LOCUS       V017042                 5773 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017042
VERSION     V017042
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(201..220)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     CDS             279..1424
                     /gene="galK"
                     /label="Galactokinase"
                     /note="Galactokinase from Escherichia coli O157:H7 (strain
                     EC4115 / EHEC). Accession#: B5YRF5"
     primer_bind     1811..1827
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(2071..2659)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2804..3249
                     /label="sacB promoter"
                     /note="sacB promoter and control region"
     CDS             3250..4668
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     CDS             complement(4958..5770)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
       61 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttccc cccccccgac
      121 gtcaggtggc tagaactaga tatccatgga tccagacccc aggcttgaca ctttatgctt
      181 ccggctcgta taatgtgtgg aattgtgagc gctcacaatt cgcatgcggt accaagctga
      241 tccgataaca caggaacaga tcttccggag tgtaagaaat gagtctgaaa gaaaaaacac
      301 aatctctgtt tgccaacgca tttggctacc ctgccactca caccattcag gcgcctggcc
      361 gcgtgaattt gattggtgaa cacaccgact acaacgacgg tttcgttctg ccctgcgcga
      421 ttgattatca aaccgtgatc agttgtgcac cacgcgatga ccgtaaagtt cgcgtgatgg
      481 cagccgatta tgaaaatcag ctcgacgagt tttccctcga tgcgcccatt gtcgcacatg
      541 aaaactatca atgggctaac tacgttcgtg gcgtggtgaa acatctgcaa ctgcgtaaca
      601 acagcttcgg cggcgtggac atggtgatca gcggcaatgt gccgcagggt gccgggttaa
      661 gttcttccgc ttcactggaa gtcgcggtcg gaaccgtatt gcagcagctt tatcatctgc
      721 cgctggacgg cgcacaaatc gcgcttaacg gtcaggaagc agaaaaccag tttgtaggct
      781 gtaactgcgg gatcatggat cagctaattt ccgcgctcgg caagaaagat catgccttgc
      841 tgatcgattg ccgctcactg gggaccaaag cagtttccat gcccaaaggt gtggctgtcg
      901 tcatcatcaa cagtaacttc aaacgtaccc tggttggcag cgaatacaac acccgtcgtg
      961 aacagtgcga aaccggtgcg cgtttcttcc agcagccagc cctgcgtgat gtcaccattg
     1021 aagagttcaa cgctgttgcg catgaactgg acccgatcgt ggcaaaacgc gtgcgtcata
     1081 tactgactga aaacgcccgc accgttgaag ctgccagcgc gctggagcaa ggcgacctga
     1141 aacgtatggg cgagttgatg gcggagtctc atgcctctat gcgcgatgat ttcgaaatca
     1201 ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc tgtgattggc gacaaaggtg
     1261 gcgtacgcat gaccggcggc ggatttggcg gctgtatcgt cgcgctgatc ccggaagaac
     1321 tggtgcctgc cgtacagcaa gctgtcgctg aacaatatga agcaaaaaca ggtattaaag
     1381 agacttttta cgtttgtaaa ccatcacaag gagcaggaca gtgctgaacg aaactcctgc
     1441 agcgcgtagt acctcacacc gtcctgcggg ttgacgtatg aggcttggat cctcccgctg
     1501 cgctgagtct tcagcgatcc ccatccgcga cgtgccaact aggtctcctc tcgtcgtgaa
     1561 caaggctacc gggttgcaac tcctgtgcaa ctctcaggct tcaacgcgct tctacgacct
     1621 gcaatttctt tccacttaga ggatgcagcc gagagggggt aaaaacctcc cattcgccat
     1681 tcaggctgcg caactgttgg gaagggcgtt tcggtgcggg cctcttcgct attacgccag
     1741 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     1801 tcacgacgtt gtaaaacgac ggccagtgag cgcgacgtaa taagttgaag agcatctgct
     1861 cttcagcatc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaacatg
     1921 gtcatagctg tttccttgcg tattgggcgc tctccgcttc ctcgctcact gactcgctgc
     1981 gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca aaaggccagc aaaaggccag
     2041 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2101 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2161 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2221 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2281 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2341 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2401 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2461 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2521 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2581 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2641 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2701 gaacgaaggc gcgcctgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2761 cgagtcagtg agcgaggaag cggaagatcg ggcccagccg gcccacatat acctgccgtt
     2821 cactattatt tagtgaaatg agatattatg atattttctg aattgtgatt aaaaaggcaa
     2881 ctttatgccc atgcaacaga aactataaaa aatacagaga atgaaaagaa acagatagat
     2941 tttttagttc tttaggcccg tagtctgcaa atccttttat gattttctat caaacaaaag
     3001 aggaaaatag accagttgca atccaaacga gagtctaata gaatgaggtc gaaaagtaaa
     3061 tcgcgcgggt ttgttactga taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt
     3121 atactttggc gtcacccctt acatatttta ggtctttttt tattgtgcgt aactaacttg
     3181 ccatcttcaa acaggagggc tggaagaagc agaccgctaa cacagtacat aaaaaaggag
     3241 acatgaacga tgaacatcaa aaagtttgca aaacaagcaa cagtattaac ctttactacc
     3301 gcactgctgg caggaggcgc aactcaagcg tttgcgaaag aaacgaacca aaagccatat
     3361 aaggaaacat acggcatttc ccatattaca cgccatgata tgctgcaaat ccctgaacag
     3421 caaaaaaatg aaaaatataa agttcctgag ttcgattcgt ccacaattaa aaatatctct
     3481 tctgcaaaag gcctggacgt ttgggacagc tggccattac aaaacactga cggcactgtc
     3541 gcaaactatc acggctacca catcgtcttt gcattagccg gagatcctaa aaatgcggat
     3601 gacacatcga tttacatgtt ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa
     3661 aacgctggcc gcgtctttaa agacagcgac aaattcgatg caaatgattc tatcctaaaa
     3721 gaccaaacac aagaatggtc aggttcagcc acatttacat ctgacggaaa aatccgttta
     3781 ttctacactg atttctccgg taaacattac ggcaaacaaa cactgacaac tgcacaagtt
     3841 aacgtatcag catcagacag ctctttgaac atcaacggtg tagaggatta taaatcaatc
     3901 tttgacggtg acggaaaaac gtatcaaaat gtacagcagt tcatcgatga aggcaactac
     3961 agctcaggcg acaaccatac gctgagagat cctcactacg tagaagataa aggccacaaa
     4021 tacttagtat ttgaagcaaa cactggaact gaagatggct accaaggcga agaatcttta
     4081 tttaacaaag catactatgg caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt
     4141 ctgcaaagcg ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg tatgattgag
     4201 ctaaacgatg attacacact gaaaaaagtg atgaaaccgc tgattgcatc taacacagta
     4261 acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta cctgttcact
     4321 gactcccgcg gatcaaaaat gacgattgac ggcattacgt ctaacgatat ttacatgctt
     4381 ggttatgttt ctaattcttt aactggccca tacaagccgc tgaacaaaac tggccttgtg
     4441 ttaaaaatgg atcttgatcc taacgatgta acctttactt actcacactt cgctgtacct
     4501 caagcgaaag gaaacaatgt cgtgattaca agctatatga caaacagagg attctacgca
     4561 gacaaacaat caacgtttgc gcctagcttc ctgctgaaca tcaaaggcaa gaaaacatct
     4621 gttgtcaaag acagcatcct tgaacaagga caattaacag ttaacaaata aaaacgcaaa
     4681 agaaaatgcc gatatcctat tggcattgcg gccgcggtct cggcggtggg tcgtctagag
     4741 aaagtgaggg agccacggtt ggtgagagct ttgttgtagg tggaccagtt ggtgattttg
     4801 aacttttgct ttgccacgga atggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc
     4861 aactcagcaa aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc
     4921 tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat
     4981 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     5041 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     5101 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     5161 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagat
     5221 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     5281 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     5341 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     5401 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     5461 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     5521 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     5581 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatgg ggcttcccat
     5641 acaagcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     5701 ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa
     5761 tatggctcat aac
//
