LOCUS       40924_26904       14268 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pKM24KHibm10, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14268)
  AUTHORS   Sahoo DK, Raha S, Hall JT, Maiti IB.
  TITLE     Overexpression of the synthetic chimeric native-T-phylloplanin-GFP 
            genes optimized for monocot and dicot plants renders enhanced 
            resistance to blue mold disease in tobacco (N. tabacum L.)
  JOURNAL   ScientificWorldJournal 2014, 601314 (2014)
  PUBMED    24778589
REFERENCE   2  (bases 1 to 14268)
  AUTHORS   Maiti IB, Sahoo DK, Raha S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2013) KTRDC, Plant Genetic Engineering Research 
            and Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14268)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14268)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "ScientificWorldJournal 2014, 601314 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2013) KTRDC, Plant Genetic Engineering Research and 
            Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..641
                     /note="M24 promoter from Mirabilis Mosaic Virus (MMV), Ref:
                     US Patent No. 64205470; Plant Mol. Biol. 40: 771-782 
                     (1999); Transgenics 3: 61-70 (1999)"
                     /regulatory_class="promoter"
     regulatory      642..691
                     /note="5'-untranslated region of Alfalfa mosaic virus
                     (AlMV) RNA 4. ref: Nature 325: 622-625(1987)"
                     /regulatory_class="enhancer"
     sig_peptide     692..760
                     /gene="fused T-phylloplanin-GFP"
                     /note="apoplast/cell wall/plasma membrane targeting protein
                     sequence (aTPS) of Arabidopsis 2S2 protein gene. Ref: Plant
                     Physiol. 155: 2036-2048 (2011); Proc Natl Acad Sci, USA, 
                     104: 1430-1435 (2007)"
     misc_feature    761..1213
                     /gene="fused T-phylloplanin-GFP"
                     /note="synthetic T-phylloplanin sequence, (GenBank
                     accession No. AY705384), full-length with its own signal 
                     peptide, is optimized for monocot plant (brent grass) bias 
                     codons"
     misc_feature    1214..1261
                     /gene="fused T-phylloplanin-GFP"
                     /note="linker sequence used to fuse T-Phylloplanin and GFP
                     genes in frame"
     CDS             1268..1978
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     terminator      2003..2644
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        2733..2912
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2940..3959
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      3971..4220
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5111..5929)
                     /label=KanR
                     /note="fusion between an N-terminal peptide of nopaline
                     synthase and Tn5 aminoglycoside phosphotransferase"
     promoter        complement(5930..6113)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6269..6293
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6773..7482
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(8479..9126)
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     CDS             9232..10428
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             complement(11707..12852)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(13127..13495)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(13528..13637)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_feature    14192..14216
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaattcgtca acttcgtcca cagacatcaa catcttatcg tcctttgaag ataagataat
       61 aatgttgaag ataagagtgg gagcccccac taaaacattg ctttgtcaaa agctaaaaaa
      121 gatgatgccc gacagccact tgtgtgaagc atgagaagcc ggtccctcca ctaagaaaat
      181 tagtgaagca tcttccagtg gtccctccac tcacagctca atcagtgagc aacaggacga
      241 aggaaatgac gtaagccatg acgtctaatc ccaacttcgt ccacagacat caacatctta
      301 tcgtcctttg aagataagat aataatgttg aagataagag tgggagccac cactaaaaca
      361 ttgctttgtc aaaagctaaa aaagatgatg cccgacagcc acttgtgtga agcatgagaa
      421 gccggtccct ccactaagaa aattagtgaa gcatcttcca gtggtccctc cactcacagc
      481 tcaatcagtg agcaacagga cgaaggaaat gacgtaagcc atgacgtcta atcccacaag
      541 aatttcctta tataaggaac acaaatcaga aggaagagat caatcgaaat caaaatcgga
      601 atcgaaatca aaatcggaat cgaaatctct catctaagct tggatcctcg agcggatctt
      661 tttattttta attttctttc aaatacttcc aatgggcgcc aacaagctct tcctcgtgtg
      721 cgccaccttc gccctctgct tcctcctcac caacgccccc atgggtgcca gcgccaagat
      781 cttcctcatc ttcctgctcg ccgccctgat cgccaccccg gccgcgttcg cgatcctcgt
      841 gccgaccctc gtgtccaccc acatcagcgg cctcgtgttc tgctccgtga acggcaacct
      901 cgacgtgatc aacggcctca gcccgcaggt gttcccgaac gccagcgtcc agctccgctg
      961 cggcgccacc aacgtgatca gcagcaccat caccaacggc agcggcgcct tcagcctcgc
     1021 cgtgaacacc ttcccgctcc tcaactgcaa cctcgtggtg gccaccccgc tctccacctg
     1081 caacgccacc ctccagtctg tgggccgcct cgccagcagc ctccgcctcg tgaacatcac
     1141 cctcggcagc ggcaccggcc tcatccgtgt gggcctcgcc ccgaccggct tcatcctcaa
     1201 cctcaacatc aacgccatcg gcccgatgta caaccagtac cagtacccga acgggtcgac
     1261 tatgggcagc aagggcgagg agctgttcac cggcgtggtc ccgatcctcg tggaactcga
     1321 cggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg acgccaccta
     1381 cggcaagctc accctcaagt tcatctgcac caccggcaag ctcccggtgc cgtggccgac
     1441 cctggtgacc accttcagct acggcgtcca gtgcttcagc cgctacccgg accacatgaa
     1501 gcagcacgac ttcttcaaga gcgccatgcc cgagggctac gtccaggagc gcaccatctt
     1561 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1621 cgtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctcggcca
     1681 caagctggag tacaactaca acagccacaa cgtgtacatc atggccgaca agcagaagaa
     1741 cggcatcaag gtcaacttca agatccgcca caacatcgag gacggctccg tccagctcgc
     1801 cgaccactac cagcagaaca ccccgatcgg cgacggcccg gtgctcctcc cggacaacca
     1861 ctacctcagc acccagagcg ccctcagcaa ggacccgaac gagaagcgcg accacatggt
     1921 gctgctggag ttcgtgaccg ccgccggcat cacccacggc atggacgagc tgtacaagtg
     1981 agagctcgaa gtgatcctct agagctttcg ttcgtatcat cggtttcgac aacgttcgtc
     2041 aagttcaatg catcagtttc attgcgcaca caccagaatc ctactgagtt tgagtattat
     2101 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     2161 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     2221 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     2281 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     2341 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta
     2401 tgcttattca ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga
     2461 atacaagtat gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca
     2521 gaatccttgt cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag
     2581 ttgagtatga aaatattttt taatgcattt tatgacttgc caattgattg acaacatgca
     2641 tcaatcgatg atcatgagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg
     2701 cgggacaagc cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactcag
     2761 ccgcgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt
     2821 caaaagtcgc ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg
     2881 acgttccata aattcccctc ggtatccaat tagagtctca tattcactct caagatatca
     2941 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     3001 gcgtctccga ccagatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     3061 taagagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggttcc tacaaagatc
     3121 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     3181 gggagtttag cgagagcctg acctattgca tctcccgccg ttcacagggt gtcacgttgc
     3241 aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag gctatggatg
     3301 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     3361 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     3421 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     3481 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     3541 ccaacaatgt cctgacggac aacggccgca taacagcggt cattgactgg agcgaggcga
     3601 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     3661 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccac
     3721 gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     3781 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     3841 ccgggactgt cgggcgaaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     3901 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     3961 gatagggatc catcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     4021 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     4081 catgtaatgc atgacgttgt ttatgagatg ggtttttatg attagagtcc cgcaattata
     4141 catttaatac acgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     4201 ggtgtcatct atgttactag taatcgatcg atctagtaac atagatgaca ccgcgcgcga
     4261 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
     4321 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
     4381 tacatgctta acgtaatcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     4441 ttcaatttta agaaacttta ttgcccaaat ggttttgacg atctgcttcg acgcactcct
     4501 tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac gaatcaagtc
     4561 gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa tcttgccttt
     4621 ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt tggcaatagc
     4681 tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc gatgttccat
     4741 gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt attccctcaa
     4801 agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat tgagacgcaa
     4861 tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga cgttgcaaac
     4921 gtaagtgttg gatcggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     4981 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     5041 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga
     5101 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     5161 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     5221 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     5281 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
     5341 gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
     5401 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     5461 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     5521 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     5581 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     5641 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     5701 atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
     5761 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     5821 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgccc
     5881 ggatccgggc ggaaataggt aaagaagttg cggataaggt aattgccatt gcagattatt
     5941 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     6001 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     6061 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg
     6121 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     6181 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcag attgtcgttt
     6241 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     6301 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     6361 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggagtgcttg gcattccgtg
     6421 cgataatgac ttctgttcaa ccacccaaac gtcggaaagc ctgacgacgg agcagcattc
     6481 caaaaagatc ccttggctcg tctgggtcgg ctagaaggtc gagtgggctg ctgtggcttg
     6541 atccctcaac gcggtcgcgg acgtagcgca gcgccgaaaa atcctcgatc gcaaatccga
     6601 cgctgtcgaa aagcgtgatc tgcttgtcgc tctttcggcc gacgtcctgg ccagtcatca
     6661 cgcgccaaag ttccgtcaca ggatgatctg gcgcgagttg ctggatctcg ccttcaatcc
     6721 gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag caagatcgtc gaccctttcc
     6781 gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa
     6841 cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc gttgtggata
     6901 cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc
     6961 cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg
     7021 gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat
     7081 gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac
     7141 tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc
     7201 gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt
     7261 ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat
     7321 aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg
     7381 tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc
     7441 tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagcta gcttgggctg
     7501 aaattgcagg acaactggtg agtctggatc tgaagttcat tgttgtcatg gatgatctgg
     7561 accgactgga gccatcccag gtggcggaag tgttcaggct tgtgcgtgca gtagccgatc
     7621 tgccccgctt tacccatatt ctctgttatg acaggcagat tatcactcat gccgttgaac
     7681 atgcgctgaa tatcgaagat ggcagccgtt atctccagaa aatcattcag cttagtttta
     7741 aattaccccg acctgaagcc tttgatttac gtaatgaatt tcgccagcgg gctgaggctc
     7801 tatatcagca aattaataat caaccgccag actctggaat ggtaagggat ctcatcgcgg
     7861 tgactgatac ctatggtgcc gcactttcga cgccacggga aatccatcag gccattaatt
     7921 ccctgatttt tctttatccg gggatgcggg attttgttta tttccctgat ttgtgcctgc
     7981 ttcagcttat acgggtgaca aacccggctc tgtatgactg gacagagcat tacctgacag
     8041 aacggtccgt gattgaaacc ggtcagggta tgctttctga cggagagaaa gcagacttcc
     8101 gggaggggct tatcagatgt atgaagacgt tcagggcatc aaatgcagac tcgtttctga
     8161 cacttgcaga ctggatctat ctcatctgcg caaggcagaa cgtgaagacg gccgccctgg
     8221 acctcgcccg cgagcgccag gcgcacgagg ccggcgcgcg gacccgcgcc acggcccacg
     8281 agcggacgcc gcagcaggag cgccagaagg ccgccagaga ggccgagcgc ggccgtgagg
     8341 cttggacgct agggcagggc atgaaaaagc ccgtagcggg ctgctacggg cgtctgacgc
     8401 ggtggaaagg gggaggggat gttgtctaca tggctctgct gtagtgagtg ggttgcgctc
     8461 cggcagcggt cctgatcaat cgtcaccctt tctcggtcct tcaacgttcc tgacaacgag
     8521 cctccttttc gccaatccat cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg
     8581 accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc
     8641 aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc
     8701 aacagtgaag tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc
     8761 ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt
     8821 gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc
     8881 attcccgatc agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt
     8941 ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca
     9001 gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc
     9061 tacgccgacc tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt
     9121 tgtcatgctt gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata
     9181 aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga catgaaaccc
     9241 aacatacccc tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg
     9301 attatgccgg tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc
     9361 cactatggca ttctgctggc gctgtatgcg ttggtgcaat ttgcctgcgc acctgtgctg
     9421 ggcgcgctgt cggatcgttt cgggcggcgg ccaatcttgc tcgtctcgct ggccggcgcc
     9481 actgtcgact acgccatcat ggcgacagcg cctttccttt gggttctcta tatcgggcgg
     9541 atcgtggccg gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatatc
     9601 actgatggcg atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt cgggttcggg
     9661 atggtcgcgg gacctgtgct cggtgggctg atgggcggtt tctcccccca cgctccgttc
     9721 ttcgccgcgg cagccttgaa cggcctcaat ttcctgacgg gctgtttcct tttgccggag
     9781 tcgcacaaag gcgaacgccg gccgttacgc cgggaggctc tcaacccgct cgcttcgttc
     9841 cggtgggccc ggggcatgac cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa
     9901 cttgtcggac aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg
     9961 gacgcgacca cgatcggcat ttcgcttgcc gcatttggca ttctgcattc actcgcccag
    10021 gcaatgatca ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact catgctcgga
    10081 atgattgccg acggcacagg ctacatcctg cttgccttcg cgacacgggg atggatggcg
    10141 ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg
    10201 ttgtccaggc aggtggatga ggaacgtcag gggcagctgc aaggctcact ggcggcgctc
    10261 accagcctga cctcgatcgt cggacccctc ctcttcacgg cgatctatgc ggcttctata
    10321 acaacgtgga acgggtgggc atggattgca ggcgctgccc tctacttgct ctgcctgccg
    10381 gcgctgcgtc gcgggctttg gagcggcgca gggcaacgag ccgatcgctg atcgtggaaa
    10441 cgataggcct atgccatgcg ggtcaaggcg acttccggca agctatacgc gccctaggag
    10501 tgcggttgga acgttggccc agccagatac tcccgatcac gagcaggacg ccgatgattt
    10561 gaagcgcact cagcgtctga tccaagaaca accatcctag caacacggcg gtccccgggc
    10621 tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg cgagatcccc cggaaccaaa
    10681 ggaagtaggt taaacccgct ccgatcaggc cgagccacgc caggccgaga acattggttc
    10741 ctgtaggcat cgggattggc ggatcaaaca ctaaagctac tggaacgagc agaagtcctc
    10801 cggccgccag ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc cacttgcggg
    10861 tcagcacggt tccgaacgcc atggaaaccg cccccgccag gcccgctgcg acgccgacag
    10921 gatctagcgc tgcgtttggt gtcaacacca acagcgccac gcccgcagtt ccgcaaatag
    10981 cccccaggac cgccatcaat cgtatcgggc tacctagcag agcggcagag atgaacacga
    11041 ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg
    11101 aaataaacaa caagctccag aatagcgaaa tattaagtgc gccgaggatg aagatgcgca
    11161 tccaccagat tcccgttgga atctgtcgga cgatcatcac gagcaataaa cccgccggca
    11221 acgcccgcag cagcataccg gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc
    11281 cggacagatg cgccttgtga gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc
    11341 caatgatctc gccgtcgatg taggcgccga atgccacggc atctcgcaac cgttcagcga
    11401 acgcctccat gggctttttc tcctcgtgct cgtaaacgga cccgaacatc tctggagctt
    11461 tcttcagggc cgacaatcgg atctcgcgga aatcctgcac gtcggccgct ccaagccgtc
    11521 gaatctgagc cttaatcaca attgtcaatt ttaatcctct gtttatcggc agttcgtaga
    11581 gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg
    11641 ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag
    11701 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct
    11761 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga
    11821 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga
    11881 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat
    11941 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg
    12001 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga
    12061 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag
    12121 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag
    12181 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac
    12241 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt
    12301 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt
    12361 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc
    12421 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg
    12481 tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt
    12541 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc
    12601 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc
    12661 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga
    12721 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc
    12781 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa
    12841 cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc
    12901 ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat
    12961 gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc
    13021 ctgcacgaat accagctccg cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt
    13081 ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa aagtcatggc tctgccctcg
    13141 ggcggaccac gcccatcatg accttgccaa gctcgtcctg cttctcttcg atcttcgcca
    13201 gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga
    13261 gtttcagcag gccgcccagg cggcccaggt cgccattgat gcgggccagc tcgcggacgt
    13321 gctcatagtc cacgacgccc gtgattttgt agccctggcc gacggccagc aggtaggccg
    13381 acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc
    13441 agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct
    13501 tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga
    13561 gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta
    13621 cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc
    13681 tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa
    13741 tgaccccgaa gcagggttat gcagcggaaa agatccgtcg acgatcgtca acgttcactt
    13801 ctaaagaaat agcgccactc agcttcctca gcggctttat ccagcgattt cctattatgt
    13861 cggcatagtt ctcaagatcg acagcctgtc acggttaagc gagaaatgaa taagaaggct
    13921 gataattcgg atctctgcga gggagatgat atttgatcac aggcagcaac gctctgtcat
    13981 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
    14041 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
    14101 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
    14161 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
    14221 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtact
//
