LOCUS       Exported                7192 bp DNA     circular SYN 10-OCT-2024
DEFINITION  Exported.
ACCESSION   V012942
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7192)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1787..3700
                     /gene="dnaK"
                     /label=Chaperone protein DnaK
                     /note="Chaperone protein DnaK from Escherichia coli O1:K1 /
                     APEC. Accession#: A1A766"
     CDS             3792..4919
                     /gene="dnaJ"
                     /label=Chaperone protein DnaJ
                     /note="Chaperone protein DnaJ from Escherichia coli (strain
                     K12). Accession#: P08622"
     CDS             4939..5529
                     /gene="grpE"
                     /label=Protein GrpE
                     /note="Protein GrpE from Escherichia coli (strain K12). 
                     Accession#: P09372"
     rep_origin      5800..6345
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        6871..6973
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             join(6974..7192,1..438)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa tagttacggc
      541 ttatgacatc tttgtggaca catcattcac tttttattca catccggccc tgaactcgct
      601 aggacttgcc ccggtgcatt ttttaaatac ccgcgaaaaa tagagctgat cgtcaaatcc
      661 aacattgcgc ccaacggtcg ctatcggcat tcgcgtagtg ctaagcagaa gtttcgcctg
      721 gctgatacgc tgatcttcgc gccagctcaa tacgctaatg cctaactgct ggcggaacag
      781 atgtgataac cgggagggcg acaggcagac atgctgggcg acgctggcga tatcaaaatg
      841 gctgtccgcc agatggtcgc tgatatactg gcaggcatcg cgcacacggc tatccatcgg
      901 cgggtgcaac gactcattaa ttaccgccat acgtctgagc aacaactgct ccagcagatt
      961 gatcgccagt agctcagaat agcgaccttc cccttgcccg gcgctgatga tctgcccgaa
     1021 cagttcgctg aaatgcggct ggcgcgcctc gtccgggcgg aaaaatcctg tctgggcaaa
     1081 gattgtcggc caggtcagcc actcctgcca gtaggcgcga ggccggaaat aaacccactg
     1141 gtgataccac tcgctggcgt ccggatgccg tccatagtga tgaatctcgc ccggcggaaa
     1201 caataatata tcgccaggcc gacagacaaa ctgctcgcca ttattattaa tgacgccctc
     1261 tccgcggatg gtcaggttaa gaatatatcc cttcatgccc aacggacgat cgataaaaaa
     1321 atccagatat ccattcgctt caattggcgt cagcccggcg accagatggg cattaaatga
     1381 atatcccggc aatagcggat cattttgcgt ttcagccatg atttctctac cccccgatgt
     1441 tcagagaaga aacaaattgt ccatatcgac caggacgaca gagcttccgt ctccgcaaga
     1501 ctttgcgctt gatgaaagca cgtatcaacc ccgcttgtga aaagcgcttt gtaacaaaag
     1561 cgtacagttc aggcgataaa attaagtaac agaagtgtct ataactatgg ctggaatgtc
     1621 cacattgaat atttgcacag cgtcacactt tgcaaagcat tagcattttt gtccataaga
     1681 ttagcggatc ctgcctgacg gtttttgccg cgactctcta taatttctcc atacctgttt
     1741 ttctggatgg agtaagacca tggctatgga ttgtggagac gtttagatgg gtaaaataat
     1801 tggtatcgac ctgggtacta ccaactcttg tgtagcgatt atggatggca ccactcctcg
     1861 cgtgctggag aacgccgaag gcgatcgcac cacgccttct atcattgcct atacccagga
     1921 tggtgaaact ctagttggtc agccggctaa acgtcaggca gtgacgaacc cgcaaaacac
     1981 tctgtttgcg attaaacgcc tgattggtcg ccgcttccag gacgaagaag tacagcgtga
     2041 tgtttccatc atgccgttca aaattattgc tgctgataac ggcgacgcat gggtcgaagt
     2101 taaaggccag aaaatggcac cgccgcagat ttctgctgaa gtgctgaaaa aaatgaagaa
     2161 aaccgctgaa gattacctgg gtgaaccggt aactgaagct gttatcaccg taccggcata
     2221 ctttaacgat gctcagcgtc aggcaaccaa agacgcaggc cgtatcgctg gtctggaagt
     2281 aaaacgtatc atcaacgaac cgaccgcagc tgcgctggct tacggtctgg acaaaggcac
     2341 tggcaaccgt actatcgcgg tttatgacct gggtggtggt actttcgata tttctattat
     2401 cgaaatcgac gaagttgacg gcgaaaaaac cttcgaagtt ctggcaacca acggtgatac
     2461 ccacctgggg ggtgaagact tcgacagccg tctgatcaac tatctggttg aagaattcaa
     2521 gaaagatcag ggcattgacc tgcgcaacga tccgctggca atgcagcgcc tgaaagaagc
     2581 ggcagaaaaa gcgaaaatcg aactgtcttc cgctcagcag accgacgtta acctgccata
     2641 catcactgca gacgcgaccg gtccgaaaca catgaacatc aaagtgactc gtgcgaaact
     2701 ggaaagcctg gttgaagatc tggtaaaccg ttccattgag ccgctgaaag ttgcactgca
     2761 ggacgctggc ctgtccgtat ctgatatcga cgacgttatc ctcgttggtg gtcagactcg
     2821 tatgccaatg gttcagaaga aagttgctga gttctttggt aaagagccgc gtaaagacgt
     2881 taacccggac gaagctgtag caatcggtgc tgctgttcag ggtggtgttc tgactggtga
     2941 cgtaaaagac gtactgctgc tggacgttac cccgctgtct ctgggtatcg aaaccatggg
     3001 cggtgtgatg acgacgctga tcgcgaaaaa caccactatc ccgaccaagc acagccaggt
     3061 gttctctacc gctgaagaca accagtctgc ggtaaccatc catgtgctgc agggtgaacg
     3121 taaacgtgcg gctgataaca aatctctggg tcagttcaac ctagatggta tcaacccggc
     3181 accgcgcggc atgccgcaga tcgaagttac cttcgatatc gatgctgacg gtatcctgca
     3241 cgtttccgcg aaagataaaa acagcggtaa agagcagaag atcaccatca aggcttcttc
     3301 tggtctgaac gaagatgaaa tccagaaaat ggtacgcgac gcagaagcta acgccgaagc
     3361 tgaccgtaag tttgaagagc tggtacagac tcgcaaccag ggcgaccatc tgctgcacag
     3421 cacccgtaag caggttgaag aagcaggcga caaactgccg gctgacgaca aaactgctat
     3481 cgagtctgcg ctgactgcac tggaaactgc tctgaaaggt gaagacaaag ccgctatcga
     3541 agcgaaaatg caggaactgg cacaggtttc ccagaaactg atggaaatcg cccagcagca
     3601 acatgcccag cagcagactg ccggtgctga tgcttctgca aacaacgcga aagatgacga
     3661 tgttgtcgac gctgaatttg aagaagtcaa agacaaaaaa taatcgccct ataaacgggt
     3721 aattatactg acacgggcga aggggaattt cctccccgcc cgtgcattca tctaggggca
     3781 atttaaaaaa gatggctaag caagattatt acgagatttt aggcgtttcc aaaacagcgg
     3841 aagagcgtga aatcagaaag gcctacaaac gcctggccat gaaataccac ccggaccgta
     3901 accagggtga caaagaggcc gaggcgaaat ttaaagagat caaggaagct tatgaagttc
     3961 tgaccgactc gcaaaaacgt gcggcatacg atcagtatgg tcatgctgcg tttgagcaag
     4021 gtggcatggg cggcggcggt tctggcggcg gcgcagactt cagcgatatt tttggtgacg
     4081 ttttcggcga tatttttggc ggcggacgtg gtcgtcaacg tgcggcgcgc ggtgctgatt
     4141 tacgctataa catggagctc accctcgaag aagctgtacg tggcgtgacc aaagagatcc
     4201 gcattccgac tctggaagag tgtgacgttt gccacggtag cggtgcaaaa ccaggtacac
     4261 agccgcagac ttgtccgacc tgtcatggtt ctggtcaggt gcagatgcgc cagggattct
     4321 tcgctgtaca gcagacctgt ccacactgtc agggccgcgg tacgctgatc aaagatccgt
     4381 gcaacaaatg tcatggtcat ggtcgtgttg agcgcagcaa aacgctgtcc gttaaaatcc
     4441 cggcaggggt ggacactgga gaccgcatcc gtcttgcggg cgaaggtgaa gcgggcgagc
     4501 atggcgcacc ggcaggcgat ctgtacgttc aggttcaggt taaacagcac ccgattttcg
     4561 agcgtgaagg caacaacctg tattgcgaag tcccgatcaa cttcgctatg gcggcgctgg
     4621 gtggcgaaat cgaagtaccg acccttgatg gtcgcgtcaa actgaaagtg cctggcgaaa
     4681 cccagaccgg taagctattc cgtatgcgcg gtaaaggcgt caagtctgtc cgcggtggcg
     4741 cgcagggtga tttgctgtgc cgcgttgtcg tcgaaacacc ggtaggcctg aacgaaaggc
     4801 agaaacagct gctgcaagag ctgcaagaaa gcttcggtgg cccaaccggc gagcacaaca
     4861 gcccgcgctc aaagagcttc tttgatggtg tgaagaagtt ttttgacgac ctgacccgct
     4921 aacctcgcgg agaaattcat gagtagtaaa gaacagaaaa cgcctgaggg gcaagccccg
     4981 gaagaaatta tcatggatca gcacgaagag attgaggcag ttgagccaga agcttctgct
     5041 gagcaggtgg atccgcgcga tgaaaaagtt gcgaatctcg aagctcagct ggctgaagcc
     5101 cagacccgtg aacgtgacgg cattttgcgt gtaaaagccg aaatggaaaa cctgcgtcgt
     5161 cgtactgaac tggatattga aaaagcccac aaattcgcgc tggagaaatt catcaacgaa
     5221 ttgctgccgg tgattgatag cctggatcgt gcgctggaag tggctgataa agctaacccg
     5281 gatatgtctg cgatggttga aggcattgag ctgacgctga agtcgatgct ggatgttgtg
     5341 cgtaagtttg gcgttgaagt gatcgccgaa actaacgtcc cactggaccc gaatgtgcat
     5401 caggccatcg caatggtgga atctgatgac gttgcgccag gtaacgtact gggcattatg
     5461 cagaagggtt atacgctgaa tggtcgtacg attcgtgcgg cgatggttac tgtagcgaaa
     5521 gcaaaagctt aatttctgct ttcgtaataa ttcacggccc tgcatgcaag ctcagatctg
     5581 agcttggctg ttttggcgga tgagagaaga ggtcgactct agaggatcta ctagtcatag
     5641 gcgccgatat cagatccggt accaagctta tcgatgataa gctgtcaaac atgagaatta
     5701 caacttatat cgtatggggc tgacttcagg tgctacattt gaagagataa attgcactga
     5761 aatctagaaa tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg
     5821 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
     5881 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
     5941 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
     6001 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
     6061 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     6121 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     6181 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
     6241 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     6301 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg
     6361 gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt
     6421 cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg
     6481 aagcggaata tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc
     6541 tgccacatga agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca
     6601 ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct
     6661 gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat
     6721 acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat aaatacctgt
     6781 gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc
     6841 ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca
     6901 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
     6961 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     7021 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     7081 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     7141 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tc
//
