LOCUS       40924_26834        6879 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mariner transposon vector pKKma, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Zhang C.
  TITLE     Characterization of a mariner transposon pKKma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Zhang C.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUN-2014) Biological Engineering, Tianjin University 
            of Science and Technology, Thirteen Avenue of Economic Development, 
            Tianjin 300457, China
REFERENCE   3  (bases 1 to 6879)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6879)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-JUN-2014) Biological Engineering, Tianjin University of Science 
            and Technology, Thirteen Avenue of Economic Development, Tianjin 
            300457, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(91..479)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(415..1041)
                     /codon_start=1
                     /gene="traI"
                     /product="conjugal transfer relaxase"
                     /label=traI
                     /protein_id="AIN44219.1"
                     /translation="MRSIKKSDFAELVKYITDEQGKTERLGHVRVTNCEANTLPAVMAE
                     VMATQHGNTRSEADKTYHLLVSFRAGEKPDAETLRAIEDRICAGLGFAEHQRVSAVHHD
                     TDNLHIHIAINKIHPTRNTIHEPYRAYRALADLCATLERDYGLERDNHETRQRVSENRA
                     NDMERHAGVESLVGWIRPRCVRRRGSEDQQFNLLIVRTKLSCFTY"
     gene            complement(415..1041)
                     /gene="traI"
                     /label=traI
     CDS             complement(1100..1468)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(1501..1610)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_feature    1836..2231
                     /note="similar to TraK; oriT binding protein"
     primer_bind     complement(2229..2245)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2276..2380
                     /label=AmpR promoter
     CDS             2381..3238
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             3567..4613
                     /codon_start=1
                     /gene="tnpA"
                     /product="transposae"
                     /label=tnpA
                     /protein_id="AIN44221.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     gene            3567..4613
                     /gene="tnpA"
                     /label=tnpA
     promoter        complement(4675..4693)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4703..4719)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   4851..4877
     CDS             complement(5056..5586)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(5775..5803)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        6504..6532
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    6540..6556
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     repeat_region   6612..6638
ORIGIN
        1 ggccaaggct ttctgtccta gagaccgggg acttatcagc caacctgtta ctcccgggac
       61 ccagctttct tgtacagggc acaattccca tgtcagccgt taagtgttcc tgtgtcactc
      121 aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg cttgttgtcc
      181 acaaccgtta aaccttaaaa gctttaaaag ccttatatat tctttttttt cttataaaac
      241 ttaaaacctt agaggctatt taagttgctg atttatatta attttattgt tcaaacatga
      301 gagcttagta cgtgaaacat gagagcttag tacgttagcc atgagagctt agtacgttag
      361 ccatgagggt ttagttcgtt aaacatgaga gcttagtacg ttaaacatga gagcttagta
      421 cgtgaaacat gagagcttag tacgtactat caacaggttg aactgctgat cttcagatcc
      481 tctacgccgg acgcatcgtg gccggatcca gccgaccagg ctttccacgc ccgcgtgccg
      541 ctccatgtcg ttcgcgcggt tctcggaaac gcgctgccgc gtttcgtgat tgtcacgctc
      601 aagcccgtag tcccgttcga gcgtcgcgca gaggtcagcg agagcgcggt aggcccgata
      661 cggctcatgg atggtgtttc gggtcgggtg aatcttgttg atggcgatat ggatgtgcag
      721 gttgtcggtg tcgtgatgca cggcactgac gcgctgatgc tcggcgaagc caagcccagc
      781 gcagatgcgg tcctcaatcg cgcgcaacgt ctccgcgtcg ggcttctctc ccgcgcggaa
      841 gctaaccagc aggtgatagg tcttgtcggc ctcggaacgg gtgttgccgt gctgggtcgc
      901 catcacctcg gccatgacag cgggcagggt gtttgcctcg cagttcgtga cgcgcacgtg
      961 acccaggcgc tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc
     1021 gctcttcttg atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt
     1081 ttagcggcta aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc
     1141 caagctcgtc ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga
     1201 accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca
     1261 ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattc
     1321 tgtagccctg gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct
     1381 tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct
     1441 tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag
     1501 ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag
     1561 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc
     1621 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
     1681 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg
     1741 aaaagcgctg cttccctgct gttttgtgga atatctaccg actggaaaca ggcaaatgcg
     1801 ggaaattact gaactgaggg gacaggcgag agacgatgcc aaagagctac accgacgagc
     1861 tggccgagtg ggttgaatcc cgcgcggcca agaagcgccg gcgtgatgag gctgcggttg
     1921 cgttcctggc ggtgagggcg gatgtcgagg cggcgttagc gtccggctat gcgctcgtca
     1981 ccatttggga gcacatgcgg gaaacgggga aggtcaagtt ctcctacgag acgttccgct
     2041 cgcacgccag gcggcacatc aaggccaagc ccgccgatgt gcccgcaccg caggccaagg
     2101 ctgcggaacc cgcgccggca cccaagacgc cggagccacg gcggccgaag caggggggca
     2161 aggctgaaaa gccggccccc gctgcggccc cgaccggctt caccttcaac ccaacaccgg
     2221 acaaaaagga tccactagtt ctagagcggc cgctggcact tttcggggaa atgtgcgcgg
     2281 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2341 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2401 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2461 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2521 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2581 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2641 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2701 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2761 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2821 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2881 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2941 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3001 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3061 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3121 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3181 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3241 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3301 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3361 gttttcgttc cactgagcgt cagaccccgt agagcggccg ccagtgtgat ggatgacaca
     3421 tagatggcgt cgctagtatt agatgcatat tatttttata tagtaccaac cttcaaatga
     3481 ttcgtgtcaa aatttgacgt ctgtaagtca attagtttgt gagatagagc gtcttttgtg
     3541 aagcaacttt tgttattgtg aaacatatgg aaaaaaagga atttcgtgtt ttgataaaat
     3601 actgttttct gaagggaaaa aatacagtgg aagcaaaaac ttggcttgat aatgagtttc
     3661 cggactctgc cccagggaaa tcaacaataa ttgattggta tgcaaaattc aagcgtggtg
     3721 aaatgagcac ggaggacggt gaacgcagtg gacgcccgaa agaggtggtt accgacgaaa
     3781 acatcaaaaa aatccacaaa atgattttga atgaccgtaa aatgaagttg atcgagatag
     3841 cagaggcctt aaagatatca aaggaacgtg ttggtcatat cattcatcaa tatttggata
     3901 tgcggaagct ctgtgcgaaa tgggtgccgc gcgagctcac atttgaccaa aaacaacgac
     3961 gtgttgatga ttctaagcgg tgtttgcagc tgttaactcg taatacaccc gagtttttcc
     4021 gtcgatatgt gacaatggat gaaacatggc tccatcacta cactcctgag tccaatcgac
     4081 agtcggctga gtggacagcg accggtgaac cgtctccgaa gcgtggaaag actcaaaagt
     4141 ccgctggcaa agtaatggcc tctgtttttt gggatgcgca tggaataatt tttatcgatt
     4201 atcttgagaa gggaaaaacc atcaacagtg actattatat ggcgttattg gagcgtttga
     4261 aggtcgaaat cgcggcaaaa cggccccaca tgaagaagaa aaaagtgttg ttccaccaag
     4321 acaacgcacc gtgccacaag tcattgagaa cgatggcaaa aattcatgaa ttgggcttcg
     4381 aattgcttcc ccacccgccg tattctccag atctggcccc cagcgacttt ttcttgttct
     4441 cagacctcaa aaggatgctc gcagggaaaa aatttggctg caatgaagag gtgatcgccg
     4501 aaactgaggc ctattttgag gcaaaaccga aggagtacta ccaaaatggt atcaaaaaat
     4561 tggaaggtcg ttataatcgt tgtatcgctc ttgaagggaa ctatgttgaa taataaaaac
     4621 gaattttcac aaaaaaatgt gtttttcttt gttagaccgg atcctccaat tcgccctata
     4681 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca gcgtcgtgac tgggaaaacc
     4741 cttgcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4801 gcgaagaggc ccgcaccggt gccctgtaca agaaagctgg gtcccgggag taacaggttg
     4861 gctgataagt ccccggtctc tagaaagcag gcttttagac gcatcccgtt ccatacmsrm
     4921 sccgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc acttcatccg
     4981 gggtcagcac caccggcaag cgccgcgacg gcccgcgtcg gccgggaagc cgatctcggc
     5041 ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
     5101 tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta
     5161 gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc
     5221 aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg
     5281 cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc
     5341 gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc
     5401 cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc
     5461 agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact
     5521 tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg
     5581 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     5641 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     5701 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     5761 gctacttgca ttacagttta cgaaccgaac aggcttatgt caattcgaga attgacgcgc
     5821 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     5881 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     5941 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
     6001 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatac catgaaaggc
     6061 tggctttttc ttgttatcgc aatagttggc gaagtaatcg caacatccgc attaaaatct
     6121 agcgagggct ttactaagct gatccggtgg atgacctttt gaatgacctt taatagatta
     6181 tattactaat taattgggga ccctagaggt cccctttttt attttaaaaa ttttttcaca
     6241 aaacggttta caagcataaa gcttgcatgc ctgcaggtcg acggatcccc gggaatctct
     6301 agaattctca tgtttgacag cttatcatcg actgcacggt gcaccaatgc ttctggcgtc
     6361 aggcagccat cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg
     6421 tcgctcaagg cgcactcccg ttctggataa tgttttttgc gccgacatca taacggttct
     6481 ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat
     6541 tgtgagcgga taacaatttc acacaggaaa caggactcta gaggatcacc cagctttctt
     6601 gtacactaga gaccggggac ttatcagcca acctgttact cccgggaccc agctttcttg
     6661 tacagggcac aattcccatg tcagccgtta agtgttcctg tgtcactcaa aattgctttg
     6721 agaggctcta agggcttctc agtgcgttac atccctggct tgttgtccac aaccgtcaaa
     6781 ccttaaaagc tttaaaagcc ttatatactc ttttttttct tattacactt aaaaccttag
     6841 aggctattta attgccgatt tatattaatt ttattgttc
//
