LOCUS       40924_27049        9277 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKT240tet2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9277)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9277)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             86..1273
                     /label=TcR
                     /note="tetracycline efflux protein"
     rep_origin      complement(2747..3141)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(3168..3452)
                     /codon_start=1
                     /note="unnamed protein product; mobC"
                     /protein_id="SJL88502.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     oriT            3483..3570
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4399..4812
                     /codon_start=1
                     /note="unnamed protein product; mobB"
                     /protein_id="SJL88504.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     CDS             4809..5777
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             6055..6261
                     /codon_start=1
                     /note="unnamed protein product; rpsF"
                     /protein_id="SJL88506.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             6291..7127
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             7117..7965
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     1861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     1921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     1981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2041 gaccacgctg atgagcttta ccgcagggcc gcgatcaagc aggtgcgaca gacgtcatac
     2101 tagatatcaa gcgacttctc ctatcccctg ggaacacatc aatctcaccg gagaatatcg
     2161 ctggccaaag ccttagcgta ggattccgcc ccttcccgca aacgacccca aacaggaaac
     2221 gcagctgaaa cgggaagctc aacacccact gacgcatggg ttgttcaggc agtacttcat
     2281 caaccagcaa ggcggcactt tcggccatcc gccgcgcccc acagctcggg cagaaaccgc
     2341 gacgcttaca gctgaaagcg accaggtgct cggcgtggca agactcgcag cgaacccgta
     2401 gaaagccatg ctccagccgc ccgcattgga gaaattcttc aaattcccgt tgcacatagc
     2461 ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa
     2521 cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat
     2581 cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt
     2641 tacatacagt ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt
     2701 gctagacatt gccagccagt gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat
     2761 aactgtcacg cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca
     2821 aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt
     2881 atctaagaat aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg
     2941 cccaatatgc ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc
     3001 gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac
     3061 cccgcagaaa tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag
     3121 ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gcccggctca
     3181 tccttctggc gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca
     3241 tccattgccg ccatgagccg atcctccggc cactcgctgc tgttcacctt ggccaaaatc
     3301 atggccccca ccagcacctt gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt
     3361 gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag cttggccagc
     3421 cgatccgccg ccttgttgct ccccttaacc atcttgacac cccattgtta atgtgctgtc
     3481 tcgtaggcta tcatggaggc acagcggcgg caatcccgac cctactttgt aggggagggc
     3541 gcacttaccg gtttctcttc gagaaactgg cctaacggcc acccttcggg cggtgcgctc
     3601 tccgagggcc attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt
     3661 atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg gccaaggccg
     3721 actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg cacgccgaat
     3781 ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct gccgacctgt
     3841 atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga
     3901 ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgacc ggtgccgagc
     3961 gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac tgccacctga
     4021 tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt
     4081 acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc aagcccaagg
     4141 catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca ttagagcggg
     4201 ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc gagcgcctgc
     4261 ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc cgcaccgacc
     4321 gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac ttacaggaat
     4381 accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag aggcatcaac
     4441 gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc cgacgaagcc
     4501 cggcaggtca tgagccagac ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa
     4561 gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg ggaggtagcc
     4621 gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag
     4681 ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct gatcgcatcg
     4741 ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg gctgcgcttg
     4801 gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc aaggccatgg
     4861 gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac cggccagatg atgaaccggg
     4921 aatggtcagc cgccgaagtg ctccagaaca cgccatggct caagcggatg aatgcccagg
     4981 gcaatgacgt gtatatcagg cccgccgagc aggagcggca tggtctggtg ctggtggacg
     5041 acctcagcga gtttgacctg gatgacatga aagccgaggg ccgggagcct gccctggtag
     5101 tggaaaccag cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg
     5161 aacttcgggg gcagattgcc cggacgctgg ccagcgagta cgacgccgac ccggccagcg
     5221 ccgacagccg ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca
     5281 cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag accgccaccg
     5341 ctggcccggc gctggtgcag caggctggcc agcagatcga gcaggcccag cggcagcagg
     5401 agaaggcccg caggctggcc agcctcgaac tgcccgagcg gcagcttagc cgccaccggc
     5461 gcacggcgct ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg
     5521 acctcagcaa gtgcgacttt atcgccgcgc agaagctggc cagccggggc cgcagtgccg
     5581 aggaaatcgg caaggccatg gccgaggcca gcccagcgct ggcagagcgc aagcccggcc
     5641 acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc
     5701 ttgcgcgggc cgagctggca cgggcaccgg caccccgcca gcgaggcatg gacaggggcg
     5761 ggccagattt cagcatgtag tgcttgcgtt ggtactcacg cctgttatac tatgagtact
     5821 cacgcacaga agggggtttt atggaatacg aaaaaagcgc ttcagggtcg gtctacctga
     5881 tcaaaagtga caagggctat tggttgcccg gtggctttgg ttatacgtca aacaaggccg
     5941 aggctggccg cttttcagtc gctgatatgg ccagccttaa ccttgacggc tgcaccttgt
     6001 ccttgttccg cgaagacaag cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa
     6061 gaccaaaagg acaagcagac cggcgacctg ctggccagcc ctgacgctgt acgccaagcg
     6121 cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac
     6181 gacgaatacg aggcgctgcg cgagtgcctg gaagaactca gagcggcgca gggcgggggt
     6241 agtgaccccg ccagcgccta accaccaact gcctgcaaag gaggcaatca atggctaccc
     6301 ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg gactacgttt
     6361 tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt gccggtaaat
     6421 ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg
     6481 gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc accgccattc
     6541 atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg
     6601 acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc ccggagtggt
     6661 tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac acgctgcgcc
     6721 ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg
     6781 aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg
     6841 cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg gtactggtcg
     6901 ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc gaggaatggg
     6961 gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag gccaactatg
     7021 gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc aagcccgccg
     7081 tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac
     7141 agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct gttccgtgcc
     7201 ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta cggcgacggc
     7261 aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg catcctgcaa
     7321 gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc caagaccgaa
     7381 ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac cgctgaatgc
     7441 catgtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt
     7501 ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt atccatcatc
     7561 gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc cagcgacgag
     7621 gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt catgggtggc
     7681 ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga aaccgcccgc
     7741 ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa ggcttccata
     7801 gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc
     7861 cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac ggtaaccgag
     7921 ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc ccccccactc
     7981 tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct gctaattggt
     8041 aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa aattgcctac
     8101 tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag atgtatgctc
     8161 ttctgctcct cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     8221 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     8281 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     8341 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
     8401 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt
     8461 tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag
     8521 gcagacctca gcgctaaccg tttttatcag gctctgggag gcagaataaa tgatcatatc
     8581 gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat ttgagaagca
     8641 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
     8701 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca
     8761 ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac
     8821 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     8881 gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca
     8941 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     9001 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     9061 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     9121 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     9181 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     9241 atatcaccag ctcaccgtct ttcattgcca tacggaa
//
