LOCUS       40924_26824        5075 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKK388-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Brosius J.
  TITLE     Expression vectors employing lambda-, trp-, lac- and lpp-derived 
            promoters
  JOURNAL   (in) Rodriguez,R.L. and Denhardt,D.T. (Eds.); VECTORS: 205-225; 
            (1988)
REFERENCE   3  (bases 1 to 5075)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 5075)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5075)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "(in) 
            Rodriguez,R.L. and Denhardt,D.T. (Eds.)"; volume: " VECTORS"; pages:
            " 205-225; (1988"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor.  For technical 
            information, call (415) 424- 8222 or (800) 662-2566, extension 3. 
            This sequence was compiled by Jurgen Brosius. If you suspect there 
            is an error in this sequence, please contact CLONTECH's Technical 
            Service Department at (415) 424-8222 or (800) 662-2566, extension 3 
            or E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..5075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        163..192
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    200..216
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    233..302
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     RBS             316..324
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    341..397
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      600..686
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      778..805
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        825..916
                     /label=AmpR promoter
     CDS             917..1774
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1948..2536
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2722..2862)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2967..3155)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3797..4984)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        complement(5023..5053)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
ORIGIN
        1 ggccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta
       61 aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg
      121 ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatccg
      181 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagcgccgct
      241 gagaaaaagc gaagcggcac tgctctttaa caatttatca gacaatctgt gtgggcactc
      301 gaccggaatt atcgataagg aggtttaaac catggcagct gaattcgagc tcggtacccg
      361 gggatcctct agagtcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa
      421 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
      481 gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
      541 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
      601 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      661 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
      721 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
      781 aggccatcct gacggatggc ctttttgcgt ttctacaaac tctttttgtt tatttttcta
      841 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      901 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      961 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1021 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1081 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1141 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1201 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1261 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1321 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1381 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1441 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1501 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1561 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1621 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1681 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1741 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2641 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2701 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2761 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2821 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2881 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2941 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     3001 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     3061 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     3121 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     3181 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     3241 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     3301 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     3361 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     3421 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     3481 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     3541 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     3601 agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg
     3661 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     3721 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     3781 cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga
     3841 ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg
     3901 aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag
     3961 ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg
     4021 cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca
     4081 tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg
     4141 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag
     4201 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc
     4261 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca
     4321 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
     4381 agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct
     4441 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     4501 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     4561 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     4621 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     4681 cggcgtagag gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc
     4741 caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa
     4801 cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg
     4861 cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc
     4921 ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt
     4981 tcatacacgg tgcctgactg cgttagccca agctattccg cgcattatac gagccggaag
     5041 cataaagtgt aaagcttgtg caggtcgacg gatcc
//
