LOCUS       40924_26879        9991 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pKLMF-EK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Ganatra MB, Read JD, Colussi PA, Taron CH.
  TITLE     Vectors for intracellular expression of maltose binding protein 
            fusions in the yeast Kluyveromyces lactis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   Ganatra MB, Read JD, Colussi PA, Taron CH.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2008) Gene Expression Division, New England 
            Biolabs, 240 County Road, Ipswich, MA 01938, USA
REFERENCE   3  (bases 1 to 9991)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9991)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-AUG-2008) Gene Expression Division, New England Biolabs, 240 
            County Road, Ipswich, MA 01938, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..1113
                     /codon_start=1
                     /label=MBP
                     /note="maltose binding protein from E. coli"
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             1126..1140
                     /codon_start=1
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
                     /translation="DDDDK"
     misc_feature    1141..1237
                     /label=multiple cloning site
                     /note="multiple cloning site"
     terminator      1255..1873
                     /label=LAC4 terminator
                     /note="transcription terminator for Kluyveromyces lactis
                     LAC4"
     promoter        1874..2576
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             2597..4240
                     /codon_start=1
                     /label=amdS
                     /note="acetamidase from Aspergillus nidulans"
                     /translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGIL
                     SEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALAQA
                     RELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRK
                     AGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVG
                     TDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFT
                     KSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPPILRGVE
                     TTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAIPNIKDLL
                     NPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPTAAVRHDQF
                     RYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYDPEAYHGAPV
                     AVQVIGRRLSEERTLAIAEEVGKLLGNVVTP"
     promoter        4879..5535
                     /label=LAC4 promoter (5')
                     /note="5' portion of the Kluyveromyces lactis LAC4
                     promoter"
     promoter        complement(5552..5570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5584..5600)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5608..5624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5632..5662)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5677..5698)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5986..6574)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6748..7605)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7606..7710)
                     /label=AmpR promoter
     rep_origin      7737..8192
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8333..8349
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8363..9891
                     /label=LAC4 promoter (3')
                     /note="3' portion of a modified Kluyveromyces lactis LAC4 
                     promoter"
     protein_bind    9897..9913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aagcttgcca ccatgaaaac tgaagaaggt aaactggtaa tctggattaa cggcgataaa
       61 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
      121 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
      181 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
      241 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
      301 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg
      361 atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg
      421 gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac
      481 ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag
      541 tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg
      601 gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct
      661 gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc
      721 gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc
      781 aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg
      841 gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa
      901 gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca
      961 cgtattgccg ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag
     1021 atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag
     1081 actgtcgatg aagccctgaa agacgcgcag actaattcga gctcggatga cgatgacaag
     1141 ctcgagaaaa gagaggctga agctagaaga gctcatatgt ccatgggcgg ccgcgatatc
     1201 gtcgacggat ccgaattccc tgcaggtaat taaataaagg ccttgaatcg agaatttata
     1261 cttagataag tatgtactta caggtatatt tctatgagat actgatgtat acatgcatga
     1321 taatatttaa acggttatta gtgccgattg tcttgtgcga taatgacgtt cctatcaaag
     1381 caatacactt accacctatt acatgggcca agaaaatatt ttcgaacttg tttagaatat
     1441 tagcacagag tatatgatgt tatccgttag attatgcatg attcattcct acaacttttt
     1501 cgtagcataa ggattaatta cttggatgcc aataaaaaaa aaaaacatcg agaaaatttc
     1561 agcatgctca gaaacaattg cagtgtatca aagtaaaaaa aagattttca ctacatgttc
     1621 cttttgaaga aagaaaatca tggaacatta gatttacaaa aatttaacca ccgctgatta
     1681 acgattagac cgttaagcgc acaacaggtt attagtacag agaaagcatt ctgtggtgtt
     1741 gccccggact ttcttttgcg acataggtaa atcgaatacc atcatactat cttttccaat
     1801 gactccctaa agaaagactc ttcttcgatg ttgtatacgt tggagcatag ggcaagaatt
     1861 gtggcttgag atcatccttt tgttgtttcc gggtgtacaa tatggacttc ctcttttctg
     1921 gcaaccaaac ccatacatcg ggattcctat aataccttcg ttggtctccc taacatgtag
     1981 gtggcggagg ggagatatac aatagaacag ataccagaca agacataatg ggctaaacaa
     2041 gactacacca attacactgc ctcattgatg gtggtacata acgaactaat actgtagccc
     2101 tagacttgat agccatcatc atatcgaagt ttcactaccc tttttccatt tgccatctat
     2161 tgaagtaata ataggcgcat gcaacttctt ttcttttttt ttcttttctc tctcccccgt
     2221 tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt
     2281 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa
     2341 gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat
     2401 acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca
     2461 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt
     2521 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaagcaa
     2581 ttccagatct gccaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
     2641 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg aagacagcgt
     2701 tattgatttc ccaaagaaat cgggcatcct ttcagaggcc gaactgaaga tcacagaggc
     2761 ttccgctgcg gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
     2821 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta acaaactgcg cccacgagtt
     2881 cttccctgac gccgctctcg cgcaggcaag ggaactcgat gaatactacg caaagcacaa
     2941 gagacccgtt ggtccacttc atggcctccc catctctctc aaagaccagc ttcgagtcaa
     3001 gggctacgaa acatcaatgg gctacatctc atggctaaac aagtacgacg aaggggactc
     3061 ggttctgaca accatgctcc gcaaagccgg tgccgtcttc tacgtcaaga cctctgtccc
     3121 gcagaccctg atggtctgcg agacagtcaa caacatcatc gggcgcaccg tcaacccacg
     3181 caacaagaac tggtcgtgcg gcggcagttc tggtggtgag ggtgcgatcg ttgggattcg
     3241 tggtggcgtc atcggtgtag gaacggacat cggtggctcg attcgagtgc cggccgcgtt
     3301 caacttcctg tacggtctaa ggccgagtca tgggcggctg ccgtatgcaa agatggcgaa
     3361 cagcatggag ggtcaggaga cggtgcacag cgttgtcggg ccgattacgc actctgttga
     3421 ggacctccgc ctcttcacca aatccgtcct cggtcaggag ccttggaaat acgactccaa
     3481 ggtcatcccc atgccctggc gccagtccga gtcggacatt attgcctcca agatcaagaa
     3541 cggcgggctc aatatcggct actacaactt cgacggcaat gtccttccac accctcctat
     3601 cctgcgcggc gtggaaacta ccgtcgccgc actcgccaaa gccggtcaca ccgtgacccc
     3661 gtggacgcca tacaagcacg atttcggcca cgatctcatc tcccatatct acgcggctga
     3721 cggcagcgcc gacgtaatgc gcgacatcag tgcatccggc gagccggcga ttccaaatat
     3781 caaagaccta ctgaacccga acatcaaagc tgttaacatg aacgagctct gggacacgca
     3841 tctccagaag tggaattacc agatggagta ccttgagaaa tggcgggagg ctgaagaaaa
     3901 ggccgggaag gaactggacg ccatcatcgc gccgattacg cctaccgctg cggtacggca
     3961 tgaccagttc cggtactatg ggtatgcctc tgtgatcaac ctgctggatt tcacgagcgt
     4021 ggttgttccg gttacctttg cggataagaa catcgataag aagaatgaga gtttcaaggc
     4081 ggttagtgag cttgatgccc tcgtgcagga agagtatgat ccggaggcgt atcatggggc
     4141 accggttgca gtgcaggtta tcggacggag actcagtgaa gagaggacgt tggcgattgc
     4201 agaggaagtg gggaagttgc tgggaaatgt ggtgactcca tagcccgggg ggggctcgat
     4261 cccctcgcga gttggttcag ctgctgcctg aggctggacg acctcgcgga gttctaccgg
     4321 cagtgcaaat ccgtcggcat ccaggaaacc agcagcggct atccgcgcat ccatgccccc
     4381 gaactgcagg agtggggagg cacgatggcc gctttggtcg atctagatta cgtggaagaa
     4441 aggtagtaaa agtagtagta taagtagtaa aaagaggtaa aaagagaaaa ccggctacat
     4501 actagagaag cacgtacaca aaaactcata ggcacttcat catacgacag tttcttgatg
     4561 cattataata gtgtattaga tattttcaga aatatgcata gaacctcctc ttgcctttac
     4621 tttttataca tagaacattg gcagatttac ttacactact ttgtttctac gccatttctt
     4681 ttgttttcaa cacttagaca agttgttgag aaccggacta ctaaaaagca atgttcccac
     4741 tgaaaatcat gtacctgcag cataataacc ccctaattct gcatcgatcc agtatgtttt
     4801 tttttctcta ctcattttta cctgaagata gagcttctaa aacaaaaaaa atcagtgatt
     4861 acatgcatat tgtgtgttct agtaaccaaa ggaaaggaac agatagataa aattccgaga
     4921 ctgtcaaatt aggttttttt cttttttttt ggcgggagtc agtgggccga aatatgttct
     4981 tggcctagaa cttaatctgg tttgatcatg ccaatacttg cctgagtgcc cgactttttg
     5041 cccaccctct tgccttctgt catccttcaa aacccacctg ttttccagcc gtatcttcgc
     5101 tcgcatctac acatactgtg ccatatcttg tgtgtagccg gacgtgacta tgaccaaaaa
     5161 caaacaagga gaactgttcg ccgatttgta acactcctgc atccatccaa gtgggtatgc
     5221 gctatgcaat gttaagctag gtcaggtcag accaggtcca aggacagcaa cttgactgta
     5281 tgcaaccttt accatctttg cacagaacat acttgtagct agctagttac acttatggac
     5341 cgaaaaggca ccccaccatg tctgtccggc tttagagtac ggccgcagac cgctgatttg
     5401 ccttgccaag cagtagtcac aatgcatcgc atgagcacac gggcacgggc acgggcacag
     5461 gaaccattgg caaaaatacc agatacacta taccgacgta tatcaagccc aagtttaaaa
     5521 ttcctaaatt tccgcggcta cttttcaatt ccctatagtg agtcgtatta aattcgtaat
     5581 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5641 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5701 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5761 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5821 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5881 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5941 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6001 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6061 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6121 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6181 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6241 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6301 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6361 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6421 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6481 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6541 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6601 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6661 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6721 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6781 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6841 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6901 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6961 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7021 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7081 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7141 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7201 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7261 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7321 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7381 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7441 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7501 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7561 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7621 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7681 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     7741 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7801 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7861 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7921 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7981 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8041 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8101 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8161 gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt
     8221 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8281 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8341 acggccagtg ccaagctccc gcggggatcg actcataaaa tagtaacctt ctaatgcgta
     8401 tctattgact accaaccatt agtgtggttg cagaaggcgg aattttccct tcttcgaatt
     8461 tagcttgctt tttcattttt tattttccat ttttcagttt ttgtttgtgt cgaatttagc
     8521 cagttgcttc tccaagatga aaaaaacccc tgcgcagttt ctgtgctgca agatcctaat
     8581 cgacttttcc accccccaca aaagtaaatg ttcttttgtt acattcgcgt gggtagctag
     8641 ctccccgaat cttcaaagga cttagggact gcactacatc agagtgtgtt cacctggttt
     8701 gctgcctggt ttgaaagaaa agagcaggga actcgcgggt tcccggcgaa taatcatgcg
     8761 atagtccttt ggccttccaa gtcgcatgta gagtagacaa cagacaggga gggcaggaag
     8821 gatctttcac tgagatcctg tatcttgttg ggtaagtcgg atgaaagggg aatcgtatga
     8881 gattggagag gatgcggaag aggtaacgcc ttttgttaac ttgtttaatt attatggggc
     8941 aggcgagagg gggaggaatg tatgtgtgtg aggcgggcga gacggagcca tccaggccag
     9001 gtagaaatag agaaagccga atgttagaca atatggcagc gtagtagagt aggtaggtag
     9061 gcaagtactg ctagcaaaga ggagaagggt aagctcactc ttcgcattcc acaccgttag
     9121 tgtgtcagtt tgaacaaaaa aacaatcatc ataccaattg atggactgtg gactggcttt
     9181 tggaacggct tttcggactg cgattattcg tgaggaatca aggtaggaat ttggtcatat
     9241 ttacggacaa cagtgggtga ttcccatatc gagtaggaaa acgagatcat ggtatcctca
     9301 gatatgttgc ggaaatctgt tcaccgcaaa gttcagggtg ctctggtggg tttcggttgg
     9361 tctttgcttt gcttctccct tgtcttgcat gttaataata gcctagcctg tgagccgaaa
     9421 cttagggtag gcttagtgtt ggaacgtaca tatctatcac gttgacttgg tttaaccagg
     9481 cgacctggta gccagccata cccacacacg ttttttgtat cttcagtata gttgtgaaaa
     9541 gtgtagcgga aatttgtggt ccgagcaaca gcgtcttttt ctagtagtgc ggtcggttac
     9601 ttggttgaca ttggtatttg gactttgttg ctacaccatt cactacttga agtcgagtgt
     9661 gaagggtatg atttctagtg gtgaacacct ttagttacgt aatgttttca ttgctgtttt
     9721 acttgagatt tcgattgaga aaaaggtatt taatagctcg aatcaatgtg agaacagaga
     9781 gaagatgttc ttccctaact cgaaaggtat atgaggcttg tgtttcttag gagaattatt
     9841 attcttttgt tatgttgcgc ttgtagttgg aaaaggtgaa gagacaaaag ctggaattgt
     9901 gagcggataa caagctcaac acttgaaatt taggaaagag cagaatttgg caaaaaaaat
     9961 aaaaaaaaaa taaacacaca tactcatcga g
//
