LOCUS       Exported                4585 bp DNA     circular SYN 21-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4585)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4585)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4585)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      236..322
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      414..441
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        460..551
                     /label=AmpR promoter
     CDS             552..1412
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1583..2171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2357..2497
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2602..2790)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3429..4214)
                     /codon_start=1
                     /product="Tetracycline resistance protein, class C"
                     /label=TetA(C)
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFL
                     MQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGED
                     RFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFAT
                     RGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIGPLIVTA
                     IYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        4514..4542
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4550..4566
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttcccgggga tccgtcgacc tgcagccaag cttggctgtt ttggcggatg agagaagatt
       61 ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
      121 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
      181 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
      241 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      301 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
      361 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
      421 catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac
      481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      781 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2341 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2401 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     2461 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     2521 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     2581 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     2641 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     2701 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     2761 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     2821 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     2881 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     2941 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     3001 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     3061 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     3121 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     3181 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     3241 gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct
     3301 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     3361 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg
     3421 gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag
     3481 acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg
     3541 gcataaatcg ccgtgacgat cagcggtcca atgatcgaag ttaggctggt aagagccgcg
     3601 agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     3661 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     3721 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata
     3781 atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
     3841 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
     3901 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
     3961 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
     4021 actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt
     4081 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     4141 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     4201 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     4261 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     4321 tagaggatcc gggcttatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat
     4381 cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg
     4441 cgcactcccg ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt
     4501 ctgaaatgag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga
     4561 taacaatttc acacaggaaa cagaa
//
