LOCUS       V016006                 4305 bp    DNA     circular SYN 01-SEP-2021
DEFINITION  Exported.
ACCESSION   V016006
VERSION     V016006
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Hend A, Yuka O, Tomoya K, Kouta S, Izumi N, Tohru S.
  TITLE     Bifidobacterium-E. coli shuttle vector series, pKO403, for metabolic
            engineering
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Hend A, Yuka O, Tomoya K, Kouta S, Izumi N, Tohru S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2021) Contact:Altaib Hend Gifu University, Applied
            Biological Science; 1-1Yanagido, Gifu, Gifu 501-1193, Japan
REFERENCE   3  (bases 1 to 4305)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-AUG-2021) Contact:Altaib Hend Gifu University, Applied
            Biological Science; 1-1Yanagido, Gifu, Gifu 501-1193, Japan"
FEATURES             Location/Qualifiers
     source          1..4305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..11
                     /note="SapI site; three base pairs of tag sequence is
                     included"
     primer_bind     334..350
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(354..410)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(420..436)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(444..460)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(468..498)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(513..534)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    638..648
                     /note="SapI site; three base pairs of tag sequence is
                     included"
     misc_feature    662..2286
                     /label="Ori-Ts"
                     /note="Ori-Ts"
     gene            1288..1978
                     /gene="repB"
                     /label="repB"
     misc_feature    1288..1978
                     /gene="repB"
     misc_feature    2301..2534
                     /label="hup terminator"
                     /note="hup terminator"
     rep_origin      complement(2617..3205)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    3363..3552
                     /label="hup promoter"
                     /note="hup promoter"
     CDS             3553..4200
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    4204..4263
                     /label="putative terminator"
                     /note="putative terminator"
ORIGIN
        1 acatgaagag cgtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
       61 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
      121 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
      181 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
      241 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      301 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg
      361 catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc gtaatcatgg
      421 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      481 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      541 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      601 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttcgtaggg ccgccctcag
      661 ggccaccgtc gccaaggctc ttgaacacaa cgtcaggacc agcgatggca tgaactggaa
      721 cctccaggac ctcgtctacg tggcgctgag cgaggaatgg cgcaaaaggg acggcgagat
      781 cagcgaccca tgggccaacg acgaggcgga cggataccag ccgccctcat acgagccggt
      841 caaccccgaa cgcaggactc cccagacgcc ctccgatggc ctgatctgac gtccgaaaaa
      901 aggcgctgtg cgcccttttt aaatctttta taaatctttt tacattcttt tagcccctcc
      961 gcagccttac tctcccaacg ggtttcagcc gaaacctaca ccaaaagggg agcgaaccta
     1021 caccaaaagg ggagcgaacc tacaccaaaa ggggagcgaa cctacaccaa aaggggagct
     1081 atatacacct tttgttattt aaggtgcaag ttgtgctatg ctgaggccat gtccaatgag
     1141 atcgtgaagt tcagcaacca gttcaacaac gtcgcgctga agaagttcga cgccgtgcac
     1201 ctggacgtgc tcatggcgat cgcctcaagg gtgagggaga agggcacggc cacggtggag
     1261 ttctcgttcg aggagctgcg cggcctcatg cgattgagga agaacctgac caacaagcag
     1321 ctggccgaca agatcgtgca gacgaacgcg cgcctgctgg cgctgaacta catgttcgag
     1381 gattcgggca agatcatcca gttcgcgctg ttcacgaagt tcgtcaccga cccgcaggag
     1441 gcgactctcg cggttggggt caacgaggag ttcgcgttcc tgctcaacga cctgaccagc
     1501 cagttcacgc gcttcgagct ggccgagttc gccgacctca agatcaagta cgccaaggag
     1561 ttctaccgca gggccaagca gtaccgcagc tccggaatct ggaagatcgg ccgcgacgag
     1621 ttctgccgac tgcttggcgt tccaccgtcg gcaataaccc agacacgata tctgaatcag
     1681 aaggttcttc agccaattca ggaggagtgt gggcctctcc ttggcctgaa gatcgagcgc
     1741 cagtacgtga aacgcaggct gtcgggcttc gtgttcacat tcgcccgcga gacccctccg
     1801 gtgatcgacg ccaggcccgt ggaggcgagg aagacggacg gcgacggcaa gggccattgg
     1861 acgagcgttg ccggatacgg cgaggtgttc acgaccacgg cgttgttcga cgtgacggcc
     1921 gcccgggctc acttcgacgg caccgtggag gccggggaat gccgtttctg cgcgtttgac
     1981 gcgcgcaacc gcgaacatca tgcgcggaac gccggaaggc tgttctagcg gccgtgtccg
     2041 cgcctctggg gcggttgcgc ctgccatggg tcgttctgcc actgttcggc ctcacgctgg
     2101 tctgtgcgct gcctgatctc cctgagcagg tcggccttgg tcctgggggc gcttcgctcc
     2161 tcgaacgggc cgctctcccc caggtcctcg ggctcgctca ggtccaacgg ctcgtcaccg
     2221 gacaggctcg ggccggttct ctccctgtgc cgggttctcc gcctgtgcgc gttgttctgg
     2281 ccatgcgact ctagaggatc tccttctgct cgtagcgatt acttcgagca ttactgacga
     2341 caaagacccc gaccgagatg gtcggggtct ttttgttgtg gtgctgtgac gtgttgtcca
     2401 accgtattat tccggactag ttcagcgctt ccataagctg ctcgaatccc agggtgggct
     2461 tggcctcgaa gggatcgagg agtatggctt cccggcgaga tctcgcgccg aagtaatcgc
     2521 tacgagcaga aggagatccc cgggtactga gctcgaattc tgagcaaaag gccagcaaaa
     2581 ggccagggac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2641 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2701 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2761 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2821 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2881 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2941 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3001 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3061 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3121 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3181 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3241 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3301 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3361 cttgggcgcg gcggccatga agtggcttga caagcataat cttgtctgat tcgtctattt
     3421 tcataccccc ttcggggaaa tagatgtgaa aacccttata aaacgcgggt tttcgcagaa
     3481 acatgcgcta gtatcattga tgacaacatg gactaagcaa aagtgcttgt cccctgaccc
     3541 aaggagatgc ttatgaactt taataaaatt gatttagaca attggaagag aaaagagata
     3601 tttaatcatt atttgaacca acaaacgact tttagtataa ccacagaaat tgatattagt
     3661 gttttatacc gaaacataaa acaagaagga tataaatttt accctgcatt tattttctta
     3721 gtgacaaggg tgataaactc aaatacagct tttagaactg gttacaatag cgacggagag
     3781 ttaggttatt gggataagtt agagccactt tatacaattt ttgatggtgt atctaaaaca
     3841 ttctctggta tttggactcc tgtaaagaat gacttcaaag agttttatga tttatacctt
     3901 tctgatgtag agaaatataa tggttcgggg aaattgtttc ccaaaacacc tatacctgaa
     3961 aatgcttttt ctctttctat tattccatgg acttcattta ctgggtttaa cttaaatatc
     4021 aataataata gtaattacct tctacccatt attacagcag gaaaattcat taataaaggt
     4081 aattcaatat atttaccgct atctttacag gtacatcatt ctgtttgtga tggttatcat
     4141 gcaggattgt ttatgaactc tattcaggaa ttgtcagata ggcctaatga ctggctttta
     4201 taatcgtctt caatctaaaa accaaaagcg ggcttgcgag cccgcttttt ttattacgtt
     4261 ccactgcaga gtactgagct caagcttgca ttgcaggcct gtcga
//
