LOCUS       40924_26984        8959 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKRCPN1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Monson RE, Drew AM, Smith D, Matilla M, Roberts K, Richardson E, 
            Williamson N, Ramsay J, Welch M, Salmond GPC.
  TITLE     A plasmid-transposon hybrid mutagenesis system effective in a broad 
            range of Enterobacteria
  JOURNAL   Front Microbiol (2016) In press
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Monson RE, Drew AM, Smith D, Matilla M, Roberts K, Richardson E, 
            Williamson N, Ramsay J, Welch M, Salmond GPC.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2015) Biochemistry, University of Cambridge, 
            Tennis Court Road, Cambridge CB2 1QW, United Kingdom
REFERENCE   3  (bases 1 to 8959)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8959)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2015) Biochemistry, University of Cambridge, Tennis Court 
            Road, Cambridge CB2 1QW, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(106..1293)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        complement(1341..1369)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             1747..3174
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAAQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    3186..3204
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     CDS             3443..6514
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGKLRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     rep_origin      complement(6593..6981)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             7213..8025
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(8390..8408)
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     oriT            complement(8647..8756)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 tagttcaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
       61 ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
      121 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat
      181 ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg
      241 tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg
      301 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
      361 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
      421 gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
      481 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
      541 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
      601 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
      661 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
      721 cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
      781 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
      841 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
      901 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
      961 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atccacagga cgggtgtggt
     1021 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg
     1081 gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata
     1141 tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa
     1201 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
     1261 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt
     1321 taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt
     1381 acaacttata tcgtatgggg ctgacttcag gtgctacatt tgaagagata aattgcactg
     1441 aaatctagaa tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt
     1501 gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg
     1561 tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt
     1621 attcggctgc aactttgtca tgcttgatac tttatcactg ataaacataa tatgtccacc
     1681 aacttatcag tgataaagaa tccgcgcgtt caatcggacc agcggaggct agtccggagg
     1741 ccacatatga taacttctgc tcttcatcgt gcggccgact gggctaaatc tgtgttctct
     1801 tcggcggcgc tgggtgatcc tcgccgtact gcccgcttgg ttaacgtcgc cgcccaattg
     1861 gcaaaatatt ctggtaaatc aataaccatc tcatcagagg gtagtaaagc cgcccaggaa
     1921 ggcgcttacc gatttatccg caatcccaac gtttctgccg aggcgatcag aaaggctggc
     1981 gccatgcaaa cagtcaagtt ggctcaggag tttcccgaac tgctggccat tgaggacacc
     2041 acctctttga gttatcgcca ccaggtcgcc gaagagcttg gcaagctggg ctctattcag
     2101 gataaatccc gcggatggtg ggttcactcc gttctcttgc tcgaggccac cacattccgc
     2161 accgtaggat tactgcatca ggagtggtgg atgcgcccgg atgaccctgc cgatgcggat
     2221 gaaaaggaga gtggcaaatg gctggcagcg gccgcaacta gccggttacg catgggcagc
     2281 atgatgagca acgtgattgc ggtctgtgac cgcgaagccg atattcatgc ttatctgcag
     2341 gacaaactgg cgcataacga gcgcttcgtg gtgcgctcca agcacccacg caaggacgta
     2401 gagtctgggt tgtatctgta cgaccatctg aagaaccaac cggagttggg tggctatcag
     2461 atcagcattc cgcaaaaggg cgtggtggat aaacgcggta aacgtaaaaa tcgaccagcc
     2521 cgcaaggcga gcttgagcct gcgcagtggg cgcatcacgc taaaacaggg gaatatcacg
     2581 ctcaacgcgg tgctggccga ggagattaac ccgcccaagg gtgagacccc gttgaaatgg
     2641 ttgttgctga ccagcgaacc ggtcgagtcg ctagcccaag ccttgcgcgt catcgacatt
     2701 tatacccatc gctggcggat cgaggagttc cataaggcat ggaaaaccgg agcaggagcc
     2761 gagaggcaac gcatggagga gccggataat ctggagcgga tggtctcgat cctctcgttt
     2821 gttgcggtca ggctgttaca gctcagagaa agcttcacgc cgccgcaagc actcagggcg
     2881 caagggctgc taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc
     2941 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa
     3001 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     3061 aagcgaaccg gaattgccag ctggggcgcc ctctgggaag gttgggaagc cctgcaaagt
     3121 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatcc
     3181 gtcgactgtc tcttatacac atctcaacca tcatcgatga attcgagctc ggtaccagca
     3241 gcgattgatg caggagctgc gggcgcgatt tctggttcgg ccattgttaa aatcatcgag
     3301 caacatatta atgagccaga gaaaatgctg gcggcactga aagtttttgt acaaccgatg
     3361 aaagcggcga cgcgcagtta atcccacagc cgccagttcc gctggcggca ttttaacttt
     3421 ctttatcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca
     3481 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3541 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3601 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
     3661 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
     3721 gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc
     3781 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
     3841 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
     3901 gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga
     3961 cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag
     4021 tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
     4081 ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa
     4141 tgatgatttc agccgcgctg tactggaggc tgaagttcaa atgtgcggca agttgcgtga
     4201 ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc
     4261 gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg
     4321 tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
     4381 ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt
     4441 ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg
     4501 aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
     4561 ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta
     4621 tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga
     4681 agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg
     4741 gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag
     4801 tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta
     4861 tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga
     4921 caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt
     4981 cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc
     5041 gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata
     5101 ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga
     5161 tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt
     5221 tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac
     5281 gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc
     5341 cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct
     5401 gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt
     5461 cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg
     5521 gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg
     5581 gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc
     5641 cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg
     5701 taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
     5761 taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga
     5821 cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc
     5881 ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga
     5941 tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag
     6001 ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt
     6061 ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc
     6121 agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc
     6181 cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc
     6241 gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg
     6301 cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca
     6361 tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg
     6421 gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattacagc tgagcgccgg
     6481 tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca ggccatgtct
     6541 gcccgtattt cgcgtaagga aatccattat gtactatttc ggtacccccc catgtcagcc
     6601 gttaagtgtt cctgtgtcac tcaaaattgc tttgagaggc tctaagggct tctcagtgcg
     6661 ttacatccct ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat
     6721 attctttttt ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat
     6781 taattttatt gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag
     6841 ccatgagagc ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta
     6901 cgttaaacat gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt
     6961 tgaactgctg atcttcagat cctctacgcc ggacgcatcg tggccggggt tcgaaacttt
     7021 tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca
     7081 gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt
     7141 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     7201 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat
     7261 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     7321 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     7381 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     7441 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     7501 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca
     7561 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     7621 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     7681 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     7741 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat
     7801 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     7861 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     7921 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     7981 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     8041 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa
     8101 taattaatta accgagagct tggcacccag cctgcgcgag caggggaatt gatccggtgg
     8161 atgacctttt gaatgacctt taatagatta tattactaat taattgggga ccctagaggt
     8221 cccctttttt attttaaaaa ttttttcaca aaacggttta caagcataaa gcttgctcaa
     8281 tcaatcaccg gatccccggg aattctcatg tttgacagct tatcatcgat aagcttgcct
     8341 cgcgcgataa atctagagtc gacctgcagg catgcaagct tcagggttga gatgtgtata
     8401 agagacagtc gacctgcagg atcgatggcg cgccgacgtg ctcatagtcc acgacgcccg
     8461 tgattttgta gccctggccg acggccagca ggtaggccga caggctcatg ccggccgccg
     8521 ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca gtacaccttg ataggtgggc
     8581 tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg
     8641 cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
     8701 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
     8761 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
     8821 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
     8881 cagcggaaaa gcgctgcttc cctgctgttt tgtggcgcgc catcgatccg ccggaattcc
     8941 tgcagcccgg gggatccac
//
