LOCUS       V005187                 5368 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005187
VERSION     V005187
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Saijo M, Qing T, Niikura M, Maeda A, Ikegami T, Sakai K, Prehaud C,
            Kurane I, Morikawa S.
  TITLE     Immunofluorescence technique using HeLa cells expressing recombinant
            nucleoprotein for detection of immunoglobulin G antibodies to
            Crimean-Congo hemorrhagic fever virus
  JOURNAL   J. Clin. Microbiol. 40 (2), 372-375 (2002)
   PUBMED   11825944
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Sakai K.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2001) AIDS Research Center, National Institute of
            Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011,
            Japan
REFERENCE   3  (bases 1 to 5368)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5368)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Clin.
            Microbiol."; date: "2002"; volume: "40"; issue: "2"; pages:
            "372-375"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-JUL-2001) AIDS Research Center, National Institute of Infectious
            Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..1192
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     misc_feature    1194..1243
                     /label="multiple cloning sites"
                     /note="multiple cloning sites"
     polyA_signal    complement(1239..1373)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1798..1818)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1948..2013)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(2262..2681)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     promoter        complement(2729..3058)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    3209..3349
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3535..4123)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4297..5154)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5155..5259)
                     /label="AmpR promoter"
ORIGIN
        1 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
       61 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      121 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      181 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      241 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      301 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      361 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      421 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      481 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      541 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
      601 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
      661 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
      721 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
      781 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
      841 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
      901 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
      961 cacctcgatt agttctcgat cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     1021 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     1081 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     1141 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattca
     1201 agcttgcctc gaggccgcgg ccgcggtcga ctctagagga tccagacatg ataagataca
     1261 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1321 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1381 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1441 agtaaaacct ctacaaatgt ggtatggctg attatgatct ctagtcaagg cactatacat
     1501 caaatattcc ttattaaccc ctttacaaat taaaaagcta aaggtacaca atttttgagc
     1561 atagttatta atagcagaca ctctatgcct gtgtggagta agaaaaaaca gtatgttatg
     1621 attataactg ttatgcctac ttataaaggt tacagaatat ttttccataa ttttcttgta
     1681 tagcagtgca gctttttcct ttgtggtgta aatagcaaag caagcaagag ttctattact
     1741 aaacacagca tgactcaaaa aacttagcaa ttctgaagga aagtccttgg ggtcttctac
     1801 ctttctcttc ttttttggag gagtagaatg ttgagagtca gcagtagcct catcatcact
     1861 agatggcatt tcttctgagc aaaacaggtt ttcctcatta aaggcattcc accactgctc
     1921 ccattcatca gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt
     1981 aacacattat acacttaaaa attttatatt taccttagag ctttaaatct ctgtaggtag
     2041 tttgtccaat tatgtcacac cacagaagta aggttccttc acaaagatcc gggaccaaag
     2101 cggccatcgt gcctccccac tcctgcagtt cgggggcatg gatgcgcgga tagccgctgc
     2161 tggtttcctg gatgccgacg gatttgcact gccggtagaa ctccgcgagg tcgtccagcc
     2221 tcaggcagca gccaagctag cttggtatgg taaaactttt aatttcgggt atatttgagt
     2281 ggaatgagtt cttcaatcgt agttttgact aacttgccat tcatttctat taacacaaaa
     2341 caatctggtg catagtctga aatcaactcc ctacacatac cacaaggact taccactcga
     2401 atacttctat ctacttcgtc agaataaggg tgtctaacag ctacaatcgt gtcaaaatcc
     2461 ttttgtccat tcgaaactgc actaccaatg gcaatggctt ctgcacaaac agttactcgt
     2521 cctatatacg cttcaatatg tactgccgaa atgatttctc ctgttttcgt acgaattgcc
     2581 gctcccacat gatgtttatt atcctcataa agcattgtaa tcttctctgt cgctacttct
     2641 actaattcta gatcttgttg agaaatgtta aatgttttca tatattcttc ttcctccgat
     2701 ttctgcaaaa atgaatccaa gctagctttt tgcaaaagcc taggcctcca aaaaagcctc
     2761 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     2821 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     2881 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     2941 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     3001 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     3061 tccacagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3121 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     3181 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     3241 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     3301 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     3361 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3421 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3481 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3541 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3601 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3661 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3721 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3781 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3841 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3901 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3961 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4021 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4081 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4141 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4201 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4261 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4321 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4381 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4441 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4501 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4561 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     4621 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4681 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4741 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4801 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4861 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     4921 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4981 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5041 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5101 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5161 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5221 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5281 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     5341 gaggcccttt cgtcttcaag aattaatt
//
