LOCUS       V005225                 4841 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005225
VERSION     V005225
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Krute CN, Krausz KL, Markiewicz MA, Joyner JA, Pokhrel S, Hall PR,
            Bose JL.
  TITLE     Generation of a stable plasmid for in vitro and in vivo studies of
            Staphylococcus
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
   PUBMED   27637878
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Krausz KL, Krute CN, Markiewicz MA, Bose JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2016) Microbiology, Molecular Genetics and
            Immunology, University of Kansas Medical Center, 3901 Rainbow Blvd,
            Kansas City, KS 66160, USA
REFERENCE   3  (bases 1 to 4841)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-APR-2016) Microbiology, Molecular Genetics and Immunology,
            University of Kansas Medical Center, 3901 Rainbow Blvd, Kansas City,
            KS 66160, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /function="replication"
                     /label="repF"
                     /note="replication protein"
                     /protein_id="AOR52293.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label="repF"
     rep_origin      1179..1567
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /label="sarA P1 promoter"
                     /note="sarA P1 promoter"
                     /regulatory_class="promoter"
     CDS             1847..2329
                     /gene="dfrA"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Tn4003 from
                     Staphylococcus aureus. Accession#: P13955"
     regulatory      2340..2639
                     /label="blaZ transcriptional terminator"
                     /note="blaZ transcriptional terminator"
                     /regulatory_class="terminator"
     regulatory      2766..2823
                     /gene="atl"
                     /regulatory_class="terminator"
     gene            2766..2823
                     /gene="atl"
                     /label="atl"
     CDS             3064..3180
                     /codon_start=1
                     /gene="cds2"
                     /product="hypothetical protein"
                     /label="cds2"
                     /protein_id="AOR52295.1"
                     /translation="MGSVKKLVTGFAEIFVCEITIYLFGSYLRFKSLYIFLS"
     gene            3064..3180
                     /gene="cds2"
                     /label="cds2"
     CDS             3262..3483
                     /codon_start=1
                     /gene="cds3"
                     /product="hypothetical protein"
                     /label="cds3"
                     /protein_id="AOR52296.1"
                     /translation="MIKFFKKGVFILNKNTVLDEGYVIATILNVFFLLGLIFISHLENL
                     YILIPYVILMGINAIYLVVKVMNFKKNN"
     gene            3262..3483
                     /gene="cds3"
                     /label="cds3"
     rep_origin      3631..3808
                     /label="sso"
                     /note="sso"
     CDS             complement(4029..4040)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     CDS             4465..4638
                     /codon_start=1
                     /gene="cds5"
                     /product="hypothetical protein"
                     /label="cds5"
                     /protein_id="AOR52298.1"
                     /translation="MNKFFYFLIILLSVFLIFNFVNSILFDNPFDFLESFIYSLVFTLI
                     YLGLEGHKKKKE"
     gene            4465..4638
                     /gene="cds5"
                     /label="cds5"
     rep_origin      4667..4687
                     /label="dso"
                     /note="dso"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 aataagacaa acgcaatata ttgtgtcaca aaaataagac agtacagctt tgtatgatcc
     1141 gtcgacgaaa gcctggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
     1201 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
     1261 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
     1321 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
     1381 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
     1441 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
     1501 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg
     1561 gctgacatgg gaattcgagc tgatattttt gactaaacca aatgctaacc cagaaataca
     1621 atcactgtgt ctaatgaata atttgtttta taaacacttt tttgtttact tctcattttt
     1681 aattagttat aattaactaa ataatagagc attaaatata tttaataaaa cttatttaat
     1741 gcaaaattat gactaacata tctataataa ataaagatta gatatcaata tattatcggg
     1801 caaatgtatc gagcaagatg catcggatcg atccaggagg tataccatga cattatcaat
     1861 aattgtcgct cacgataaac aaagagtcat tgggtaccaa aatcaattac cttggcactt
     1921 accaaatgat ttaaagcata ttaaacaact gaccactggg aatacacttg taatggcacg
     1981 gaaaactttt aattctatag ggaagccatt gccaaataga cgtaacgtcg tactcactaa
     2041 ccaagcttca tttcgccatg aaggggtaga tgttataaac tctcttgatg aaattaaaga
     2101 gttatctggt catgttttta tatttggagg acaaacgtta tacgaagcaa tgattgacca
     2161 ggtagatgat atgtatatca cagtaataga tggaaagttt caaggagaca cattctttcc
     2221 accatacaca ttcgaaaact gggaagtcga atcttcagta gaaggtcaac tagatgaaaa
     2281 aaatactata ccgcatacat tcttacattt agtgcgtaga aaagggaaat aggcgcgcct
     2341 attctaatgc ataataaata ctgataacat cttatatttt gtattatatt ttgtattatc
     2401 gttgacatgt ataattttga tatcaaaaac tgattttccc tctattattt tcgagattta
     2461 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatt ataaataata
     2521 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt
     2581 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag
     2641 gcgatgcggc cgctagccta ggagctcggt acccggggat ccgaatcatg aattacaagc
     2701 aaaagtagcg gtgattgtta aaattgatgg taaacaatca ccgctatttt tgcttgtgta
     2761 tgtataaaaa agggatcaaa ggtcatcccc catgattgat agtgggggga tgacttttga
     2821 tcctatgttc atgttgctta tttaatcgcc tttgatcact ttaaaatacc ttaaaacccc
     2881 tggaatttct ggctttgcca gacctatcat ttttgaatga tagcaaattc tccttatgct
     2941 cttacggagt ttttagagaa aaattaaaaa ttctcgattt ttgataaaaa acgccctgca
     3001 ggaatttaga aaaacatgtg gaagttttaa aggattttat gctaattttt aatttgcatg
     3061 taaatgggca gtgttaaaaa attagtcact ggttttgctg aaatttttgt ttgtgaaatt
     3121 accatatatt tatttggctc atatttgcgt tttaagagct tgtatatatt tttaagttaa
     3181 aaacctatat gataccccct aaacctttaa aataccccct taaaactcaa aataaaggta
     3241 tttataattt gagtaagtct tgtgataaag ttttttaaaa aaggagtgtt tattttgaat
     3301 aaaaacacag tgttagatga aggttacgtt atagcaacta ttttaaatgt tttcttttta
     3361 cttggattaa tttttatatc tcatttggaa aatttatata tattaattcc gtatgttatt
     3421 ttgatgggaa taaatgctat ttatttggtt gttaaggtta tgaatttcaa aaagaataat
     3481 tagaatattt gaggggattt tgaaacgagt ttcttcttgt tttcacactg tttttttatt
     3541 cctattggtg ttgttgctta ctttttgttt ttctataaag atgatgcttt tgacgagatg
     3601 gaagaaaaat atgattatca tgaagataat aaaaaataga cgacgcattt atgccgagaa
     3661 aatttattga tgttgagaag aacccttaac taaacttgca gacgaatgtc ggcatagcgt
     3721 gagctattaa gccgaccatt cgacaagttt tgggattgtt aagggttccg aggctcaacg
     3781 tcaataaagc aattggaata aatagtatct aaagtaaccc gtatttaaga ttacattgta
     3841 catccttcga aaccatagta gctgcgattc cagctaggtt tccttttatc ccaattttag
     3901 ctaatttttt tgctacgccc tttaagtttt tggctttaac atcttcaaaa actgctgtga
     3961 ttatgtttcc agttaaaagt tctccatatg agtttaaaac ttcagaatag aaacagtcac
     4021 cgctattttt gtagttccca ccaactgctc taggtgatat tgaactagtt ttctttttat
     4081 tctctatgtc gttttttagt ttttggaatc cttcaggaac atatccatat cgagatttgg
     4141 cattttctag attaacatct ttagctgttc cgtctttatc taaaataata attttgccat
     4201 atatgtattt tagttctttt tcaatttctt tttcgtttaa agtttcaacg tttttatctg
     4261 caatgttatt tttgttttgg gcttgagctt ctttgaaatt aaaatccaag accattgaaa
     4321 gtatggttaa aatacttaaa gatactacta aaatctttct tagtgatata ctgttttgaa
     4381 ttgcattcat tgtaaatgcc cccttgtttt gttatttaaa tgaattcaat ataatattaa
     4441 catataaaaa caaaggagtt taatatgaat aagttttttt actttttgat tattttatta
     4501 tctgtatttt taatttttaa ttttgttaat tcgattttat ttgataatcc ttttgacttt
     4561 ttagaaagtt ttatttattc attggttttc acattaattt atttaggttt agagggtcat
     4621 aaaaagaaga aggaataggt tgttttttga aacgagtgtg aacgagtttc ttcttgtctt
     4681 gatactatat agaaataact cgattttata tatatagctg taactgttga tattacagtg
     4741 tttaaacgtg tttttgtgcg tgaaaggaaa atttgacaat aaaaaacccc agttatatta
     4801 ttaaggtgtc gaatcttaaa taatactggg ggtcttttta t
//
