LOCUS       V014085                 7139 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014085
VERSION     V014085
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        91..121
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     153..169
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(182..238)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(239..255)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        729..833
                     /label="AmpR promoter"
     CDS             834..1691
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1865..2453
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2989..3636)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             4167..4898
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      181 caagcttgca tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac
      241 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      301 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      361 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      421 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
      481 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      541 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      601 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
      661 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      721 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      781 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      841 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      901 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      961 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1021 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1081 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1141 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1201 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1261 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1321 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1381 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1441 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1501 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1561 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1621 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1681 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1741 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1801 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1861 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1921 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1981 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2041 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2101 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2161 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2221 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2281 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2341 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2401 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2461 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2521 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2581 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2641 caatacgcaa accgttggcc gattcattaa tgcagctgat ttcacttttt gcgagcgagg
     2701 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     2761 gcagctgatt tcactttttg cattctacaa actgcataac tcatatgtaa atcgctcctt
     2821 tttaggtggc acaaatgtga ggcattttcg ctctttccgg cgaggctagt tacccttaag
     2881 ttattggtat gactggtttt aagcgccaaa aaagttgctt tttcgtacct attaatgtat
     2941 cgttagaaaa ccgactgtaa aaagtacagt cggcattatc tcatattata aaagccagtc
     3001 attaggccta tctgacaatt cctgaataga gttcataaac aatcctgcat gataaccatc
     3061 acaaacagaa tgatgtacct gtaaagatag cggtaaatat attgaattac ctttattaat
     3121 gaattttcct gctgtaataa tgggtagaag gtaattacta ttattattga tatttaagtt
     3181 aaacccagta aatgaagtcc atggaataat agaaagagaa aaagcatttt caggtatagg
     3241 tgttttggga aacaatttcc ccgaaccatt atatttctct acatcagaaa ggtataaatc
     3301 ataaaactct ttgaagtcat tctttacagg agtccaaata ccagagaatg ttttagatac
     3361 accatcaaaa attgtataaa gtggctctaa cttatcccaa taacctaact ctccgtcgct
     3421 attgtaacca gttctaaaag ctgtatttga gtttatcacc cttgtcacta agaaaataaa
     3481 tgcagggtaa aatttatatc cttcttgttt tatgtttcgg tataaaacac taatatcaat
     3541 ttctgtggtt atactaaaag tcgtttgttg gttcaaataa tgattaaata tctcttttct
     3601 cttccaattg tctaaatcaa ttttattaaa gttcatttga tatgcctcct aaatttttat
     3661 ctaaagtgaa tttaggaggc ttacttgtct gctttcttca ttagaatcaa tcctttttta
     3721 aaagtcaata ttactgtaac ataaatatat attttaaaaa tatcccactt tatccaattt
     3781 tcgtttgttg aactaatggg tgctttagtt gaagaataaa agaccacatt aaaaaatgtg
     3841 gtcttttgtg tttttttaaa ggatttgagc gtagcgaaaa atccttttct ttcttatctt
     3901 gatactaagg gtaactattg ccgggataga ctgtaacatt ctcacgcata aaatcccctt
     3961 tcattttcta atgtaaatct attaccttat tattaattca attcgctcat aattaatcct
     4021 ttttcttatt acgcaaaatg gcccgattta agcacaccct ttattccgtt aatgcgccat
     4081 gacagccatg ataattacta atactaggag aagttaataa atacgtaacc aacatgatta
     4141 acaattatta gaggtcatcg ttcaaaatga acgagaaaaa tataaaacac agtcaaaact
     4201 ttattacttc aaaacataat atagataaaa taatgacaaa tataagatta aatgaacatg
     4261 ataatatctt tgaaatcggc tcaggaaaag gccattttac ccttgaatta gtaaagaggt
     4321 gtaatttcgt aactgccatt gaaatagacc ataaattatg caaaactaca gaaaataaac
     4381 ttgttgatca cgataatttc caagttttaa acaaggatat attgcagttt aaatttccta
     4441 aaaaccaatc ctataaaata tatggtaata taccttataa cataagtacg gatataatac
     4501 gcaaaattgt ttttgatagt atagctaatg agatttattt aatcgtggaa tacgggtttg
     4561 ctaaaagatt attaaataca aaacgctcat tggcattact tttaatggca gaagttgata
     4621 tttctatatt aagtatggtt ccaagagaat attttcatcc taaacctaaa gtgaatagct
     4681 cacttatcag attaagtaga aaaaaatcaa gaatatcaca caaagataaa caaaagtata
     4741 attatttcgt tatgaaatgg gttaacaaag aatacaagaa aatatttaca aaaaatcaat
     4801 ttaacaattc cttaaaacat gcaggaattg acgatttaaa caatattagc tttgaacaat
     4861 tcttatctct tttcaatagc tataaattat ttaataagta agttaaggga tgcataaact
     4921 gcatccctta acttgttttt cgtgtgccta ttttttgtga atcgattatg tcttttgcga
     4981 gtcggcttaa accagttttc gttggtgcga aaaaagagtg tcttgtgaca ctcttaaatt
     5041 caaaatctat cggtcagatt tataccgatt tgattttata tattcttgaa taacatacgc
     5101 cgagttatca cataaaagcg ggaaccaatc atcaaattta aacttcattg cataatccat
     5161 taaactctta aattctacga ttccttgttc atcaataaac tcaatcattt ctttaattaa
     5221 tttatatcta tctgttgttg ttttctttaa taattcatca acatctacac cgccataaac
     5281 tatcatatct tctttttgat atttaaattt attaggatcg tccatgtgaa gcatatatct
     5341 cacaagacct ttcacacttc ctgcaatctg cggaatagtc gcattcaatt cttctgtaat
     5401 tatttttatc tgttcataag atttattacc ctcatacatc actagaatat gataatgctc
     5461 ttttttcatc ctatcttctg tatcagtatc cctatcatgt aatggagaca ctacaaattg
     5521 aatgtgtaac tcttttaaat actctaacca ctcggctttt gctgattctg gatataaaac
     5581 aaatgtccaa ttacgtcctc ttgaattttt cttgttttca gtttctttta ttacattttc
     5641 gctcatgata taataacggt gctaatacat ttaacaaaat ttagtcatag ataggcagca
     5701 tgccagtgct gtctatcttt ttttgtttaa aatgcaccgt attcctcctt tgcatatttt
     5761 tttattagaa taccggttgc atctgatttg ctaatattat atttttcttt gattctattt
     5821 aatatctcat tttcttctgt tgtaagtctt aaagtaacag caactttttt ctcttctttt
     5881 ctatctacaa ccatcactgt acctcccaac atctgttttt ttcactttaa cataaaaaac
     5941 aaccttttaa cattaaaaac ccaatattta tttatttgtt tggacaatgg acaatggaca
     6001 cctagggggg aggtcgtagt acccccctat gttttctccc ctaaataacc ccaaaaatct
     6061 aagaaaaaaa gacctcaaaa aggtctttaa ttaacatctc aaatttcgca tttattccaa
     6121 tttccttttt gcgtgtgatg cgctgcgtcc attaaaaatc ctagagcttt gcaaccgaaa
     6181 gttaatagct gtcgctacta ctttcgctta cgctctaagt atattttaag gactgtcaca
     6241 cgcaaaaagt tttctcggca taaaagtacc tctacatctc taaatcgtct gtacgctgtt
     6301 tctcacgctt tctatcgacc ttctggacat tatcctgtac aacatccata aactgtccca
     6361 cacgctcgaa tttggaatca ttaaagaatt tctctttaag cctattaaac cctttctcaa
     6421 acccagggaa attcgccctc gcagcacgat ataaagtcac tgtactatct tgaaatttct
     6481 ctgatacatt caactgctca ttcaaactat cattctctcg ctttaattta ttaacctctt
     6541 tacttttttc gtgatacccc tctttccatg tattcactac ttctttcaaa ctctctctac
     6601 gtttttttaa ttcttgattt tctgtgtaat agtctgtgct cttaatattt tcgtaatcat
     6661 caacaatccg ttcgaagaga ttgtttcttg caggcgttca aattcatcag cagttaatat
     6721 ctttctacca gtctcttcac gtccagagaa caaacctgta cgctcatttt cataatcaaa
     6781 gggtttcgta gacctcatat gctctattcc actctgtaac tgcttatttg ccttctgtaa
     6841 ctcatcctta acttcttgca gttcctgttt atgaaataca gtatctttct tgtactgatc
     6901 cagttgctca aaaaaatctc ggtcagatgt tactagcaac tcatttacaa gaacagcatc
     6961 tttcctcgtt tttcttgtac ctgttttttg tgattcaata atttctttga cacgttcgtt
     7021 gtaatcaata tttttatcat ttttcaaatc ataattttca cgtgttcgct catggtcaat
     7081 atcatcattc gttctacttt ttcgctctct ttgattatga aattgcatgc cttttagtc
//
