LOCUS       V005223                 4040 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005223
VERSION     V005223
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Krute CN, Krausz KL, Markiewicz MA, Joyner JA, Pokhrel S, Hall PR,
            Bose JL.
  TITLE     Generation of a stable plasmid for in vitro and in vivo studies of
            Staphylococcus
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
   PUBMED   27637878
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Krausz KL, Krute CN, Markiewicz MA, Bose JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2016) Microbiology, Molecular Genetics and
            Immunology, University of Kansas Medical Center, 3901 Rainbow Blvd,
            Kansas City, KS 66160, USA
REFERENCE   3  (bases 1 to 4040)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4040)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-APR-2016) Microbiology, Molecular Genetics and Immunology,
            University of Kansas Medical Center, 3901 Rainbow Blvd, Kansas City,
            KS 66160, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label="repF"
                     /note="replication protein"
                     /protein_id="AOR52299.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label="repF"
     rep_origin      1179..1567
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /label="sarA P1 promoter"
                     /note="sarA P1 promoter"
                     /regulatory_class="promoter"
     CDS             1847..2329
                     /gene="dfrA"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Tn4003 from
                     Staphylococcus aureus. Accession#: P13955"
     regulatory      2340..2639
                     /label="blaZ transcription terminator"
                     /note="blaZ transcription terminator"
                     /regulatory_class="terminator"
     regulatory      2766..2823
                     /label="atl transcription terminator"
                     /note="atl transcription terminator"
                     /regulatory_class="terminator"
     rep_origin      3220..3397
                     /label="sso"
                     /note="sso"
     CDS             complement(3618..3629)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     rep_origin      3866..3886
                     /gene="dso"
     gene            3866..3886
                     /gene="dso"
                     /label="dso"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 aataagacaa acgcaatata ttgtgtcaca aaaataagac agtacagctt tgtatgatcc
     1141 gtcgacgaaa gcctggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
     1201 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
     1261 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
     1321 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
     1381 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
     1441 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
     1501 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg
     1561 gctgacatgg gaattcgagc tgatattttt gactaaacca aatgctaacc cagaaataca
     1621 atcactgtgt ctaatgaata atttgtttta taaacacttt tttgtttact tctcattttt
     1681 aattagttat aattaactaa ataatagagc attaaatata tttaataaaa cttatttaat
     1741 gcaaaattat gactaacata tctataataa ataaagatta gatatcaata tattatcggg
     1801 caaatgtatc gagcaagatg catcggatcg atccaggagg tataccatga cattatcaat
     1861 aattgtcgct cacgataaac aaagagtcat tgggtaccaa aatcaattac cttggcactt
     1921 accaaatgat ttaaagcata ttaaacaact gaccactggg aatacacttg taatggcacg
     1981 gaaaactttt aattctatag ggaagccatt gccaaataga cgtaacgtcg tactcactaa
     2041 ccaagcttca tttcgccatg aaggggtaga tgttataaac tctcttgatg aaattaaaga
     2101 gttatctggt catgttttta tatttggagg acaaacgtta tacgaagcaa tgattgacca
     2161 ggtagatgat atgtatatca cagtaataga tggaaagttt caaggagaca cattctttcc
     2221 accatacaca ttcgaaaact gggaagtcga atcttcagta gaaggtcaac tagatgaaaa
     2281 aaatactata ccgcatacat tcttacattt agtgcgtaga aaagggaaat aggcgcgcct
     2341 attctaatgc ataataaata ctgataacat cttatatttt gtattatatt ttgtattatc
     2401 gttgacatgt ataattttga tatcaaaaac tgattttccc tctattattt tcgagattta
     2461 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatt ataaataata
     2521 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt
     2581 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag
     2641 gcgatgcggc cgctagccta ggagctcggt acccggggat ccgaatcatg aattacaagc
     2701 aaaagtagcg gtgattgtta aaattgatgg taaacaatca ccgctatttt tgcttgtgta
     2761 tgtataaaaa agggatcaaa ggtcatcccc catgattgat agtgggggga tgacttttga
     2821 tcctatgttc atgttgctta tttaatcgcc tttgatcact ttaaaatacc ttaaaacccc
     2881 tggaatttct ggctttgcca gacctatcat ttttgaatga tagcaaattc tccttatgct
     2941 cttacggagt ttttagagaa aaattaaaaa ttctcgattt ttgataaaaa acgccctgca
     3001 ggaatttaga aaaacatgtg gaagttttaa aggattttat gctaattttt aatttgcatg
     3061 taactcgagg ggaatatttg aggggatttt gaaacgagtt tcttcttgtt ttcacactgt
     3121 ttttttattc ctattggtgt tgttgcttac tttttgtttt tctataaaga tgatgctttt
     3181 gacgagatgg aagaaaaata tgattatcat gaagataata aaaaatagac gacgcattta
     3241 tgccgagaaa atttattgat gttgagaaga acccttaact aaacttgcag acgaatgtcg
     3301 gcatagcgtg agctattaag ccgaccattc gacaagtttt gggattgtta agggttccga
     3361 ggctcaacgt caataaagca attggaataa atagtatcta aagtaacccg tatttaagat
     3421 tacattgtac atccttcgaa accatagtag ctgcgattcc agctaggttt ccttttatcc
     3481 caattttagc taattttttt gctacgccct ttaagttttt ggctttaaca tcttcaaaaa
     3541 ctgctgtgat tatgtttcca gttaaaagtt ctccatatga gtttaaaact tcagaataga
     3601 aacagtcacc gctatttttg tagttcccac caactgctct aggtgatatt gaactagttt
     3661 tctttttatt ctctatgtcg ttttttagtt tttggaatcc ttcaggaaca tatccatatc
     3721 gagatttggc cgattttatt tgataatcct tttgactttt tagaaagttt tatttattca
     3781 ttggttttca cattaattta tttaggttta gagggtcata aaaagaagaa ggaataggtt
     3841 gttttttgaa acgagtgtga acgagtttct tcttgtcttg atactatata gaaataactc
     3901 gattttatat atatagctgt aactgttgat attacagtgt ttaaacgtgt ttttgtgcgt
     3961 gaaaggaaaa tttgacaata aaaaacccca gttatattat taaggtgtcg aatcttaaat
     4021 aatactgggg gtctttttat
//
