LOCUS       40924_27074        5702 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal transformation vector pKT304, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     New plasmids allowing positive-negative selection for fungal 
            transformation and efficient Cre-loxP mediated marker recycling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2018) Department of Applied Genetics and Cell 
            Biology, University of Natural Resources and Life Sciences Vienna 
            (BOKU), Konrad Lorenz Strasse 24, Tulln 3430, Austria
REFERENCE   3  (bases 1 to 5702)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2018) Department of Applied Genetics and Cell Biology, 
            University of Natural Resources and Life Sciences Vienna (BOKU), 
            Konrad Lorenz Strasse 24, Tulln 3430, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3180..4307
                     /codon_start=1
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
                     /translation="MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQKATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGAAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             4320..4859
                     /codon_start=1
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGER"
     promoter        complement(join(5701..5702,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccgc ggccgcgttt aaactttaag
     2401 cttcatgatt taaatcagtc gacatctcga gataacggtg agactagcgg ccggtcccct
     2461 tatcccagct gttccacgtt ggcctgcccc tcagttagcg ctcaactcaa tgcccctcac
     2521 tggcgaggcg agggcaagga tggaggggca gcatcgcctg agttggagca aagcggccgg
     2581 gccgccatgg gagcagcgaa ccaacggagg gatgccgtgc tttgtcgtgg ctgctgtggc
     2641 caatccgggc ccttggttgg ctcacagagc gttgctgtga gaccatgagc tattattgct
     2701 aggtacagta tagagagagg agagagagag agagagagag agaggggaaa aaaggtgagg
     2761 ttgaagtgag aaaaaaaaaa aaaaaaaaaa atccaaccac tgacggctgc cggctctgcc
     2821 acccccctcc ctccacccca gaccacctgc acactcagcg cgcagcatca cctaatcttg
     2881 gctcgccttc ccgcagctca ggttgttttt tttttctctc tccctcgtcg aagccgccct
     2941 tgttccctta tttatttccc tctccatcct tgtctgcctt tggtccatct gcccctttgt
     3001 ctgcatctct tttgcacgca tcgccttatc gtcgtctctt ttttcactca cgggagcttg
     3061 acgaagacct gactcgtgag cctcacctgc tgatttctct ccccccctcc cgaccggctt
     3121 gacttttgtt tctcctccag taccttatcg cgaagccgga agaacctctt aacctctaga
     3181 tggcttcgta cccctgccat caacacgcgt ctgcgttcga ccaggctgcg cgttctcgcg
     3241 gccataacaa ccgacgtacg gcgttgcgcc ctcgccggca acaaaaagcc acggaagtcc
     3301 gcctggagca gaaaatgccc acgctactgc gggtttatat agacggtccc cacgggatgg
     3361 ggaaaaccac caccacgcaa ctgctggtgg ccctgggttc gcgcgacgat atcgtctacg
     3421 tacccgagcc gatgacttac tggcgggtgt tgggggcttc cgagacaatc gcgaacatct
     3481 acaccacaca acaccgcctc gaccagggtg agatatcggc cggggacgcg gcggtggtaa
     3541 tgacaagcgc ccagataaca atgggcatgc cttatgccgt gaccgacgcc gttctggctc
     3601 ctcatatcgg gggggaggct gggagctcac atgccccgcc cccggccctc accctcatct
     3661 tcgaccgcca tcccatcgcc gccctcctgt gctacccggc cgcgcgatac cttatgggca
     3721 gcatgacccc ccaggccgtg ctggcgttcg tggccctcat cccgccgacc ttgcccggca
     3781 caaacatcgt gttgggggcc cttccggagg acagacacat cgaccgcctg gccaaacgcc
     3841 agcgccccgg cgagcggctt gacctggcta tgctggccgc gattcgccgc gtttatgggc
     3901 tgcttgccaa tacggtgcgg tatctgcagg gcggcgggtc gtggcgggag gattggggac
     3961 agctttcggg ggcggccgtg ccgccccagg gtgccgagcc ccagagcaac gcgggcccac
     4021 gaccccatat cggggacacg ttatttaccc tgtttcgggc ccccgagttg ctggccccca
     4081 acggcgacct gtataacgtg tttgcctggg ctttggacgt cttggccaaa cgcctccgtc
     4141 ccatgcacgt ctttatcctg gattacgacc aatcgcccgc cggctgccgg gacgccctgc
     4201 tgcaacttac ctccgggatg gtccagaccc acgtcaccac cccaggctcc ataccgacga
     4261 tctgcgacct ggcgcgcacg tttgcccggg agatggggga ggctaacgaa tggatccata
     4321 tgaccactct tgacgacacg gcttaccggt accgcaccag tgtcccgggg gacgccgagg
     4381 ccatcgaggc actggatggg tccttcacca ccgacaccgt cttccgcgtc accgccaccg
     4441 gggacggctt caccctgcgg gaggtgccgg tggacccgcc cctgaccaag gtgttccccg
     4501 acgacgaatc ggacgacgaa tcggacgacg gggaggacgg cgacccggac tcccggacgt
     4561 tcgtcgcgta cggggacgac ggcgacctgg cgggcttcgt ggtcatctcg tactcggcgt
     4621 ggaaccgccg gctgaccgtc gaggacatcg aggtcgcccc ggagcaccgg gggcacgggg
     4681 tcgggcgcgc gttgatgggg ctcgcgacgg agttcgccgg cgagcggggc gccgggcacc
     4741 tctggctgga ggtcaccaac gtcaacgcac cggcgatcca cgcgtaccgg cggatggggt
     4801 tcaccctctg cggcctggac accgccctgt acgacggcac cgcctcggac ggcgagcggc
     4861 aggcgctcta catgagcatg ccctgcccct gagcggcctg caggaatttg gctttcgtga
     4921 ccgggcttca aacaatgatg tgcgatggtg tggttcccgg ttggcggagt ctttgtctac
     4981 tttggttgtc tgtcgcaggt cggtagaccg caaatgagca actgatggat tgttgccagc
     5041 gatactataa ttcacatgga tggtctttgt cgatcagtag ctagtgagag agagagaaca
     5101 tctatccaca atgtcgagtg tctattagac atactccgag aataaagtca actgtgtctg
     5161 tgatctaaag atcgattcgg cagtcgagta gcgtataaca actccgagta ccagcgaaag
     5221 cacgtcgtga caggagcagg gctttgccaa ctgcgcaacc ttgcttgaat gaggatacac
     5281 ggggtgcaac atggctgtac tgatccatcg caaccaaaat ttctgtttat agatcaagct
     5341 ggtagattcc aattactcca cctcttgcgc ttctccatga catgtaagtg cacgtggaaa
     5401 ccatacccaa attgcctaca gctgcggagc atgagcctat ggcgatcagt ctggtcatgt
     5461 taaccagcct gtgctctgac gttaatgcag aatagaaagc cgcggttgca atgcaaatga
     5521 tgatgccttt gcagaaatgg cttgctcgct gactgatacc agtaacaact ttgcttggcc
     5581 gtctagcgct gttgattgta ttcatcacaa cctcgtctcc ctcctttggg ttgagctctc
     5641 gagtactagt atggcctatg cggcctagaa ttcgattaat taaggatcct gcggccgcgt
     5701 tc
//
