LOCUS       V016094                 7452 bp    DNA     circular SYN 15-FEB-2020
DEFINITION  Exported.
ACCESSION   V016094
VERSION     V016094
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Krawczyk K, Scheller L, Kim H, Fussenegger M.
  TITLE     Rewiring of endogenous signaling pathways to genomic targets for
            therapeutic cell reprogramming
  JOURNAL   Nat Commun 11 (1), 608 (2020)
   PUBMED   32001704
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Krawczyk KK.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
            4058, Switzerland
REFERENCE   3  (bases 1 to 7452)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "608"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
            Switzerland"
FEATURES             Location/Qualifiers
     source          1..7452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             128..919
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1096..1229
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1266..1282)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1290..1306)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1314..1344)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1359..1380)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1668..2253)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2427..3284)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3285..3389)
                     /label="AmpR promoter"
     rep_origin      3495..3630
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     misc_feature    3664..4024
                     /gene="GEAR p65"
                     /label="MCP"
                     /note="MCP"
     CDS             4042..4755
                     /label="YPet"
                     /note="yellow fluorescent protein optimized for FRET with
                     CyPet (Nguyen and Daugherty, 2005)"
     CDS             4771..6417
                     /gene="RELA"
                     /label="Transcription factor p65"
                     /note="Transcription factor p65 from Homo sapiens.
                     Accession#: Q04206"
     polyA_signal    6472..6696
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6742..7170
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7184..7452
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
       61 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
      121 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
      181 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
      241 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
      301 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
      361 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
      421 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
      481 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
      541 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
      601 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
      661 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
      721 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
      781 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
      841 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
      901 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
      961 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     1021 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     1081 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1201 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     1261 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1321 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1381 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1441 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1501 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1561 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1681 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1741 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1861 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1981 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2041 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2101 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2161 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag
     2221 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2281 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2341 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2401 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2461 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2521 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2581 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2641 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2701 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2761 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2821 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2881 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2941 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3001 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3061 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3301 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3361 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3421 gacggatcgg gagatctcca cgcgtggtac cctcgaggat ctgcgatctg catctcaatt
     3481 agtcagcaac catagtcccg cccctaactc cgcccagttc cgcccattct ccgccccatc
     3541 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3601 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaattcga agcggaattc
     3661 accatgacta gtgcttctaa ctttactcag ttcgttctcg tcgacaatgg cggaactggc
     3721 gacgtgactg tcgccccaag caacttcgct aacgggatcg ctgaatggat cagctctaac
     3781 tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc agagctctgc gcagaatcgc
     3841 aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc gttcgtactt aaatatggaa
     3901 ctaaccattc caattttcgc cacgaattcc gactgcgagc ttattgttaa ggcaatgcaa
     3961 ggtctcctaa aagatggaaa cccgattccc tcagcaatcg cagcaaactc cggcatctac
     4021 gcggctagtg gtggttctgg tgtgagcaaa ggcgaagagc tgttcaccgg cgtggtgccc
     4081 atcctggtgg agctggacgg cgacgtgaac ggccacaagt tcagcgtgag cggcgagggc
     4141 gagggcgacg ccacctacgg caagctgacc ctgaagctgc tgtgcaccac cggcaagctg
     4201 cccgtgccct ggcccaccct ggtgaccacc ctgggctacg gcgtgcagtg cttcgcccgg
     4261 taccccgacc acatgaagca gcacgacttc ttcaagagcg ccatgcccga gggctacgtg
     4321 caggagcgga ccatcttctt caaggacgac ggcaactaca agacccgggc cgaggtgaag
     4381 ttcgagggcg acaccctggt gaaccggatc gagctgaagg gcatcgactt caaggaggac
     4441 ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt gtacatcacc
     4501 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccggcacaa catcgaggac
     4561 ggcggcgtgc agctggccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4621 ctgctgcccg acaaccacta cctgagctac cagagcgccc tgttcaagga ccccaacgag
     4681 aagcgggacc acatggtgct gctggagttc ctgaccgccg ccggcatcac cgagggcatg
     4741 gacgagctgt acaaggctag tggtggtgga gacgaactgt tccccctcat cttcccggca
     4801 gagccagccc aggcctctgg cccctatgtg gagatcattg agcagcccaa gcagcggggc
     4861 atgcgcttcc gctacaagtg cgaggggcgc tccgcgggca gcatcccagg cgagaggagc
     4921 acagatacca ccaagaccca ccccaccatc aagatcaatg gctacacagg accagggaca
     4981 gtgcgcatct ccctggtcac caaggaccct cctcaccggc ctcaccccca cgagcttgta
     5041 ggaaaggact gccgggatgg cttctatgag gctgagctct gcccggaccg ctgcatccac
     5101 agtttccaga acctgggaat ccagtgtgtg aagaagcggg acctggagca ggctatcagt
     5161 cagcgcatcc agaccaacaa caaccccttc caagttccta tagaagagca gcgtggggac
     5221 tacgacctga atgctgtgcg gctctgcttc caggtgacag tgcgggaccc atcaggcagg
     5281 cccctccgcc tgccgcctgt cctttctcat cccatctttg acaatcgtgc ccccaacact
     5341 gccgagctca agatctgccg agtgaaccga aactctggca gctgcctcgg tggggatgag
     5401 atcttcctac tgtgtgacaa ggtgcagaaa gaggacattg aggtgtattt cacgggacca
     5461 ggctgggagg cccgaggctc cttttcgcaa gctgatgtgc accgacaagt ggccattgtg
     5521 ttccggaccc ctccctacgc agaccccagc ctgcaggctc ctgtgcgtgt ctccatgcag
     5581 ctgcggcggc cttccgaccg ggagctcagt gagcccatgg aattccagta cctgccagat
     5641 acagacgatc gtcaccggat tgaggagaaa cgtaaaagga catatgagac cttcaagagc
     5701 atcatgaaga agagtccttt cagcggaccc accgaccccc ggcctccacc tcgacgcatt
     5761 gctgtgcctt cccgcagctc agcttctgtc cccaagccag caccccagcc ctatcccttt
     5821 acgtcatccc tgagcaccat caactatgat gagtttccca ccatggtgtt tccttctggg
     5881 cagatcagcc aggcctcggc cttggccccg gcccctcccc aagtcctgcc ccaggctcca
     5941 gcccctgccc ctgctccagc catggtatca gctctggccc aggccccagc ccctgtccca
     6001 gtcctagccc caggccctcc tcaggctgtg gccccacctg cccccaagcc cacccaggct
     6061 ggggaaggaa cgctgtcaga ggccctgctg cagctgcagt ttgatgatga agacctgggg
     6121 gccttgcttg gcaacagcac agacccagct gtgttcacag acctggcatc cgtcgacaac
     6181 tccgagtttc agcagctgct gaaccagggc atacctgtgg ccccccacac aactgagccc
     6241 atgctgatgg agtaccctga ggctataact cgcctagtga caggggccca gaggcccccc
     6301 gacccagctc ctgctccact gggggccccg gggctcccca atggcctcct ttcaggagat
     6361 gaagacttct cctccattgc ggacatggac ttctcagccc tgctgagtca gatcagcgga
     6421 tccaccggtg tctagaaagc tttgaggccg gcctgcaggg atcagcctcg actgtgcctt
     6481 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6541 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6601 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6661 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6721 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6781 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6841 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6901 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6961 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7021 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7081 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7141 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     7201 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7261 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7321 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7381 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7441 cgaggccgcc tc
//
