LOCUS       V005193                 6067 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005193
VERSION     V005193
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Rossger K, Charpin-El Hamri G, Fussenegger M.
  TITLE     Reward-based hypertension control by a synthetic brain-dopamine
            interface
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 110 (45), 18150-18155 (2013)
   PUBMED   24127594
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Roessger K, Fussenegger M, Charpin-El-Hamri G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel,
            BS 4058, Switzerland
REFERENCE   3  (bases 1 to 6067)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6067)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2013"; volume: "110"; issue: "45"; pages:
            "18150-18155"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel, BS 4058,
            Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             656..1993
                     /gene="DRD1"
                     /label="D(1A) dopamine receptor"
                     /note="D(1A) dopamine receptor from Homo sapiens.
                     Accession#: P21728"
     CDS             2013..2729
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    2855..2976
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2983..3438)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3465..3569
                     /label="AmpR promoter"
     promoter        3571..3928
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3963..4754
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4989..5036
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5365..5953
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc ccaccatgag
      661 gactctgaac acctctgcca tggacgggac tgggctggtg gtggagaggg acttctctgt
      721 tcgtatcctc actgcctgtt tcctgtcgct gctcatcctg tccacgctcc tggggaacac
      781 gctggtctgt gctgccgtta tcaggttccg acacctgcgg tccaaggtga ccaacttctt
      841 tgtcatctcc ttggctgtgt cagatctctt ggtggccgtc ctggtcatgc cctggaaggc
      901 agtggctgag attgctggct tctggccctt tgggtccttc tgtaacatct gggtggcctt
      961 tgacatcatg tgctccactg catccatcct caacctctgt gtgatcagcg tggacaggta
     1021 ttgggctatc tccagccctt tccggtatga gagaaagatg acccccaagg cagccttcat
     1081 cctgatcagt gtggcatgga ccttgtctgt actcatctcc ttcatcccag tgcagctcag
     1141 ctggcacaag gcaaaaccca caagcccctc tgatggaaat gccacttccc tggctgagac
     1201 catagacaac tgtgactcca gcctcagcag gacatatgcc atctcatcct ctgtaataag
     1261 cttttacatc cctgtggcca tcatgattgt cacctacacc aggatctaca ggattgctca
     1321 gaaacaaata cggcgcattg cggccttgga gagggcagca gtccacgcca agaattgcca
     1381 gaccaccaca ggtaatggaa agcctgtcga atgttctcaa ccggaaagtt cttttaagat
     1441 gtccttcaaa agagaaacta aagtcctgaa gactctgtcg gtgatcatgg gtgtgtttgt
     1501 gtgctgttgg ctacctttct tcatcttgaa ctgcattttg cccttctgtg ggtctgggga
     1561 gacgcagccc ttctgcattg attccaacac ctttgacgtg tttgtgtggt ttgggtgggc
     1621 taattcatcc ttgaacccca tcatttatgc ctttaatgct gattttcgga aggcattttc
     1681 aaccctctta ggatgctaca gactttgccc tgcgacgaat aatgccatag agacggtgag
     1741 tatcaataac aatggggccg cgatgttttc cagccatcat gagccacgag gctccatctc
     1801 caaggagtgc aatctggttt acctgatccc acatgctgtg ggctcctctg aggacctgaa
     1861 aaaggaggag gcagctggca tcgccagacc cttggagaag ctgtccccag ccctatcagt
     1921 catattggac tatgacactg acgtctctct ggagaagatc caacccatca cacaaaacgg
     1981 tcagcaccca accggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     2041 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2101 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2161 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     2221 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2281 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2341 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2401 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2461 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2521 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2581 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     2641 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2701 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag
     2761 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2821 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2881 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2941 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     3001 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3061 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3121 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3181 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3241 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3301 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3361 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3421 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3481 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3541 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3601 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3661 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3721 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3781 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3841 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3901 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     3961 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4021 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4081 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4141 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4201 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4261 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4321 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4381 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4441 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4501 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4561 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4621 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4681 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4741 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4801 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4861 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4921 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     4981 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5041 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5101 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5161 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5221 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5281 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5341 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5401 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5461 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5521 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5581 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5641 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5701 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5761 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5821 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5881 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5941 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6001 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6061 catgcat
//
