LOCUS       40924_26884        9988 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pKLMF-FX, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Ganatra MB, Read JD, Colussi PA, Taron CH.
  TITLE     Vectors for intracellular expression of maltose binding protein 
            fusions in the yeast Kluyveromyces lactis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   Ganatra MB, Read JD, Colussi PA, Taron CH.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2008) Gene Expression Division, New England 
            Biolabs, 240 County Road, Ipswich, MA 01938, USA
REFERENCE   3  (bases 1 to 9988)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9988)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-AUG-2008) Gene Expression Division, New England Biolabs, 240 
            County Road, Ipswich, MA 01938, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..1113
                     /codon_start=1
                     /label=MBP
                     /note="maltose binding protein from E. coli"
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             1126..1137
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     misc_feature    1138..1234
                     /label=multiple cloning site
                     /note="multiple cloning site"
     terminator      1252..1870
                     /label=LAC4 terminator
                     /note="transcription terminator for Kluyveromyces lactis
                     LAC4"
     promoter        1871..2573
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             2594..4237
                     /codon_start=1
                     /label=amdS
                     /note="acetamidase from Aspergillus nidulans"
                     /translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGIL
                     SEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALAQA
                     RELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRK
                     AGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVG
                     TDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFT
                     KSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPPILRGVE
                     TTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAIPNIKDLL
                     NPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPTAAVRHDQF
                     RYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYDPEAYHGAPV
                     AVQVIGRRLSEERTLAIAEEVGKLLGNVVTP"
     promoter        4876..5532
                     /label=LAC4 promoter (5')
                     /note="5' portion of the Kluyveromyces lactis LAC4
                     promoter"
     promoter        complement(5549..5567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5581..5597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5605..5621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5629..5659)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5674..5695)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5983..6571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6745..7602)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7603..7707)
                     /label=AmpR promoter
     rep_origin      7734..8189
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8330..8346
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8360..9888
                     /label=LAC4 promoter (3')
                     /note="3' portion of a modified Kluyveromyces lactis LAC4 
                     promoter"
     protein_bind    9894..9910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aagcttgcca ccatgaaaac tgaagaaggt aaactggtaa tctggattaa cggcgataaa
       61 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
      121 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
      181 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
      241 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
      301 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg
      361 atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg
      421 gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac
      481 ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag
      541 tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg
      601 gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct
      661 gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc
      721 gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc
      781 aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg
      841 gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa
      901 gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca
      961 cgtattgccg ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag
     1021 atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag
     1081 actgtcgatg aagccctgaa agacgcgcag actaattcga gctcgatcga gggaaggctc
     1141 gagaaaagag aggctgaagc tagaagagct catatgtcca tgggcggccg cgatatcgtc
     1201 gacggatccg aattccctgc aggtaattaa ataaaggcct tgaatcgaga atttatactt
     1261 agataagtat gtacttacag gtatatttct atgagatact gatgtataca tgcatgataa
     1321 tatttaaacg gttattagtg ccgattgtct tgtgcgataa tgacgttcct atcaaagcaa
     1381 tacacttacc acctattaca tgggccaaga aaatattttc gaacttgttt agaatattag
     1441 cacagagtat atgatgttat ccgttagatt atgcatgatt cattcctaca actttttcgt
     1501 agcataagga ttaattactt ggatgccaat aaaaaaaaaa aacatcgaga aaatttcagc
     1561 atgctcagaa acaattgcag tgtatcaaag taaaaaaaag attttcacta catgttcctt
     1621 ttgaagaaag aaaatcatgg aacattagat ttacaaaaat ttaaccaccg ctgattaacg
     1681 attagaccgt taagcgcaca acaggttatt agtacagaga aagcattctg tggtgttgcc
     1741 ccggactttc ttttgcgaca taggtaaatc gaataccatc atactatctt ttccaatgac
     1801 tccctaaaga aagactcttc ttcgatgttg tatacgttgg agcatagggc aagaattgtg
     1861 gcttgagatc atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca
     1921 accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg
     1981 gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac
     2041 tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact gtagccctag
     2101 acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc catctattga
     2161 agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt
     2221 tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac
     2281 gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca
     2341 cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg
     2401 gccttccttc cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt
     2461 ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct
     2521 tccttgtttc tttttctgca caatatttca agctatacca agcatacaat caagcaattc
     2581 cagatctgcc accatgcctc aatcctggga agaactggcc gctgataagc gcgcccgcct
     2641 cgcaaaaacc atccctgatg aatggaaagt ccagacgctg cctgcggaag acagcgttat
     2701 tgatttccca aagaaatcgg gcatcctttc agaggccgaa ctgaagatca cagaggcttc
     2761 cgctgcggat cttgtgtcca agctggcggc cggagagttg acctcggtgg aagttacgct
     2821 agcattctgt aaacgggcag caatcgccca gcagttaaca aactgcgccc acgagttctt
     2881 ccctgacgcc gctctcgcgc aggcaaggga actcgatgaa tactacgcaa agcacaagag
     2941 acccgttggt ccacttcatg gcctccccat ctctctcaaa gaccagcttc gagtcaaggg
     3001 ctacgaaaca tcaatgggct acatctcatg gctaaacaag tacgacgaag gggactcggt
     3061 tctgacaacc atgctccgca aagccggtgc cgtcttctac gtcaagacct ctgtcccgca
     3121 gaccctgatg gtctgcgaga cagtcaacaa catcatcggg cgcaccgtca acccacgcaa
     3181 caagaactgg tcgtgcggcg gcagttctgg tggtgagggt gcgatcgttg ggattcgtgg
     3241 tggcgtcatc ggtgtaggaa cggacatcgg tggctcgatt cgagtgccgg ccgcgttcaa
     3301 cttcctgtac ggtctaaggc cgagtcatgg gcggctgccg tatgcaaaga tggcgaacag
     3361 catggagggt caggagacgg tgcacagcgt tgtcgggccg attacgcact ctgttgagga
     3421 cctccgcctc ttcaccaaat ccgtcctcgg tcaggagcct tggaaatacg actccaaggt
     3481 catccccatg ccctggcgcc agtccgagtc ggacattatt gcctccaaga tcaagaacgg
     3541 cgggctcaat atcggctact acaacttcga cggcaatgtc cttccacacc ctcctatcct
     3601 gcgcggcgtg gaaactaccg tcgccgcact cgccaaagcc ggtcacaccg tgaccccgtg
     3661 gacgccatac aagcacgatt tcggccacga tctcatctcc catatctacg cggctgacgg
     3721 cagcgccgac gtaatgcgcg acatcagtgc atccggcgag ccggcgattc caaatatcaa
     3781 agacctactg aacccgaaca tcaaagctgt taacatgaac gagctctggg acacgcatct
     3841 ccagaagtgg aattaccaga tggagtacct tgagaaatgg cgggaggctg aagaaaaggc
     3901 cgggaaggaa ctggacgcca tcatcgcgcc gattacgcct accgctgcgg tacggcatga
     3961 ccagttccgg tactatgggt atgcctctgt gatcaacctg ctggatttca cgagcgtggt
     4021 tgttccggtt acctttgcgg ataagaacat cgataagaag aatgagagtt tcaaggcggt
     4081 tagtgagctt gatgccctcg tgcaggaaga gtatgatccg gaggcgtatc atggggcacc
     4141 ggttgcagtg caggttatcg gacggagact cagtgaagag aggacgttgg cgattgcaga
     4201 ggaagtgggg aagttgctgg gaaatgtggt gactccatag cccggggggg gctcgatccc
     4261 ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt ctaccggcag
     4321 tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca tgcccccgaa
     4381 ctgcaggagt ggggaggcac gatggccgct ttggtcgatc tagattacgt ggaagaaagg
     4441 tagtaaaagt agtagtataa gtagtaaaaa gaggtaaaaa gagaaaaccg gctacatact
     4501 agagaagcac gtacacaaaa actcataggc acttcatcat acgacagttt cttgatgcat
     4561 tataatagtg tattagatat tttcagaaat atgcatagaa cctcctcttg cctttacttt
     4621 ttatacatag aacattggca gatttactta cactactttg tttctacgcc atttcttttg
     4681 ttttcaacac ttagacaagt tgttgagaac cggactacta aaaagcaatg ttcccactga
     4741 aaatcatgta cctgcagcat aataaccccc taattctgca tcgatccagt atgttttttt
     4801 ttctctactc atttttacct gaagatagag cttctaaaac aaaaaaaatc agtgattaca
     4861 tgcatattgt gtgttctagt aaccaaagga aaggaacaga tagataaaat tccgagactg
     4921 tcaaattagg tttttttctt tttttttggc gggagtcagt gggccgaaat atgttcttgg
     4981 cctagaactt aatctggttt gatcatgcca atacttgcct gagtgcccga ctttttgccc
     5041 accctcttgc cttctgtcat ccttcaaaac ccacctgttt tccagccgta tcttcgctcg
     5101 catctacaca tactgtgcca tatcttgtgt gtagccggac gtgactatga ccaaaaacaa
     5161 acaaggagaa ctgttcgccg atttgtaaca ctcctgcatc catccaagtg ggtatgcgct
     5221 atgcaatgtt aagctaggtc aggtcagacc aggtccaagg acagcaactt gactgtatgc
     5281 aacctttacc atctttgcac agaacatact tgtagctagc tagttacact tatggaccga
     5341 aaaggcaccc caccatgtct gtccggcttt agagtacggc cgcagaccgc tgatttgcct
     5401 tgccaagcag tagtcacaat gcatcgcatg agcacacggg cacgggcacg ggcacaggaa
     5461 ccattggcaa aaataccaga tacactatac cgacgtatat caagcccaag tttaaaattc
     5521 ctaaatttcc gcggctactt ttcaattccc tatagtgagt cgtattaaat tcgtaatcat
     5581 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5641 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5701 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5761 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5821 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5881 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5941 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6001 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6061 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6121 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6181 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6241 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6301 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6361 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6421 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6481 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6541 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6601 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6661 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6721 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6781 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6841 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6901 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6961 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7021 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7081 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7141 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7201 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7261 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7321 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7381 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7441 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7501 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7561 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7621 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7681 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     7741 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7801 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7861 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     7921 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7981 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8041 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8101 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8161 ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg
     8221 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     8281 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     8341 gccagtgcca agctcccgcg gggatcgact cataaaatag taaccttcta atgcgtatct
     8401 attgactacc aaccattagt gtggttgcag aaggcggaat tttcccttct tcgaatttag
     8461 cttgcttttt cattttttat tttccatttt tcagtttttg tttgtgtcga atttagccag
     8521 ttgcttctcc aagatgaaaa aaacccctgc gcagtttctg tgctgcaaga tcctaatcga
     8581 cttttccacc ccccacaaaa gtaaatgttc ttttgttaca ttcgcgtggg tagctagctc
     8641 cccgaatctt caaaggactt agggactgca ctacatcaga gtgtgttcac ctggtttgct
     8701 gcctggtttg aaagaaaaga gcagggaact cgcgggttcc cggcgaataa tcatgcgata
     8761 gtcctttggc cttccaagtc gcatgtagag tagacaacag acagggaggg caggaaggat
     8821 ctttcactga gatcctgtat cttgttgggt aagtcggatg aaaggggaat cgtatgagat
     8881 tggagaggat gcggaagagg taacgccttt tgttaacttg tttaattatt atggggcagg
     8941 cgagaggggg aggaatgtat gtgtgtgagg cgggcgagac ggagccatcc aggccaggta
     9001 gaaatagaga aagccgaatg ttagacaata tggcagcgta gtagagtagg taggtaggca
     9061 agtactgcta gcaaagagga gaagggtaag ctcactcttc gcattccaca ccgttagtgt
     9121 gtcagtttga acaaaaaaac aatcatcata ccaattgatg gactgtggac tggcttttgg
     9181 aacggctttt cggactgcga ttattcgtga ggaatcaagg taggaatttg gtcatattta
     9241 cggacaacag tgggtgattc ccatatcgag taggaaaacg agatcatggt atcctcagat
     9301 atgttgcgga aatctgttca ccgcaaagtt cagggtgctc tggtgggttt cggttggtct
     9361 ttgctttgct tctcccttgt cttgcatgtt aataatagcc tagcctgtga gccgaaactt
     9421 agggtaggct tagtgttgga acgtacatat ctatcacgtt gacttggttt aaccaggcga
     9481 cctggtagcc agccataccc acacacgttt tttgtatctt cagtatagtt gtgaaaagtg
     9541 tagcggaaat ttgtggtccg agcaacagcg tctttttcta gtagtgcggt cggttacttg
     9601 gttgacattg gtatttggac tttgttgcta caccattcac tacttgaagt cgagtgtgaa
     9661 gggtatgatt tctagtggtg aacaccttta gttacgtaat gttttcattg ctgttttact
     9721 tgagatttcg attgagaaaa aggtatttaa tagctcgaat caatgtgaga acagagagaa
     9781 gatgttcttc cctaactcga aaggtatatg aggcttgtgt ttcttaggag aattattatt
     9841 cttttgttat gttgcgcttg tagttggaaa aggtgaagag acaaaagctg gaattgtgag
     9901 cggataacaa gctcaacact tgaaatttag gaaagagcag aatttggcaa aaaaaataaa
     9961 aaaaaaataa acacacatac tcatcgag
//
