LOCUS       40924_26839       14206 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKL13, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14206)
  AUTHORS   Lam KN.
  TITLE     Developing a Bacteroides system for function-based screening of DNA 
            from the human gut microbiome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14206)
  AUTHORS   Lam KN.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2016) Biology, University of Waterloo, 200 
            University Avenue West, Waterloo, Canada
REFERENCE   3  (bases 1 to 14206)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14206)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2016) Biology, University of Waterloo, 200 University Avenue
            West, Waterloo, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     288..304
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        311..329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    332..337
                     /label=EcoRI site
                     /note="EcoRI site"
     CDS             complement(931..1620)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein"
                     /label=repA
                     /protein_id="AOP12487.1"
                     /translation="MILNRKIIFPLILFLWKIIVYFVENNISQKHIKMENKKAVKLTDF
                     QKNEENPFMKQAIEGIENHVVKKYKSNSGGDKRAVVALADTETGEVFKTSFIRQIEVDE
                     EQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMKPKNDLIIFNRKKCLEYTKYKTDKAVY
                     KGLAELVKAEIIARGPADNLWFINPLIVFNGDRVTFAKTYVRKKTLAAQKKEEAEKRQL
                     SLGFDEQ"
     gene            complement(931..1620)
                     /gene="repA"
                     /label=repA
     CDS             3183..3983
                     /codon_start=1
                     /gene="ermF"
                     /product="erythromycin resistance"
                     /label=ermF
                     /protein_id="AOP12484.1"
                     /translation="MTKKKLPVRFTGQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKG
                     FLTVHLLKIANNVVAIENDTALVEHLRKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNI
                     PYGITSDIFKILMFESLGNFLGGSIVLQLEPTQKLFSRKLYNPYTVFYHTFFDLKLVYE
                     VGPESFLPPPTVKSALLNIKRKHLFFDFKFKAKYLAFISCLLEKPDLSVKTALKSIFRK
                     SQVRSISEKFGLNLNAQIVCLSPSQWLNCFLEMLEVVPEKFHPS"
     gene            3183..3983
                     /gene="ermF"
                     /label=ermF
     misc_feature    4133..4138
                     /label=EcoRI site
                     /note="EcoRI site"
     misc_feature    4167..4174
                     /label=SgsI site
                     /note="SgsI site"
     misc_feature    4183..4190
                     /label=PacI site
                     /note="PacI site"
     regulatory      complement(4191..4279)
                     /label=ilvGEDA transcriptional terminator
                     /note="ilvGEDA transcriptional terminator"
                     /regulatory_class="terminator"
     primer_bind     4280..4310
                     /label=KL-JC103 sequencing primer
                     /note="KL-JC103 sequencing primer"
     misc_feature    complement(4311..4321)
                     /label=3-frame translational stop 2
                     /note="3-frame translational stop 2"
     misc_feature    4322..4327
                     /label=NheI site
                     /note="NheI site"
     misc_feature    4328..4333
                     /label=Eco72I site
                     /note="Eco72I site"
     misc_feature    4334..4340
                     /label=Eco81I site
                     /note="Eco81I site"
     protein_bind    4349..4365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4373..4401)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     misc_feature    4408..4414
                     /label=Eco81I site
                     /note="Eco81I site"
     misc_feature    4415..4420
                     /label=BstBI site
                     /note="BstBI site"
     CDS             4607..5407
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     misc_feature    5476..5481
                     /label=BstBI site
                     /note="BstBI site"
     misc_feature    5482..5487
                     /label=Eco72I site
                     /note="Eco72I site"
     misc_feature    5488..5493
                     /label=NsiI site
                     /note="NsiI site"
     misc_feature    5494..5504
                     /label=3-frame translational stop 1
                     /note="3-frame translational stop 1"
     primer_bind     complement(5505..5535)
                     /label=KL-JC102 sequencing primer
                     /note="KL-JC102 sequencing primer"
     primer_bind     complement(5506..5522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      5536..5617
                     /label=rnpB T1 transcriptional terminator
                     /note="rnpB T1 transcriptional terminator"
                     /regulatory_class="terminator"
     misc_feature    5618..5624
                     /label=CpoI site
                     /note="CpoI site"
     misc_feature    5633..5640
                     /label=SfaAI site
                     /note="SfaAI site"
     oriT            5822..5931
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             5964..6332
                     /label=traJ
                     /note="oriT-recognizing protein"
     primer_bind     complement(6510..6526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6534..6550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6558..6588)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6603..6624)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(6847..7503)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7504..7606)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             7722..8069
                     /codon_start=1
                     /gene="redF"
                     /product="RedF"
                     /label=redF
                     /protein_id="AOP12489.1"
                     /translation="MERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVP
                     VTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPE
                     SDAVAMLKQLS"
     gene            7722..8069
                     /gene="redF"
                     /label=redF
     rep_origin      8464..9078
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      9154..9373
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             9464..10216
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    10222..10472
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             10798..11970
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             11973..12941
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    13017..13490
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(13750..14148)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(14166..14199)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 cagtgaattg taatacgact cactataggg cgaattcata acagccggtg acagccggct
      361 gacagggggt taagggggct tgtcccctta cacacgcact ctttagggtg ctagtgtgct
      421 atcaccatac tgcataggtg cgaagttagt gaatgttttg taaatgcaca aataaaggga
      481 aaaacatttg gatttgcgat aataaagtac tacctttgtt gctgaccaaa cggtagctga
      541 ccgatacggg agagttacca aaatacaagc cgctggagtt aattgacgga catccgacat
      601 ctccagcggc tttatttttg cctatctgct tcgcctaggc acaccagtac ctctactaaa
      661 aatgtacttc aaagatactt attttctacc gacttgatag tttttacccc atattcttgg
      721 acatttttcc cccatgaggt tatctttgta gggtgaaaga gaaacccata aacggggata
      781 gattgaatgc tgggaagcat aaacaatcgg ggtaaggtta gcgaaccttg cctttcatcc
      841 cccattataa ctttacatag aggaacttta tctatccccc cccgccccca aagggggagc
      901 gaccaaacgg cagcttcact caatggagtg ttactgttca tcaaagccaa gtgataattg
      961 tcgtttctct gcttcttctt tcttttgggc agctaaagtc tttttccgaa cgtatgtttt
     1021 agcaaatgtc actcggtcac cattgaatac tatcagagga ttaataaacc aaagattatc
     1081 ggctggtcct cgggctatga tttcagcttt tacaagttct gcaagtcctt tataaacggc
     1141 tttgtctgtt ttgtatttgg tatattctag gcattttttt ctattgaaaa tgattaaatc
     1201 atttttgggt ttcatgcagg tcataaagta accaaaaacc cgaatagctg cttgtgatag
     1261 gtcaaagaat gcagcaaagt tagaaagata caatttagtg aattgttctt catctacttc
     1321 tatttgacgg ataaacgaag tcttaaacac ttctccagtt tcagtgtcgg ctaaagctac
     1381 tacagctctc ttatcgccac cactattact cttatacttt ttaacaacat gattttcaat
     1441 accttctata gcttgtttca taaaaggatt ttcttcgttc ttttgaaaat cggttaactt
     1501 aactgctttt ttattttcca ttttgatatg tttttgggaa atattattct ccacaaagta
     1561 aactattatt ttccataaaa acaatattaa gggaaatatt attttcctat ttagtatcat
     1621 attaggaaat cggtattttc tagattggaa aatgagaatt tccaatatgg aaaatgccct
     1681 atattgtgta tcaagtactt aacttattct atttctttta ttcttaatat acccccaaaa
     1741 cagcacaaaa tcagtcactt aaaaatcatc ggtcggggaa tggtgcactc tcagtacaat
     1801 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     1861 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     1921 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     1981 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaaa ttctgattaa
     2041 taatttgttt aaatttttcg tttggcgtga ggtatccaag tcttttacga ggtcgattat
     2101 tgagtttatt ttcaatccac ttaatctgtt tgttggttac ttcactaaag tccttaccct
     2161 ttgggatata ctgcctgata agcccgttgg tgttttcatt ggcaccacgt tcccatgagt
     2221 ggtatggttt gcaaaaatag aattttattt ccaatttttg cgcaatttcc tcgtgctttg
     2281 caaactcctt tccattgtca gccgtaattg tgtgtattaa gtttttcact ttccgcagtg
     2341 cccatactgc aatcttagct accgggatgg cttcttttcc cgacaacttg cgtatccaga
     2401 ccctgcttgt tgctctgtcg ttaatggtaa gaatggcacc tttgtggttc ttaccaataa
     2461 ttgtatctat ctctaaatca ccaaatctct ccttcagttc cactatctcg ggacgctcat
     2521 caatatccac cctgcctggg ataaatcctc gccctgcatt tttagaacca cgtttggcat
     2581 acctgcgacc ttgtctgcga agatatttgt gcagtttgcc accccgccgc ttatcctccc
     2641 aaatccagcg atatatcgtt tcgtgagata ccatcgcaat tccctccaag cggctcctgc
     2701 cgacaatctg ctccgggctg aatcctttct tcaacagctt tattatccgt tttctcattg
     2761 ccggtgtaag cacttccttg cgatgttttt gctgcttgcg cctgtctgct tttcgctggg
     2821 caagctccat gctatagcta ccacttcggg cgtcgcaatt gcgctttatc tccctgtaaa
     2881 cagtgctttt atctactccg atagcttccg ctattgcttt tttgctcatc ggtatttgca
     2941 acatcataga aattgcatac ctttgttcct cggttatatg tttgctcatc tgcaactttt
     3001 ttttctttgg acggacaatt aaagcaaaga tagcaaactt tatccattca gagtgagaga
     3061 aagggggaca ttgtctctct ttcctctctg aaaaataaat gtttttattg cttattatcc
     3121 gcacccaaaa agttgcattt ataagttgaa ctcaagaagt attcacctgt aagaagttac
     3181 taatgacaaa aaagaaattg cccgttcgtt ttacgggtca gcactttact attgataaag
     3241 tgctaataaa agatgcaata agacaagcaa atataagtaa tcaggatacg gttttagata
     3301 ttggggcagg caaggggttt cttactgttc atttattaaa aatcgccaac aatgttgttg
     3361 ctattgaaaa cgacacagct ttggttgaac atttacgaaa attattttct gatgcccgaa
     3421 atgttcaagt tgtcggttgt gattttagga attttgcagt tccgaaattt cctttcaaag
     3481 tggtgtcaaa tattccttat ggcattactt ccgatatttt caaaatcctg atgtttgaga
     3541 gtcttggaaa ttttctggga ggttccattg tccttcaatt agaacctaca caaaagttat
     3601 tttcgaggaa gctttacaat ccatataccg ttttctatca tacttttttt gatttgaaac
     3661 ttgtctatga ggtaggtcct gaaagtttct tgccaccgcc aactgtcaaa tcagccctgt
     3721 taaacattaa aagaaaacac ttattttttg attttaagtt taaagccaaa tacttagcat
     3781 ttatttcctg tctgttagag aaacctgatt tatctgtaaa aacagcttta aagtcgattt
     3841 tcaggaaaag tcaggtcagg tcaatttcgg aaaaattcgg tttaaacctt aatgctcaaa
     3901 ttgtttgttt gtctccaagt caatggttaa actgtttttt ggaaatgctg gaagttgtcc
     3961 ctgaaaaatt tcatccttcg tagttcaaag tcgggtggtt gtcaagatga tttttttggt
     4021 ttggtgtcgt ctttttttaa gctgccgcat aacggctggc aaattggcga tggagccgac
     4081 ttttagcaca aatgttgaat agaattacta atcttcaaca ttgcacaaaa gtgaattcga
     4141 gctcggtacc cggggatccc acaaatggcg cgccggctgg atttaattaa tgtctgctcc
     4201 tcggttatgt ttttaaggtc aaaaaaaacc cccggacctt tcggtgcggg ggtcttagtt
     4261 cgttaaggct tgatctctag cgattaagtt gggtaacgcc agggttttcg tcacttagtc
     4321 agctagccac gtgccttagg gtgtgaaatt gttatccgct cacaattcca cacattatac
     4381 gagccgatga ttaattgtca acagctccct gaggttcgaa gatcctccgg ctcacggtaa
     4441 ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc
     4501 cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata agtttatcac
     4561 caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt catcagcggt
     4621 ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc agcttctcaa
     4681 ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta gcgtccggcc
     4741 cctcgaagat gggccacttg gactgatcga ggccctgcgt gctgcgctgg gtccgggagg
     4801 gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc ctgccacgtc
     4861 gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc caaatgtaaa
     4921 gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc agatcatctc
     4981 tgatccattg cccctgccac ctcactcgcc tgcaagcccg gtcgcccgtg tccatgaact
     5041 cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc tgcatcttgc
     5101 cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc ttcaggatgg
     5161 caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct ttgccttggc
     5221 ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc atgcctttgc
     5281 tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg gccgagatcc
     5341 gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg ctcgccagtc
     5401 gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg tcgcgctgat
     5461 tgctggggca acacgttcga acacgtgatg cattaactag gtgacgtcat agctgtttcc
     5521 tgtgtgaaat tgttatcggt cagtttcacc tgatttacgt aaaaacccgc ttcggcgggt
     5581 ttttgctttt ggaggggcag aaagatgaat gactgtccgg tccgagcagg tcgcgatcgc
     5641 atttgtggga tcctctagag tcgacctgca ggcatgcaag cttgcgcttt tccgctgcat
     5701 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     5761 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     5821 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     5881 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg
     5941 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc
     6001 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg
     6061 cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg
     6121 gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc
     6181 tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg
     6241 ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca
     6301 tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg
     6361 ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg
     6421 gagctggtga agtacatcac cgacgagcaa ggcaagaccg aaagcttgag tattctatag
     6481 tctcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6541 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6601 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6661 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg aaccccttgc ggccgcccgg
     6721 gccgtcgacc aattctcatg tttgacagct tatcatcgaa tttctgccat tcatccgctt
     6781 attatcactt attcaggcgt agcaaccagg cgtttaaggg caccaataac tgccttaaaa
     6841 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     6901 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     6961 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     7021 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     7081 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     7141 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     7201 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     7261 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     7321 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     7381 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     7441 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     7501 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     7561 tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg
     7621 ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc
     7681 gaagtgatct tccgtcacag gtatttattc gcgataagct catggagcgg cgtaaccgtc
     7741 gcacaggaag gacagagaaa gcgcggatct gggaagtgac ggacagaacg gtcaggacct
     7801 ggattgggga ggcggttgcc gccgctgctg ctgacggtgt gacgttctct gttccggtca
     7861 caccacatac gttccgccat tcctatgcga tgcacatgct gtatgccggt ataccgctga
     7921 aagttctgca aagcctgatg ggacataagt ccatcagttc aacggaagtc tacacgaagg
     7981 tttttgcgct ggatgtggct gcccggcacc gggtgcagtt tgcgatgccg gagtctgatg
     8041 cggttgcgat gctgaaacaa ttatcctgag aataaatgcc ttggccttta tatggaaatg
     8101 tggaactgag tggatatgct gtttttgtct gttaaacaga gaagctggct gttatccact
     8161 gagaagcgaa cgaaacagtc gggaaaatct cccattatcg tagagatccg cattattaat
     8221 ctcaggagcc tgtgtagcgt ttataggaag tagtgttctg tcatgatgcc tgcaagcggt
     8281 aacgaaaacg atttgaatat gccttcagga acaatagaaa tcttcgtgcg gtgttacgtt
     8341 gaagtggagc ggattatgtc agcaatggac agaacaacct aatgaacaca gaaccatgat
     8401 gtggtctgtc cttttacagc cagtagtgct cgccgcagtc gagcgacagg gcgaagccct
     8461 cggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa
     8521 acgccgtcga agccgtgtgc gagacaccgc ggccggccgc cggcgttgtg gatacctcgc
     8581 ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag gggccgactc
     8641 acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga cgtggagctg
     8701 gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac agatgatgtg
     8761 gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga ctactgacag
     8821 atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag gggcgcacct
     8881 attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg gtttccgccc
     8941 gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat ttataaacct
     9001 tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc
     9061 cccttctcga accctcccgg tcgagtgagc gaggaagcac cagggaacag cacttatata
     9121 ttctgcttac acacgatgcc tgaaaaaact tcccttgggg ttatccactt atccacgggg
     9181 atatttttat aattattttt tttatagttt ttagatcttc ttttttagag cgccttgtag
     9241 gcctttatcc atgctggttc tagagaaggt gttgtgacaa attgcccttt cagtgtgaca
     9301 aatcaccctc aaatgacagt cctgtctgtg acaaattgcc cttaaccctg tgacaaattg
     9361 ccctcagaag aagctgtttt ttcacaaagt tatccctgct tattgactct tttttattta
     9421 gtgtgacaat ctaaaaactt gtcacacttc acatggatct gtcatggcgg aaacagcggt
     9481 tatcaatcac aagaaacgta aaaatagccc gcgaatcgtc cagtcaaacg acctcactga
     9541 ggcggcatat agtctctccc gggatcaaaa acgtatgctg tatctgttcg ttgaccagat
     9601 cagaaaatct gatggcaccc tacaggaaca tgacggtatc tgcgagatcc atgttgctaa
     9661 atatgctgaa atattcggat tgacctctgc ggaagccagt aaggatatac ggcaggcatt
     9721 gaagagtttc gcggggaagg aagtggtttt ttatcgccct gaagaggatg ccggcgatga
     9781 aaaaggctat gaatcttttc cttggtttat caaacgtgcg cacagtccat ccagagggct
     9841 ttacagtgta catatcaacc catatctcat tcccttcttt atcgggttac agaaccggtt
     9901 tacgcagttt cggcttagtg aaacaaaaga aatcaccaat ccgtatgcca tgcgtttata
     9961 cgaatccctg tgtcagtatc gtaagccgga tggctcaggc atcgtctctc tgaaaatcga
    10021 ctggatcata gagcgttacc agctgcctca aagttaccag cgtatgcctg acttccgccg
    10081 ccgcttcctg caggtctgtg ttaatgagat caacagcaga actccaatgc gcctctcata
    10141 cattgagaaa aagaaaggcc gccagacgac tcatatcgta ttttccttcc gcgatatcac
    10201 ttccatgacg acaggatagt ctgagggtta tctgtcacag atttgagggt ggttcgtcac
    10261 atttgttctg acctactgag ggtaatttgt cacagttttg ctgtttcctt cagcctgcat
    10321 ggattttctc atactttttg aactgtaatt tttaaggaag ccaaatttga gggcagtttg
    10381 tcacagttga tttccttctc tttcccttcg tcatgtgacc tgatatcggg ggttagttcg
    10441 tcatcattga tgagggttga ttatcacagt ttattactct gaattggcta tccgcgtgtg
    10501 tacctctacc tggagttttt cccacggtgg atatttcttc ttgcgctgag cgtaagagct
    10561 atctgacaga acagttcttc tttgcttcct cgccagttcg ctcgctatgc tcggttacac
    10621 ggctgcggcg agcgctagtg ataataagtg actgaggtat gtgctcttct tatctccttt
    10681 tgtagtgttg ctcttatttt aaacaacttt gcggtttttt gatgactttg cgattttgtt
    10741 gttgctttgc agtaaattgc aagatttaat aaaaaaacgc aaagcaatga ttaaaggatg
    10801 ttcagaatga aactcatgga aacacttaac cagtgcataa acgctggtca tgaaatgacg
    10861 aaggctatcg ccattgcaca gtttaatgat gacagcccgg aagcgaggaa aataacccgg
    10921 cgctggagaa taggtgaagc agcggattta gttggggttt cttctcaggc tatcagagat
    10981 gccgagaaag cagggcgact accgcacccg gatatggaaa ttcgaggacg ggttgagcaa
    11041 cgtgttggtt atacaattga acaaattaat catatgcgtg atgtgtttgg tacgcgattg
    11101 cgacgtgctg aagacgtatt tccaccggtg atcggggttg ctgcccataa aggtggcgtt
    11161 tacaaaacct cagtttctgt tcatcttgct caggatctgg ctctgaaggg gctacgtgtt
    11221 ttgctcgtgg aaggtaacga cccccaggga acagcctcaa tgtatcacgg atgggtacca
    11281 gatcttcata ttcatgcaga agacactctc ctgcctttct atcttgggga aaaggacgat
    11341 gtcacttatg caataaagcc cacttgctgg ccggggcttg acattattcc ttcctgtctg
    11401 gctctgcacc gtattgaaac tgagttaatg ggcaaatttg atgaaggtaa actgcccacc
    11461 gatccacacc tgatgctccg actggccatt gaaactgttg ctcatgacta tgatgtcata
    11521 gttattgaca gcgcgcctaa cctgggtatc ggcacgatta atgtcgtatg tgctgctgat
    11581 gtgctgattg ttcccacgcc tgctgagttg tttgactaca cctccgcact gcagtttttc
    11641 gatatgcttc gtgatctgct caagaacgtt gatcttaaag ggttcgagcc tgatgtacgt
    11701 attttgctta ccaaatacag caatagtaat ggctctcagt ccccgtggat ggaggagcaa
    11761 attcgggatg cctggggaag catggttcta aaaaatgttg tacgtgaaac ggatgaagtt
    11821 ggtaaaggtc agatccggat gagaactgtt tttgaacagg ccattgatca acgctcttca
    11881 actggtgcct ggagaaatgc tctttctatt tgggaacctg tctgcaatga aattttcgat
    11941 cgtctgatta aaccacgctg ggagattaga taatgaagcg tgcgcctgtt attccaaaac
    12001 atacgctcaa tactcaaccg gttgaagata cttcgttatc gacaccagct gccccgatgg
    12061 tggattcgtt aattgcgcgc gtaggagtaa tggctcgcgg taatgccatt actttgcctg
    12121 tatgtggtcg ggatgtgaag tttactcttg aagtgctccg gggtgatagt gttgagaaga
    12181 cctctcgggt atggtcaggt aatgaacgtg accaggagct gcttactgag gacgcactgg
    12241 atgatctcat cccttctttt ctactgactg gtcaacagac accggcgttc ggtcgaagag
    12301 tatctggtgt catagaaatt gccgatggga gtcgccgtcg taaagctgct gcacttaccg
    12361 aaagtgatta tcgtgttctg gttggcgagc tggatgatga gcagatggct gcattatcca
    12421 gattgggtaa cgattatcgc ccaacaagtg cttatgaacg tggtcagcgt tatgcaagcc
    12481 gattgcagaa tgaatttgct ggaaatattt ctgcgctggc tgatgcggaa aatatttcac
    12541 gtaagattat tacccgctgt atcaacaccg ccaaattgcc taaatcagtt gttgctcttt
    12601 tttctcaccc cggtgaacta tctgcccggt caggtgatgc acttcaaaaa gcctttacag
    12661 ataaagagga attacttaag cagcaggcat ctaaccttca tgagcagaaa aaagctgggg
    12721 tgatatttga agctgaagaa gttatcactc ttttaacttc tgtgcttaaa acgtcatctg
    12781 catcaagaac tagtttaagc tcacgacatc agtttgctcc tggagcgaca gtattgtata
    12841 agggcgataa aatggtgctt aacctggaca ggtctcgtgt tccaactgag tgtatagaga
    12901 aaattgaggc cattcttaag gaacttgaaa agccagcacc ctgatgcgac cacgttttag
    12961 tctacgttta tctgtcttta cttaatgtcc tttgttacag gccagaaagc ataactggcc
    13021 tgaatattct ctctgggccc actgttccac ttgtatcgtc ggtctgataa tcagactggg
    13081 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
    13141 atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgataat
    13201 cagactggga ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccatggt
    13261 cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg
    13321 tctgattatt agtctggaac cacggtccca ctcgtatcgt cggtctgatt attagtctgg
    13381 gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg atcccactcg
    13441 tgttgtcggt ctgattatcg gtctgggacc acggtcccac ttgtattgtc gatcagacta
    13501 tcagcgtgag actacgattc catcaatgcc tgtcaagggc aagtattgac atgtcgtcgt
    13561 aacctgtaga acggagtaac ctcggtgtgc ggttgtatgc ctgctgtgga ttgctgctgt
    13621 gtcctgctta tccacaacat tttgcgcacg gttatgtgga caaaatacct ggttacccag
    13681 gccgtgccgg cacgttaacc gggctgcatc cgatgcaagt gtgtcgctgt cgacgagctc
    13741 gcgagctcgg acatgaggtt gccccgtatt cagtgtcgct gatttgtatt gtctgaagtt
    13801 gtttttacgt taagttgatg cagatcaatt aatacgatac ctgcgtcata attgattatt
    13861 tgacgtggtt tgatggcctc cacgcacgtt gtgatatgta gatgataatc attatcactt
    13921 tacgggtcct ttccggtgat ccgacaggtt acggggcggc gacctcgcgg gttttcgcta
    13981 tttatgaaaa ttttccggtt taaggcgttt ccgttcttct tcgtcataac ttaatgtttt
    14041 tatttaaaat accctctgaa aagaaaggaa acgacaggtg ctgaaagcga gctttttggc
    14101 ctctgtcgtt tcctttctct gtttttgtcc gtggaatgaa caatggaagt ccgagctcat
    14161 cgctaataac ttcgtatagc atacattata cgaagttata ttcgat
//
