LOCUS       40924_26974        4320 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pKR147, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Rossger K, Charpin-El Hamri G, Fussenegger M.
  TITLE     Reward-based hypertension control by a synthetic brain-dopamine 
            interface
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 110 (45), 18150-18155 (2013)
  PUBMED    24127594
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Roessger K, Fussenegger M, Charpin-El-Hamri G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel, 
            BS 4058, Switzerland
REFERENCE   3  (bases 1 to 4320)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4320)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2013"; volume: "110"; issue: "45"; pages:
            "18150-18155"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel, BS 4058, 
            Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             23..976
                     /codon_start=1
                     /product="ANP-IgG"
                     /label=ANP-IgG
                     /note="extendin-4-furin cleavage site-ANP-linker-mIgG-Fc"
                     /protein_id="AHA35650.1"
                     /translation="MKIILWLCVFGLFLATLFPISWQMPVESGLSSEDSASSESFAKRI
                     KRSLRRSSCFGGRIDRIGAQSGLGCNSFRYRRGSGGSGGSGGSGGSGGRSGCKPCICTV
                     PEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPRE
                     EQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIP
                     PPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLN
                     VQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK"
     polyA_signal    complement(1016..1137)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1556..2144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2318..3175)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3176..3280)
                     /label=AmpR promoter
     rep_origin      3307..3762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    3893..3941
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3955..4046
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     regulatory      4080..4319
                     /label=CRE promoter
                     /note="CRE promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 gaagctttcg cgagccgcca ccatgaagat catcctgtgg ctgtgtgtgt tcggcctgtt
       61 cctggccacc ctgttcccca tcagctggca gatgcccgtg gagtccggcc tgtcctccga
      121 ggactccgcc agctccgaga gcttcgccaa gcgcatcaag cgcagcctac gaagatccag
      181 ctgcttcggg ggtaggattg acaggattgg agcccagagt ggactaggct gcaacagctt
      241 ccggtaccga agaggatcag gaggatcagg aggatcagga ggatcaggag gatcaggagg
      301 aagatctggt tgtaagcctt gcatatgtac agtcccagaa gtatcatctg tcttcatctt
      361 ccccccaaag cccaaggatg tgctcaccat tactctgact cctaaggtca cgtgtgttgt
      421 ggtagacatc agcaaggatg atcccgaggt ccagttcagc tggtttgtag atgatgtgga
      481 ggtgcacaca gctcagacgc aaccccggga ggagcagttc aacagcactt tccgctcagt
      541 cagtgaactt cccatcatgc accaggactg gctcaatggc aaggagttca aatgcagggt
      601 caacagtgca gctttccctg cccccatcga gaaaaccatc tccaaaacca aaggcagacc
      661 gaaggctcca caggtgtaca ccattccacc tcccaaggag cagatggcca aggataaagt
      721 cagtctgacc tgcatgataa cagacttctt ccctgaagac attactgtgg agtggcagtg
      781 gaatgggcag ccagcggaga actacaagaa cactcagccc atcatggaca cagatggctc
      841 ttacttcgtc tacagcaagc tcaatgtgca gaagagcaac tgggaggcag gaaatacttt
      901 cacctgctct gtgttacatg agggcctgca caaccaccat actgagaaga gcctctccca
      961 ctctcctggt aaatgatcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     1021 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1081 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1141 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1201 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
     1261 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     1321 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     1381 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1441 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1501 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1561 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1621 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1681 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1741 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1801 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1861 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1921 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1981 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2041 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     2941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3181 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3301 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3361 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3421 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3481 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3541 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3601 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3661 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3721 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccattcg ccattcaggc
     3781 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc
     3841 taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc gcaataaaat
     3901 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
     3961 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
     4021 aagtgcaggt gccagaacat ttctctatcg ataggtaccg agctcttacg cgtgctagcg
     4081 caccagacag tgacgtcagc tgccagatcc catggccgtc atactgtgac gtctttcaga
     4141 caccccattg acgtcaatgg gagaacagat ctgccgcccc gactgcatct gcgtgttcga
     4201 attcgccaat gacaagacgc tgggcggggt ttgtgtcatc atagaactaa agacatgcaa
     4261 atatatttct tccggggaca ccgccagcaa acgcgagcaa cgggccacgg ggatgaagca
//
