LOCUS       V015933                 5661 bp    DNA     circular SYN 15-FEB-2020
DEFINITION  Exported.
ACCESSION   V015933
VERSION     V015933
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Krawczyk K, Scheller L, Kim H, Fussenegger M.
  TITLE     Rewiring of endogenous signaling pathways to genomic targets for
            therapeutic cell reprogramming
  JOURNAL   Nat Commun 11 (1), 608 (2020)
   PUBMED   32001704
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Krawczyk KK.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
            4058, Switzerland
REFERENCE   3  (bases 1 to 5661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "608"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
            Switzerland"
FEATURES             Location/Qualifiers
     source          1..5661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             128..919
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1096..1229
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1266..1282)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1290..1306)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1314..1344)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1359..1380)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1668..2253)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2427..3284)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3285..3389)
                     /label="AmpR promoter"
     rep_origin      3495..3630
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             3673..4620
                     /gene="NFKBIA"
                     /label="NF-kappa-B inhibitor alpha"
                     /note="NF-kappa-B inhibitor alpha from Homo sapiens.
                     Accession#: P25963"
     polyA_signal    4681..4905
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4951..5379
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5393..5661
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
       61 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
      121 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
      181 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
      241 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
      301 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
      361 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
      421 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
      481 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
      541 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
      601 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
      661 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
      721 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
      781 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
      841 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
      901 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
      961 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     1021 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     1081 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1201 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     1261 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1321 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1381 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1441 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1501 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1561 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1681 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1741 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1861 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1981 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2041 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2101 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2161 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag
     2221 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2281 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2341 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2401 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2461 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2521 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2581 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2641 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2701 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2761 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2821 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2881 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2941 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3001 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3061 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3301 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3361 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3421 gacggatcgg gagatctcca cgcgtggtac cctcgaggat ctgcgatctg catctcaatt
     3481 agtcagcaac catagtcccg cccctaactc cgcccagttc cgcccattct ccgccccatc
     3541 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3601 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaattcga agcggaattc
     3661 accatgacta gtttccaggc ggccgagcgc ccccaggagt gggccatgga gggcccccgc
     3721 gacgggctga agaaggagcg gctactggac gaccgccacg acagcggcct ggactccatg
     3781 aaagacgagg agtacgagca gatggtcaag gagctgcagg agatccgcct cgagccgcag
     3841 gaggtgccgc gcggctcgga gccctggaag cagcagctca ccgaggacgg ggactcgttc
     3901 ctgcacttgg ccatcatcca tgaagaaaag gcactgacca tggaagtgat ccgccaggtg
     3961 aagggagacc tggccttcct caacttccag aacaacctgc agcagactcc actccacttg
     4021 gctgtgatca ccaaccagcc agaaattgct gaggcacttc tgggagctgg ctgtgatcct
     4081 gagctccgag actttcgagg aaataccccc ctacaccttg cctgtgagca gggctgcctg
     4141 gccagcgtgg gagtcctgac tcagtcctgc accaccccgc acctccactc catcctgaag
     4201 gctaccaact acaatggcca cacgtgtcta cacttagcct ctatccatgg ctacctgggc
     4261 atcgtggagc ttttggtgtc cttgggtgct gatgtcaatg ctcaggagcc ctgtaatggc
     4321 cggactgccc ttcacctcgc agtggacctg caaaatcctg acctggtgtc actcctgttg
     4381 aagtgtgggg ctgatgtcaa cagagttacc taccagggct attctcccta ccagctcacc
     4441 tggggccgcc caagcacccg gatacagcag cagctgggcc agctgacact agaaaacctt
     4501 cagatgctgc cagagagtga ggatgaggag agctatgaca cagagtcaga gttcacggag
     4561 ttcacagagg acgagctgcc ctatgatgac tgtgtgtttg gaggccagcg tctgacgtta
     4621 gctagcggat ccaccggtgt ctagaaagct ttgaggccgg cctgcaggga tcagcctcga
     4681 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4741 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4801 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4861 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4921 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4981 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5041 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5101 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5161 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5221 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5281 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5341 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5401 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5461 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5521 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5581 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5641 tgcagaggcc gaggccgcct c
//
