LOCUS       40924_26894       10975 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pKM244, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Mertens K, Berg JP, Brennan RE, Weber MM, Chen C, Samuel JE.
  TITLE     Evolutionary Loss of katE as Balance Mechanism for Antioxidant Gene 
            Expression in Coxiella burnetii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   Mertens K, Berg JP, Brennan RE, Weber MM, Chen C, Samuel JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2010) Microbial and Molecular Pathogenesis, Texas 
            A
REFERENCE   3  (bases 1 to 10975)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10975)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-OCT-2010) Microbial and Molecular Pathogenesis, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             635..1342
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             1632..2288
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      2532..2618
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2710..2737
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2756..2847
                     /label=AmpR promoter
     CDS             2848..3705
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(4145..4539)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             6081..7049
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             7563..8399
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             8389..9237
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     misc_feature    10000..10056
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(10599..10676)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        10906..10934
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    10942..10958
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttatga
       61 taagatcgac agggcaatga gggttgattt tggtcacatc ccctttcatg cgcgccatcg
      121 catcgcgcat cgcggctaaa tcgaccacgg cgggaacgcc ggtaaaatct tgcattaaga
      181 ctcgcgcggg tcgataagcg atttcgcggt cggaatgttt atctttcagc cagtgagcga
      241 acgcttcaat atgcgtttga gtgactgttt ctccatcttc atgccgtaat tggttttcca
      301 ataaaatttt gagtgagtac ggaaggcgat gaatgtttga aagacccgcg tcttccgccg
      361 cttttaagct atggtagtgg taggttttgc caccagccgt taattcacgc cgtgttttta
      421 atgaatccgc catcgttacc tcgcaaccaa ttttgcgctt gagcttaaca cattcgaaag
      481 tgcgacagaa gtcggtcaag ccgcaaccgg ccatgttatg acgaagaaat taaaaatcgc
      541 ccttgaattt tcatcacctg cccttatatg actagtgatt gtcgctcatg ttattgagct
      601 tggtaatttt acaaataaat ggagagggtt acatatggtg agcaagggcg aggaggataa
      661 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      721 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      781 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      841 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      901 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      961 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     1021 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     1081 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     1141 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     1201 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     1261 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     1321 cggcatggac gagctgtaca agtaatctag atgcagaaga caaacgttac aacccagccc
     1381 tgcaattgga acgaagcaat tgggccgatg atcaggagca tcgattgcac aacgcaatgc
     1441 aatcgatgga tgatcgaagt cgagatattt atatcagcgt tggttaagtg aaaaaaaatc
     1501 aacgcttcat gaattagcag cacaatacgc tgtttctgcc gaacgtattc ggcagcttga
     1561 aaaaaacgcc atgcagaaaa ttcgaactgc tatggcagat gaataatttt taactctaag
     1621 gggaatagca tatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     1681 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     1741 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     1801 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
     1861 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     1921 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     1981 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     2041 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     2101 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     2161 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg
     2221 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     2281 gcggggcgta atttttttaa ggcagttatt ggtgccctta aacaagcttg gctgttttgg
     2341 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     2401 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     2461 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     2521 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2581 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     2641 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     2701 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt
     2761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2821 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2881 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2941 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3001 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3061 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     3121 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3181 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3241 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3301 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3361 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3421 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3481 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3541 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3601 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3661 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3721 gtttactcat atatacttta gattgatttc tgaaagcgac caggtgctcg gcgtggcaag
     3781 actcgcagcg aacccgtaga aagccatgct ccagccgccc gcattggaga aattcttcaa
     3841 attcccgttg cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg
     3901 ccgggtaata ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt
     3961 aacgaccagt atcccgatcc cggctggccg tcctggccgc cacatgaggc atgttccgcg
     4021 tccttgcaat actgtgttta catacagtct atcgcttagc ggaaagttct tttaccctca
     4081 gccgaaatgc ctgccgttgc tagacattgc cagccagtgc ccgtcactcc cgtactaact
     4141 gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat aactgtcacg aacccctgca
     4201 ataactgtca cgcccccaaa cctgcaaacc cagcaggggc gggggctggc ggggtgttgg
     4261 aaaaatccat ccatgattat ctaagaataa tccactaggc gcggttatca gcgcccttgt
     4321 ggggcgctgc tgcccttgcc caatatgccc ggccagaggc cggatagctg gtctattcgc
     4381 tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg
     4441 ctaaacccca caccaaaccc cgcagaaata cgctggagcg cttttagccg ctttagcggc
     4501 ctttccccct acccgaaggg tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg
     4561 atcattcagc ccggctcatc cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg
     4621 tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat cctccggcca ctcgctgctg
     4681 ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc gccttgtttc gttcttgcgc
     4741 tcttgctgct gttcccttgc ccgctcccgc tgaatttcgg cattgattcg cgctcgttgt
     4801 tcttcgagct tggccagccg atccgccgcc ttgttgctcc ccttaaccat cttgacaccc
     4861 cattgttaat gtgctgtctc gtaggctatc atggcgaaaa gcaaaagcaa cagcgaggca
     4921 gcatggcgat ttatcacctt acggcgaaaa ccggcagcag gtcgggcggc caatcggcca
     4981 gggccaaggc cgactacatc cagcgcgaag gcaagtatgc ccgcgacatg gatgaagtct
     5041 tgcacgccga atccgggcac atgccggagt tcgtcgagcg gcccgccgac tactgggatg
     5101 ctgccgacct gtatgaacgc gccaatgggc ggctgttcaa ggaggtcgaa tttgccctgc
     5161 cggtcgagct gaccctcgac cagcagaagg cgctggcgtc cgagttcgcc cagcacctga
     5221 ccggtgccga gcgcctgccg tatacgctgg ccatccatgc cggtggcggc gagaacccgc
     5281 actgccacct gatgatctcc gagcggatca atgacggcat cgagcggccc gccgctcagt
     5341 ggttcaagcg gtacaacggc aagaccccgg agaagggcgg ggcacagaag accgaagcgc
     5401 tcaagcccaa ggcatggctt gagcagaccc gcgaggcatg ggccgaccat gccaaccggg
     5461 cattagagcg ggctggccac gacgcccgca ttgaccacag aacacttgag gcgcagggca
     5521 tcgagcgcct gcccggtgtt cacctggggc cgaacgtggt ggagatggaa ggccggggca
     5581 tccgcaccga ccgggcagac gtggccctga acatcgacac cgccaacgcc cagatcatcg
     5641 acttacagga ataccgggag gcaatagacc atgaacgcaa tcgacagagt gaagaaatcc
     5701 agaggcatca acgagttagc ggagcagatc gaaccgctgg cccagagcat ggcgacactg
     5761 gccgacgaag cccggcaggt catgagccag accaagcagg ccagcgaggc gcaggcggcg
     5821 gagtggctga aagcccagcg ccagacaggg gcggcatggg tggagctggc caaagagttg
     5881 cgggaggtag ccgccgaggt gagcagcgcc gcgcagagcg cccggagcgc gtcgcggggg
     5941 tggcactgga agctatggct aaccgtgatg ctggcttcca tgatgcctac ggtggtgctg
     6001 ctgatcgcat cgttgctctt gctcgacctg acgccactga caaccgagga cggctcgatc
     6061 tggctgcgct tggtggcccg atgaagaacg acaggacttt gcaggccata ggccgacagc
     6121 tcaaggccat gggctgtgag cgcttcgata tcggcgtcag ggacgcaccc accggccaga
     6181 tgatgaaccg ggaatggtca gccgccgaag tgctccagaa cacgccatgg ctcaagcgga
     6241 tgaatgccca gggcaatgac gtgtatatca ggcccgccga gcaggagcgg catggtctgg
     6301 tgctggtgga cgacctcagc gagtttgacc tggatgacat gaaagccgag ggccgggagc
     6361 ctgccctggt agtggaaacc agcccgaaga actatcaggc atgggtcaag gtggccgacg
     6421 ccgcaggcgg tgaacttcgg gggcagattg cccggacgct ggccagcgag tacgacgccg
     6481 acccggccag cgccgacagc cgccactatg gccgcttggc gggcttcacc aaccgcaagg
     6541 acaagcacac cacccgcgcc ggttatcagc cgtgggtgct gctgcgtgaa tccaagggca
     6601 agaccgccac cgctggcccg gcgctggtgc agcaggctgg ccagcagatc gagcaggccc
     6661 agcggcagca ggagaaggcc cgcaggctgg ccagcctcga actgcccgag cggcagctta
     6721 gccgccaccg gcgcacggcg ctggacgagt accgcagcga gatggccggg ctggtcaagc
     6781 gcttcggtca tgacctcagc aagtgcgact ttatcgccgc gcagaagctg gccagccggg
     6841 gccgcagtgc cgaggaaatc ggcaaggcca tggccgaggc cagcccagcg ctggcagagc
     6901 gcaagcccgg ccacgaagcg gattacatcg agcgcaccgt cagcaaggtc atgggtctgc
     6961 ccagcgtcca gcttgcgcgg gccgagctgg cacgggcacc ggcaccccgc cagcgaggca
     7021 tggacagggg cgggccagat ttcagcatgt agtgcttgcg ttggtactca cgcctgttat
     7081 actatgagta ctcacgcaca gaagggggtt ttatggaata cgaaaaaagc gcttcagggt
     7141 cggtctacct gatcaaaagt gacaagggct attggttgcc cggtggcttt ggttatacgt
     7201 caaacaaggc cgaggctggc cgcttttcag tcgctgatat ggccagcctt aaccttgacg
     7261 gctgcacctt gtccttgttc cgcgaagaca agcctttcgg ccccggcaag tttctcggtg
     7321 actgatatga aagaccaaaa ggacaagcag accggcgacc tgctggccag ccctgacgct
     7381 gtacgccaag cgcgatatgc cgagcgcatg aaggccaaag ggatgcgtca gcgcaagttc
     7441 tggctgaccg acgacgaata cgaggcgctg cgcgagtgcc tggaagaact cagagcggcg
     7501 cagggcgggg gtagtgaccc cgccagcgcc taaccaccaa ctgcctgcaa aggaggcaat
     7561 caatggctac ccataagcct atcaatattc tggaggcgtt cgcagcagcg ccgccaccgc
     7621 tggactacgt tttgcccaac atggtggccg gtacggtcgg ggcgctggtg tcgcccggtg
     7681 gtgccggtaa atccatgctg gccctgcaac tggccgcaca gattgcaggc gggccggatc
     7741 tgctggaggt gggcgaactg cccaccggcc cggtgatcta cctgcccgcc gaagacccgc
     7801 ccaccgccat tcatcaccgc ctgcacgccc ttggggcgca cctcagcgcc gaggaacggc
     7861 aagccgtggc tgacggcctg ctgatccagc cgctgatcgg cagcctgccc aacatcatgg
     7921 ccccggagtg gttcgacggc ctcaagcgcg ccgccgaggg ccgccgcctg atggtgctgg
     7981 acacgctgcg ccggttccac atcgaggaag aaaacgccag cggccccatg gcccaggtca
     8041 tcggtcgcat ggaggccatc gccgccgata ccgggtgctc tatcgtgttc ctgcaccatg
     8101 ccagcaaggg cgcggccatg atgggcgcag gcgaccagca gcaggccagc cggggcagct
     8161 cggtactggt cgataacatc cgctggcagt cctacctgtc gagcatgacc agcgccgagg
     8221 ccgaggaatg gggtgtggac gacgaccagc gccggttctt cgtccgcttc ggtgtgagca
     8281 aggccaacta tggcgcaccg ttcgctgatc ggtggttcag gcggcatgac ggcggggtgc
     8341 tcaagcccgc cgtgctggag aggcagcgca agagcaaggg ggtgccccgt ggtgaagcct
     8401 aagaacaagc acagcctcag ccacgtccgg cacgacccgg cgcactgtct ggcccccggc
     8461 ctgttccgtg ccctcaagcg gggcgagcgc aagcgcagca agctggacgt gacgtatgac
     8521 tacggcgacg gcaagcggat cgagttcagc ggcccggagc cgctgggcgc tgatgatctg
     8581 cgcatcctgc aagggctggt ggccatggct gggcctaatg gcctagtgct tggcccggaa
     8641 cccaagaccg aaggcggacg gcagctccgg ctgttcctgg aacccaagtg ggaggccgtc
     8701 accgctgaat gccatgtggt caaaggtagc tatcgggcgc tggcaaagga aatcggggca
     8761 gaggtcgata gtggtggggc gctcaagcac atacaggact gcatcgagcg cctttggaag
     8821 gtatccatca tcgcccagaa tggccgcaag cggcaggggt ttcggctgct gtcggagtac
     8881 gccagcgacg aggcggacgg gcgcctgtac gtggccctga accccttgat cgcgcaggcc
     8941 gtcatgggtg gcggccagca tgtgcgcatc agcatggacg aggtgcgggc gctggacagc
     9001 gaaaccgccc gcctgctgca ccagcggctg tgtggctgga tcgaccccgg caaaaccggc
     9061 aaggcttcca tagatacctt gtgcggctat gtctggccgt cagaggccag tggttcgacc
     9121 atgcgcaagc gccgcaagcg ggtgcgcgag gcgttgccgg agctggtcgc gctgggctgg
     9181 acggtaaccg agttcgcggc gggcaagtac gacatcaccc ggcccaaggc ggcaggctga
     9241 ccccccccac tctattgtaa acaagacatt tttatctttt atattcaatg gcttattttc
     9301 ctgctaattg gtaataccat gaaaaatacc atgctcagaa aaggcttaac aatattttga
     9361 aaaattgcct actgagcgct gccgcacagc tccataggcc gctttcctgg ctttgcttcc
     9421 agatgtatgc tcttctgctc ccgaacgcca gcaagacgta gcccagcgcg tcggccagct
     9481 tgcaattcgc gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9601 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     9661 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg tggtttgccc cagcaggcga
     9721 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     9781 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     9841 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     9901 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     9961 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
    10021 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
    10081 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
    10141 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
    10201 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
    10261 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
    10321 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
    10381 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
    10441 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
    10501 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
    10561 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
    10621 tcttccgggc gctatcatgc cataccgcga aaggttttgc accattcgat ggtgtcaacg
    10681 taaatgccgc ttcgccttcg cgcgcgaatt gcaagctgat ccgggcttat cgactgcacg
    10741 gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc
    10801 gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt
    10861 gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat
    10921 cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga aacag
//
