tctcgagatg tcgactgatt taaatcatga
     3301 agcttaaagt ttaaacgcgg ccgcggatct gccggtctcc ctatagtgag tcgtattaat
     3361 ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3421 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4021 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     4081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4501 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4681 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4861 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4981 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5041 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5101 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5161 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5221 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5281 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5341 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     5401 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     5461 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     5521 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     5581 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
     5641 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
     5701 ta
//
