LOCUS       V005197                14508 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005197
VERSION     V005197
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14508)
  AUTHORS   Piatkov K, Graciet E, Varshavsky A.
  TITLE     Ubiquitin Reference Technique and Its Use in Ubiquitin-Lacking
            Prokaryotes
  JOURNAL   PLoS ONE 8 (6), E67952 (2013)
   PUBMED   23825692
REFERENCE   2  (bases 1 to 14508)
  AUTHORS   Piatkov K, Graciet E, Varshavsky A.
  TITLE     Broad host range UPR vectors for Gram-negative Bacteria
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 14508)
  AUTHORS   Piatkov K, Graciet E, Varshavsky A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2012) Biology, California Institute of Technology,
            1200 E. California Blvd., 147-75, Pasadena, CA 91125, USA
REFERENCE   4  (bases 1 to 14508)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 14508)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2013"; volume: "8"; issue: "6"; pages: "E67952"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (14-JUN-2012) Biology, California Institute of Technology, 1200 E.
            California Blvd., 147-75, Pasadena, CA 91125, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..33)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             complement(46..69)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             complement(82..105)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    complement(115..129)
                     /label="cloning site"
                     /note="cloning site"
     CDS             complement(130..357)
                     /label="ubiquitin"
                     /note="Saccharomyces cerevisiae ubiquitin"
     CDS             complement(385..411)
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(418..978)
                     /label="DHFR"
                     /note="mouse dihydrofolate reductase"
     CDS             complement(991..1014)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             complement(1027..1050)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             complement(1063..1086)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     protein_bind    1571..1688
                     /label="UAS"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     CDS             2387..4813
                     /gene="UBP1"
                     /label="Ubiquitin carboxyl-terminal hydrolase 1"
                     /note="Ubiquitin carboxyl-terminal hydrolase 1 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P25037"
     promoter        5096..5124
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             5336..6139
                     /codon_start=1
                     /product="Sm resistance protein A"
                     /label="Sm resistance protein A"
                     /protein_id="AFQ55265.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     CDS             6139..6975
                     /codon_start=1
                     /product="Sm resistance protein B"
                     /label="Sm resistance protein B"
                     /protein_id="AFQ55266.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     rep_origin      complement(7619..8013)
                     /direction=LEFT
                     /label="RSF1010 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     RSF1010; requires the RSF1010 RepA/B/C proteins for
                     replication (Scholz et al., 1989)"
     CDS             complement(8040..8324)
                     /codon_start=1
                     /product="mobilization protein C"
                     /label="mobilization protein C"
                     /protein_id="AFQ55267.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     oriT            8355..8442
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
     CDS             9271..9684
                     /codon_start=1
                     /product="mobilization protein B"
                     /label="mobilization protein B"
                     /protein_id="AFQ55269.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     CDS             9681..10649
                     /label="RSF1010 RepB"
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             10713..10925
                     /codon_start=1
                     /product="unknown"
                     /label="unknown"
                     /note="E"
                     /protein_id="AFQ55271.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             10927..11133
                     /codon_start=1
                     /product="repressor protein F"
                     /label="repressor protein F"
                     /protein_id="AFQ55272.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             11163..11999
                     /label="RSF1010 RepA"
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             11989..12837
                     /label="RSF1010 RepC"
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             complement(13365..14156)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gctagattac ttgtcatcgt cgtccttgta gtcagagccg ctccccttat cgtcgtcatc
       61 tttgtaatcg gaaccagagc ctttgtcgtc atcatcctta tagtcggatc cggagagacc
      121 taggtctcta ccacctctta gccttagcac aagatgtaag gtggactcct tctgaatgtt
      181 gtaatcagac agcgttctac cgtcttctag ctgcctaccg gcaaagatca atctttgttg
      241 atctggaggg ataccttcct tgtcttgaat tttcgactta acgttgtcga tggtatcgga
      301 agattcaact tccaatgtta tggttttacc ggtcaaagtc ttgacgaaaa tctgcatggt
      361 cgacccacta cccgcaagga aagcggcgta atctgggaca tcgtatgggt aggtaccgtc
      421 tttcttctcg tagacttcaa acttatactt gatgcctttt tcctcctgga cctcagagag
      481 gacgcctggg tattctggga gaagtttata tttccccaaa tcaatttctg ggaaaaacgt
      541 gtcactttca aattcctgca tgatccttgt cacaaagagt ctaaggtggc ctggttgatt
      601 catggcttcc tggtaaacag aactgcctcc gactatccaa accatgtcta ctttacttgc
      661 caattccggt tgttcaataa gtcttaaggc atcatccaaa cttttggcaa gaaaatgagc
      721 tcctcgtggt ggttctttga gttctctact gagaactata ttaattctgt cctttaaagg
      781 tcgattcttc tcaggaatgg agaaccaggt tttcctaccc ataatcacca gattctgttt
      841 accttccact gaagaggttg tggtcattct ttggaagtac ttgaactcgt tcctgagcgg
      901 aggccagggt aggtcaccgt tcttgccaat ccccatattt tgggacacgg cgacgatgca
      961 gttcaatggt cgaaccatat gggatccaga cttgtcatcg tcgtccttgt agtcagagcc
     1021 gctcccctta tcgtcgtcat ctttgtaatc ggaaccagag cctttgtcgt catcatcctt
     1081 atagtcaccc atggtatatc tccttcttaa agtaaagtgg gagggagagt tcgagagttc
     1141 ttttcgtatt gtagtagata gaagatatac ttatccttat ccgtacagtt cttgctcttt
     1201 ctgaagtagc actttcggaa gaagaaagat aagcaggcga attgatccgg taatttagtg
     1261 tgtgtatttg tgtttgcgtg tctatagaag tatagtaatt tatgctacaa aggacctaat
     1321 gtataaggaa agaatattta gagaaaagaa gaaaacaaga gttttatata catacagagc
     1381 acatgcatgc tatatgatca tgtgtcgtcg cacacatata tatatgcctg tatgtgtcag
     1441 cactaaagtt gcctggccat ccacgctata tacacgcctg gcggatctgc tcgaggtcga
     1501 ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta
     1561 tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc
     1621 tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca
     1681 ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag aggaaaattg
     1741 gcagtaacct ggccccacaa ccttcaatga acgaatcaat taccaccata ggatgataat
     1801 gcgattagtt ttttagccta tttctggggt attatcagcg agcgatgatt tggatcgggc
     1861 ggccgcagat ctgtaagccg taatgttttt cccataaacg tcactgaatg tccatatatc
     1921 atactaccag taagcgcctg ttggaaacct tcctaccttc atacgtgtat tctgctatat
     1981 cccttctttt cctctttacg tacagaatga catctatgta ggcaagtttc tttttccatc
     2041 attggaaaac gaaaatacat ttttcactag caccacaaaa ttttcaagat ggccaggcta
     2101 gttaaatcta ttatacaata attattggtt atatatttaa aaaacagaaa cattttttgc
     2161 tttaaaagca cgccatagta ttgcttttaa tattgtgatc tgcgtccttt tttttctcag
     2221 gaaaaaaaaa ttttatacac attcaaagaa tagaagcgat tgtcaaaatt cgcttctcct
     2281 ttcttttcca ttataacgtc tgatcatttt acgtcttcag tgccctccct tgttcgaaac
     2341 tagatacttt cgaacacttc tcccctttta atctacaaaa ttttgtatgg atttgtttat
     2401 tgaaagcaag ataaacagtt tattacaatt tttatttggt tcccgacagg attttttgag
     2461 aaattttaaa acttggagta acaacaataa caatctatcg atttatttat taatttttgg
     2521 catagtagta tttttttata aaaaaccaga ccatctaaac tacattgttg agagcgttag
     2581 tgaaatgaca acaaacttca gaaataataa tagccttagc cgttggttgc ccagaagtaa
     2641 gtttacccac ttagacgaag agatcttgaa aagaggtggt ttcattgctg gtttagttaa
     2701 tgatggtaac acttgtttta tgaactctgt tttgcaatca ttggcatcat ccagagaatt
     2761 aatggagttc ttggacaata atgtcataag gacctatgag gagatagaac aaaatgaaca
     2821 caatgaagaa ggaaacgggc aagaatctgc tcaagatgaa gccactcata agaaaaacac
     2881 tcgtaagggt ggcaaagttt atggtaagca taagaagaaa ttgaatagga agtcaagttc
     2941 gaaagaagac gaagaaaaga gccaggagcc agatatcact ttcagtgtcg ccttaaggga
     3001 tctactttct gccttaaatg cgaagtatta tcgggataaa ccctatttca aaaccaatag
     3061 tttattgaaa gcaatgtcca aatctccaag aaaaaatatt cttcttggct acgaccaaga
     3121 ggacgcgcaa gaattcttcc agaacatact agccgagttg gaaagtaacg ttaaatcatt
     3181 gaatactgaa aaactagata ccactccagt tgcgaaatca gaattacccg atgatgcttt
     3241 agtaggtcaa cttaaccttg gtgaagttgg cactgtttac attccaactg aacagattga
     3301 tcctaactct atactacatg acaagtccat tcaaaatttc acacctttca aactaatgac
     3361 tcctttagat ggtatcacgg cagaaagaat tggttgttta cagtgtggtg agaacggtgg
     3421 cataagatat tccgtatttt cgggattaag cttaaattta ccgaacgaga atattggttc
     3481 cactttaaaa ttatctcagt tattaagcga ctggagtaaa cctgaaatca tcgaaggcgt
     3541 agaatgtaac cgttgtgccc tcacagcagc gcactctcat ttatttggtc agttgaaaga
     3601 atttgaaaaa aaacctgagg gttcgatccc agaaaagtta attaacgctg taaaagatag
     3661 ggtccatcaa atcgaagaag ttcttgccaa accagttatt gacgatgaag attataagaa
     3721 gttgcataca gcaaatatgg tacgtaaatg ctctaaatct aagcagattt taatatcaag
     3781 acctccacca ttattatcca ttcatatcaa cagatccgta tttgatccaa gaacgtacat
     3841 gattagaaaa aataactcga aagtattgtt taagtcaagg ttgaatcttg ccccatggtg
     3901 ttgtgatatt aatgaaatca atttggatgc tcgtttgcca atgtcaaaaa aggaaaaagc
     3961 tgcgcaacaa gattcaagtg aagatgaaaa cattggcggt gaatactata cgaaattaca
     4021 tgaacgcttc gagcaggaat ttgaagacag cgaggaagaa aaagaatacg atgacgcaga
     4081 ggggaactat gcgtctcatt acaatcatac caaggatatc agtaactatg atcccctaaa
     4141 cggtgaagtc gatggcgtga catccgatga tgaagatgag tacattgaag aaaccgatgc
     4201 tttagggaat acaatcaaaa aaaggatcat agaacattct gatgttgaaa acgagaatgt
     4261 aaaagataat gaagaactgc aagaaatcga caatgtgagc cttgacgaac caaagatcaa
     4321 tgttgaagat caactagaaa catcatctga tgaggaagat gttataccag ctccacctat
     4381 caattatgct aggtcatttt ccacagttcc agccactcca ttgacatatt cattgcgctc
     4441 tgtcattgtt cactacggta cccataatta tggtcattac attgcattta gaaaatacag
     4501 gggttgttgg tggagaatat ctgatgagac tgtgtacgtt gtggacgaag ctgaagtcct
     4561 ttcaacaccc ggtgtattta tgttatttta cgaatatgac tttgatgaag aaactgggaa
     4621 gatgaaggat gatttggaag ctattcagag taataatgaa gaagatgatg aaaaagagca
     4681 ggagcaaaaa ggagtccagg agccaaagga aagccaagag caaggagaag gtgaagagca
     4741 agaggaaggt caagagcaga tgaagttcga gagaacagaa gaccatagag atatttctgg
     4801 taaagatgta aactaagtta taaatacgat atccgtaatt gtgtaaataa caataactat
     4861 aattaaattg aataattaaa agtctgtcga cctgcatatg atcttaaggc ctaggtctag
     4921 agtctttgtt ttgacgccat tagcgtacgt aacaatcctc gttaaaggac aaggacctga
     4981 gcggaagtgt atcgtacagt agacggagta tactagtata gtctatagtc cgtggaatta
     5041 ttatatttat ctccgacgat attctcatca gtgaaatcca ggggaattct catgtttgac
     5101 agcttatcat cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc
     5161 aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg
     5221 atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg gataactgca
     5281 cattcgggat atttctctat attcgcgctt catcagaaaa ctgaaggaac ctccattgaa
     5341 tcgaactaat attttttttg gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga
     5401 atctggtgat tttgtttttc gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc
     5461 ttcccgccgc ggtgagctcg ctggagagcg tgaccgcctc atttggctca aaggtcgagg
     5521 tgtggcttgc cccgaggtca tcaactggca ggaggaacag gagggtgcat gcttggtgat
     5581 aacggcaatt ccgggagtac cggcggctga tctgtctgga gcggatttgc tcaaagcgtg
     5641 gccgtcaatg gggcagcaac ttggcgctgt tcacagccta tcggttgatc aatgtccgtt
     5701 tgagcgcagg ctgtcgcgaa tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt
     5761 caatcccgac ttcttaccgg acgaggacaa gagtacgccg ctgcacgatc ttttggctcg
     5821 tgtcgaacga gagctaccgg tgcggctcga ccaagagcgc accgatatgg ttgtttgcca
     5881 tggtgatccc tgcatgccga acttcatggt ggaccctaaa actcttcaat gcacgggtct
     5941 gatcgacctt gggcggctcg gaacagcaga tcgctatgcc gatttggcac tcatgattgc
     6001 taacgccgaa gagaactggg cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt
     6061 caatgtattg gggatcgaag cccccgaccg cgaacgcctt gccttctatc tgcgattgga
     6121 ccctctgact tggggttgat gttcatgccg cctgtttttc ctgctcattg gcacgtttcg
     6181 caacctgttc tcattgcgga caccttttcc agcctcgttt ggaaagtttc attgccagac
     6241 gggactcctg caatcgtcaa gggattgaaa cctatagaag acattgctga tgaactgcgc
     6301 ggggccgact atctggtatg gcgcaatggg aggggagcag tccggttgct cggtcgtgag
     6361 aacaatctga tgttgctcga atatgccggg gagcgaatgc tctctcacat cgttgccgag
     6421 cacggcgact accaggcgac cgaaattgca gcggaactaa tggcgaagct gtatgccgca
     6481 tctgaggaac ccctgccttc tgcccttctc ccgatccggg atcgctttgc agctttgttt
     6541 cagcgggcgc gcgatgatca aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt
     6601 atagccgatc aaatgatgag caatgcctcg gaactgcgtg ggctacatgg cgatctgcat
     6661 catgaaaaca tcatgttctc cagtcgcggc tggctggtga tagatcccgt cggtctggtc
     6721 ggtgaagtgg gctttggcgc cgccaatatg ttctacgatc cggctgacag agacgacctt
     6781 tgtctcgatc ctagacgcat tgcacagatg gcggacgcat tctctcgtgc gctggacgtc
     6841 gatccgcgtc gcctgctcga ccaggcgtac gcttatgggt gcctttccgc agcttggaac
     6901 gcggatggag aagaggagca acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga
     6961 cagacgtcat actagatatc aagcgacttc tcctatcccc tgggaacaca tcaatctcac
     7021 cggagaatat cgctggccaa agccttagcg taggattccg ccccttcccg caaacgaccc
     7081 caaacaggaa acgcagctga aacgggaagc tcaacaccca ctgacgcatg ggttgttcag
     7141 gcagtacttc atcaaccagc aaggcggcac tttcggccat ccgccgcgcc ccacagctcg
     7201 ggcagaaacc gcgacgctta cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc
     7261 agcgaacccg tagaaagcca tgctccagcc gcccgcattg gagaaattct tcaaattccc
     7321 gttgcacata gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt
     7381 aatactcgtc aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac
     7441 cagtatcccg atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg
     7501 caatactgtg tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa
     7561 atgcctgccg ttgctagaca ttgccagcca gtgcccgtca ctcccgtact aactgtcacg
     7621 aacccctgca ataactgtca cgcccccctg caataactgt cacgaacccc tgcaataact
     7681 gtcacgcccc caaacctgca aacccagcag gggcgggggc tggcggggtg ttggaaaaat
     7741 ccatccatga ttatctaaga ataatccact aggcgcggtt atcagcgccc ttgtggggcg
     7801 ctgctgccct tgcccaatat gcccggccag aggccggata gctggtctat tcgctgcgct
     7861 aggctacaca ccgccccacc gctgcgcggc agggggaaag gcgggcaaag cccgctaaac
     7921 cccacaccaa accccgcaga aatacgctgg agcgctttta gccgctttag cggcctttcc
     7981 ccctacccga agggtggggg cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt
     8041 cagcccggct catccttctg gcgtggcggc agaccgaaca aggcgcggtc gtggtcgcgt
     8101 tcaaggtacg catccattgc cgccatgagc cgatcctccg gccactcgct gctgttcacc
     8161 ttggccaaaa tcatggcccc caccagcacc ttgcgccttg tttcgttctt gcgctcttgc
     8221 tgctgttccc ttgcccgcac ccgctgaatt tcggcattga ttcgcgctcg ttgttcttcg
     8281 agcttggcca gccgatccgc cgccttgttg ctccccttaa ccatcttgac accccattgt
     8341 taatgtgctg tctcgtaggc tatcatggag gcacagcggc ggcaatcccg accctacttt
     8401 gtaggggagg gcgcacttac cggtttctct tcgagaaact ggcctaacgg ccacccttcg
     8461 ggcggtgcgc tctccgaggg ccattgcatg gagccgaaaa gcaaaagcaa cagcgaggca
     8521 gcatggcgat ttatcacctt acggcgaaaa ccggcagcag gtcgggcggc caatcggcca
     8581 gggccaaggc cgactacatc cagcgcgaag gcaagtatgc ccgcgacatg gatgaagtct
     8641 tgcacgccga atccgggcac atgccggagt tcgtcgagcg gcccgccgac tactgggatg
     8701 ctgccgacct gtatgaacgc gccaatgggc ggctgttcaa ggaggtcgaa tttgccctgc
     8761 cggtcgagct gaccctcgac cagcagaagg cgctggcgtc cgagttcgcc cagcacctga
     8821 ccggtgccga gcgcctgccg tatacgctgg ccatccatgc cggtggcggc gagaacccgc
     8881 actgccacct gatgatctcc gagcggatca atgacggcat cgagcggccc gccgctcagt
     8941 ggttcaagcg gtacaacggc aagaccccgg agaagggcgg ggcacagaag accgaagcgc
     9001 tcaagcccaa ggcatggctt gagcagaccc gcgaggcatg ggccgaccat gccaaccggg
     9061 cattagagcg ggctggccac gacgcccgca ttgaccacag aacacttgag gcgcagggca
     9121 tcgagcgcct gcccggtgtt cacctggggc cgaacgtggt ggagatggaa ggccggggca
     9181 tccgcaccga ccgggcagac gtggccctga acatcgacac cgccaacgcc cagatcatcg
     9241 acttacagga ataccgggag gcaatagacc atgaacgcaa tcgacagagt gaagaaatcc
     9301 agaggcatca acgagttagc ggagcagatc gaaccgctgg cccagagcat ggcgacactg
     9361 gccgacgaag cccggcaggt catgagccag acccagcagg ccagcgaggc gcaggcggcg
     9421 gagtggctga aagcccagcg ccagacaggg gcggcatggg tggagctggc caaagagttg
     9481 cgggaggtag ccgccgaggt gagcagcgcc gcgcagagcg cccggagcgc gtcgcggggg
     9541 tggcactgga agctatggct aaccgtgatg ctggcttcca tgatgcctac ggtggtgctg
     9601 ctgatcgcat cgttgctctt gctcgacctg acgccactga caaccgagga cggctcgatc
     9661 tggctgcgct tggtggcccg atgaagaacg acaggacttt gcaggccata ggccgacagc
     9721 tcaaggccat gggctgtgag cgcttcgata tcggcgtcag ggacgccacc accggccaga
     9781 tgatgaaccg ggaatggtca gccgccgaag tgctccagaa cacgccatgg ctcaagcgga
     9841 tgaatgccca gggcaatgac gtgtatatca ggcccgccga gcaggagcgg catggtctgg
     9901 tgctggtgga cgacctcagc gagtttgacc tggatgacat gaaagccgag ggccgggagc
     9961 ctgccctggt agtggaaacc agcccgaaga actatcaggc atgggtcaag gtggccgacg
    10021 ccgcaggcgg tgaacttcgg gggcagattg cccggacgct ggccagcgag tacgacgccg
    10081 acccggccag cgccgacagc cgccactatg gccgcttggc gggcttcacc aaccgcaagg
    10141 acaagcacac cacccgcgcc ggttatcagc cgtgggtgct gctgcgtgaa tccaagggca
    10201 agaccgccac cgctggcccg gcgctggtgc agcaggctgg ccagcagatc gagcaggccc
    10261 agcggcagca ggagaaggcc cgcaggctgg ccagcctcga actgcccgag cggcagctta
    10321 gccgccaccg gcgcacggcg ctggacgagt accgcagcga gatggccggg ctggtcaagc
    10381 gcttcggtga tgacctcagc aagtgcgact ttatcgccgc gcagaagctg gccagccggg
    10441 gccgcagtgc cgaggaaatc ggcaaggcca tggccgaggc cagcccagcg ctggcagagc
    10501 gcaagcccgg ccacgaagcg gattacatcg agcgcaccgt cagcaaggtc atgggtctgc
    10561 ccagcgtcca gcttgcgcgg gccgagctgg cacgggcacc ggcaccccgc cagcgaggca
    10621 tggacagggg cgggccagat ttcagcatgt agtgcttgcg ttggtactca cgcctgttat
    10681 actatgagta ctcacgcaca gaagggggtt ttatggaata cgaaaaaagc gcttcagggt
    10741 cggtctacct gatcaaaagt gacaagggct attggttgcc cggtggcttt ggttatacgt
    10801 caaacaaggc cgaggctggc cgcttttcag tcgctgatat ggccagcctt aaccttgacg
    10861 gctgcacctt gtccttgttc cgcgaagaca agcctttcgg ccccggcaag tttctcggtg
    10921 actgatatga aagaccaaaa ggacaagcag accggcgacc tgctggccag ccctgacgct
    10981 gtacgccaag cgcgatatgc cgagcgcatg aaggccaaag ggatgcgtca gcgcaagttc
    11041 tggctgaccg acgacgaata cgaggcgctg cgcgagtgcc tggaagaact cagagcggcg
    11101 cagggcgggg gtagtgaccc cgccagcgcc taaccaccaa ctgcctgcaa aggaggcaat
    11161 caatggctac ccataagcct atcaatattc tggaggcgtt cgcagcagcg ccgccaccgc
    11221 tggactacgt tttgcccaac atggtggccg gtacggtcgg ggcgctggtg tcgcccggtg
    11281 gtgccggtaa atccatgctg gccctgcaac tggccgcaca gattgcaggc gggccggatc
    11341 tgctggaggt gggcgaactg cccaccggcc cggtgatcta cctgcccgcc gaagacccgc
    11401 ccaccgccat tcatcaccgc ctgcacgccc ttggggcgca cctcagcgcc gaggaacggc
    11461 aagccgtggc tgacggcctg ctgatccagc cgctgatcgg cagcctgccc aacatcatgg
    11521 ccccggagtg gttcgacggc ctcaagcgcg ccgccgaggg ccgccgcctg atggtgctgg
    11581 acacgctgcg ccggttccac atcgaggaag aaaacgccag cggccccatg gcccaggtca
    11641 tcggtcgcat ggaggccatc gccgccgata ccgggtgctc tatcgtgttc ctgcaccatg
    11701 ccagcaaggg cgcggccatg atgggcgcag gcgaccagca gcaggccagc cggggcagct
    11761 cggtactggt cgataacatc cgctggcagt cctacctgtc gagcatgacc agcgccgagg
    11821 ccgaggaatg gggtgtggac gacgaccagc gccggttctt cgtccgcttc ggtgtgagca
    11881 aggccaacta tggcgcaccg ttcgctgatc ggtggttcag gcggcatgac ggcggggtgc
    11941 tcaagcccgc cgtgctggag aggcagcgca agagcaaggg ggtgccccgt ggtgaagcct
    12001 aagaacaagc acagcctcag ccacgtccgg cacgacccgg cgcactgtct ggcccccggc
    12061 ctgttccgtg ccctcaagcg gggcgagcgc aagcgcagca agctggacgt gacgtatgac
    12121 tacggcgacg gcaagcggat cgagttcagc ggcccggagc cgctgggcgc tgatgatctg
    12181 cgcatcctgc aagggctggt ggccatggct gggcctaatg gcctagtgct tggcccggaa
    12241 cccaagaccg aaggcggacg gcagctccgg ctgttcctgg aacccaagtg ggaggccgtc
    12301 accgctgaat gccatgtggt caaaggtagc tatcgggcgc tggcaaagga aatcggggca
    12361 gaggtcgata gtggtggggc gctcaagcac atacaggact gcatcgagcg cctttggaag
    12421 gtatccatca tcgcccagaa tggccgcaag cggcaggggt ttcggctgct gtcggagtac
    12481 gccagcgacg aggcggacgg gcgcctgtac gtggccctga accccttgat cgcgcaggcc
    12541 gtcatgggtg gcggccagca tgtgcgcatc agcatggacg aggtgcgggc gctggacagc
    12601 gaaaccgccc gcctgctgca ccagcggctg tgtggctgga tcgaccccgg caaaaccggc
    12661 aaggcttcca tagatacctt gtgcggctat gtctggccgt cagaggccag tggttcgacc
    12721 atgcgcaagc gccgccagcg ggtgcgcgag gcgttgccgg agctggtcgc gctgggctgg
    12781 acggtaaccg agttcgcggc gggcaagtac gacatcaccc ggcccaaggc ggcaggctga
    12841 ccccccccac tctattgtaa acaagacatt tttatctttt atattcaatg gcttattttc
    12901 ctgctaattg gtaataccat gaaaaatacc atgctcagaa aaggcttaac aatattttga
    12961 aaaattgcct actgagcgct gccgcacagc tccataggcc gctttcctgg ctttgcttcc
    13021 agatgtatgc tcttctgctc ctgcagttcg ggggcatgga tgcgcggata gccgctgctg
    13081 gtttcctgga tgccgacgga tttgcactgc cggtagaact ccgcgaggtc gtccagcctc
    13141 aggcagcagc tgaaccaact cgcgagggga tcgagcccgg ggtgggcgaa gaactccagc
    13201 atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc
    13261 aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct
    13321 tggtcggtca tttcgcctcg aggcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
    13381 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
    13441 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
    13501 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
    13561 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
    13621 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
    13681 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
    13741 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
    13801 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
    13861 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
    13921 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
    13981 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
    14041 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
    14101 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
    14161 aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc
    14221 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
    14281 gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta
    14341 agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc
    14401 cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc
    14461 cgcttccttt agcagccctt gcgccctgag tgcttgcggc agcgtgaa
//
