LOCUS       Exported                6689 bp DNA     circular SYN 19-JUL-2025
DEFINITION  Exported.
ACCESSION   V005198
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Kostner D, Peters B, Mientus M, Liebl W, Ehrenreich A.
  TITLE     Importance of codB for new codA-based markerless gene deletion in 
            Gluconobacter strains
  JOURNAL   Appl. Microbiol. Biotechnol. 97 (18), 8341-8349 (2013)
  PUBMED    23955475
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Kostner D, Liebl W, Ehrenreich A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2012) Department of Microbiology, TU Munich, Emil 
            Ramannstr. 4, Freising, Bayern 85354, Germany
REFERENCE   3  (bases 1 to 6689)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6689)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6689)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2013"; volume: "97"; issue: "18"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2012) Department of Microbiology, TU Munich, Emil Ramannstr.
            4, Freising, Bayern 85354, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             401..1192
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1495..2751
                     /gene="codB"
                     /label=Cytosine permease
                     /note="Cytosine permease from Escherichia coli (strain
                     K12). Accession#: P0AA82"
     CDS             2744..4024
                     /label=codA
                     /note="E. coli cytosine deaminase"
     oriT            complement(4432..4541)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        complement(4776..4866)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(5060..5150)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      5510..6098
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6386..6407
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6422..6452
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6460..6476
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6484..6500
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6509..6565
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6569..6585)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccttcccaac agttgcgcag cctgaatggc gaatggcgat aagctagctt cacgctgccg
       61 caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag ccagtccgca
      121 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
      181 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
      241 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
      301 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
      361 atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
      421 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
      481 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
      541 ttttgtcaag accgacctgt ccggtgccct gaatgaactc caagacgagg cagcgcggct
      601 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      661 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      721 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      781 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      841 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      901 agccgaactg ttcgccaggc tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac
      961 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     1021 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     1081 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     1141 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     1201 actctggggt tcggatccgg gcttatcgac tgcacggtgc accaatgctt ctggcgtcag
     1261 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc
     1321 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
     1381 caaatattca tgacagctta agatgcgtga caaaatacgg ccgcgctttc atacatcgtc
     1441 agaagacgcc tttcagattt tccggagaac caaacagcaa aaagggggaa tttcgtgtcg
     1501 caagataaca actttagcca ggggccagtc ccgcagtcgg cgcggaaagg ggtattggca
     1561 ttgacgttcg tcatgctggg attaaccttc ttttccgcca gtatgtggac cggcggcact
     1621 ctcggaaccg gtcttagcta tcatgatttc ttcctcgcag ttctcatcgg taatcttctc
     1681 ctcggtattt acacttcatt tctcggttac attggcgcaa aaaccggcct gaccactcat
     1741 cttcttgctc gcttctcgtt tggtgttaaa ggctcatggc tgccttcact gctactgggc
     1801 ggaactcagg ttggctggtt tggcgtcggt gtggcgatgt ttgccattcc ggtgggtaag
     1861 gcaaccgggc tggatattaa tttgctgatt gccgtttccg gtttactgat gaccgtcacc
     1921 gtcttttttg gcatttcggc gctgacggtt ctttcggtga ttgcggttcc ggctatcgcc
     1981 tgcctgggcg gttattccgt gtggctggct gttaacggca tgggcggcct ggacgcatta
     2041 aaagcggtcg ttcccgcaca accgttagat ttcaatgtcg cgctggcgct ggttgtgggg
     2101 tcatttatca gtgcgggtac gctcaccgct gactttgtcc ggtttggtcg caatgccaaa
     2161 ctggcggtgc tggtggcgat ggtggccttt ttcctcggca actcgttgat gtttattttc
     2221 ggtgcagcgg gcgctgcggc actgggcatg gcggatatct ctgatgtgat gattgctcag
     2281 ggcctgctgc tgcctgcgat tgtggtgctg gggctgaata tctggaccac caacgataac
     2341 gcactctatg cgtcgggttt aggtttcgcc aacattaccg ggatgtcgag caaaaccctt
     2401 tcggtaatca acggtattat cggtacggtc tgcgcattat ggctgtataa caattttgtc
     2461 ggctggttga ccttcctttc ggcagctatt cctccagtgg gtggcgtgat catcgccgac
     2521 tatctgatga accgtcgccg ctatgagcac tttgcgacca cgcgtatgat gagtgtcaat
     2581 tgggtggcga ttctggcggt cgccttgggg attgctgcag gccactggtt accgggaatt
     2641 gttccggtca acgcggtatt aggtggcgcg ctgagctatc tgatccttaa cccgattttg
     2701 aatcgtaaaa cgacagcagc aatgacgcat gtggaggcta acagtgtcga ataacgcttt
     2761 acaaacaatt attaacgccc ggttaccagg cgaagagggg ctgtggcaga ttcatctgca
     2821 ggacggaaaa atcagcgcca ttgatgcgca atccggcgtg atgcccataa ctgaaaacag
     2881 cctggatgcc gaacaaggtt tagttatacc gccgtttgtg gagccacata ttcacctgga
     2941 caccacgcaa accgccggac aaccgaactg gaatcagtcc ggcacgctgt ttgaaggcat
     3001 tgaacgctgg gccgagcgca aagcgttatt aacccatgac gatgtgaaac aacgcgcatg
     3061 gcaaacgctg aaatggcaga ttgccaacgg cattcagcat gtgcgtaccc atgtcgatgt
     3121 ttcggatgca acgctaactg cgctgaaagc aatgctggaa gtgaagcagg aagtcgcgcc
     3181 gtggattgat ctgcaaatcg tcgccttccc tcaggaaggg attttgtcgt atcccaacgg
     3241 tgaagcgttg ctggaagagg cgttacgctt aggggcagat gtagtggggg cgattccgca
     3301 ttttgaattt acccgtgaat acggcgtgga gtcgctgcat aaaaccttcg ccctggcgca
     3361 aaaatacgac cgtctcatcg acgttcactg tgatgagatc gatgacgagc agtcgcgctt
     3421 tgtcgaaacc gttgctgccc tggcgcacca tgaaggcatg ggcgcgcgag tcaccgccag
     3481 ccacaccacg gcaatgcact cctataacgg ggcgtatacc tcacgcctgt tccgcttgct
     3541 gaaaatgtcc ggtattaact ttgtcgccaa cccgctggtc aatattcatc tgcaaggacg
     3601 tttcgatacg tatccaaaac gtcgcggcat cacgcgcgtt aaagagatgc tggagtccgg
     3661 cattaacgtc tgctttggtc acgatgatgt cttcgatccg tggtatccgc tgggaacggc
     3721 gaatatgctg caagtgctgc atatggggct gcatgtttgc cagttgatgg gctacgggca
     3781 gattaacgat ggcctgaatt taatcaccca ccacagcgca aggacgttga atttgcagga
     3841 ttacggcatt gccgccggaa acagcgccaa cctgattatc ctgccggctg aaaatgggtt
     3901 tgatgcgctg cgccgtcagg ttccggtacg ttattcggta cgtggcggca aggtgattgc
     3961 cagcacacaa ccggcacaaa ccaccgtata tctggagcag ccagaagcca tcgattacaa
     4021 acgttgaacg actgggttac agcgagctta gtttatgccg gatctctcct ggcgcaccat
     4081 cgtcggctac agcctcggga attgctaccg cgaaatgacc gaccaagcga cgcccaacct
     4141 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     4201 tttccgggac gccctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct
     4261 ggccgacggc cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa
     4321 tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg
     4381 gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc
     4441 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
     4501 aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac
     4561 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg
     4621 gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgct
     4681 gcttccctgc tgttttgtgg aatatctacc gactggaaac aggcaaatgc aggaaattac
     4741 tgaactgagg ggacaggcga gagacgatgc caaagagctc ctgaaaatct cgataactca
     4801 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     4861 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
     4921 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt
     4981 gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc
     5041 agtggcttct gtttctatca gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     5101 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     5161 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     5221 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     5281 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     5341 atcagggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5401 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5461 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5521 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5581 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5641 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5701 tagcaccgcc tacatacctc gctctgctaa tcctgttacg agtggctgct gccagtggcg
     5761 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5821 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5881 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5941 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6001 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6061 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6121 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6181 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6241 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6301 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6361 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6421 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6481 acacaggaaa cagctatgac atgattacga attcgagctc ggtacccggg gatcctctag
     6541 agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
     6601 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6661 gtaatagcga agaggcccgc accgatcgc
//
