LOCUS       62056_13410        2031 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            223..332
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     complement(421..437)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(474..492)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(507..895)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1029..1820)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(1821..1923)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 caattgatat cgcgcgcgta atacgactca ctatagggcg aattgggtac ctagggataa
       61 cagggtaata gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
      121 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
      181 ttccttggtg tatccaacgg cgtcagccgg gcggcgtcag ccgggcagga taggtgaagt
      241 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
      301 caacgggaat cctgctctgc gaggctggcc ggcgtcgact gatcaacgcg tctcgaggcc
      361 ggccagcctc gcagagcagg ggtatcgata agcttgatat cgaattcctg cagcccgggg
      421 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
      481 tgagggttaa ttgcgcgcaa ttcccatgtc agccgttaag tgttcctgtg tcactcaaaa
      541 ttgctttgag aggctctaag ggcttctcag tgcgttacat ccctggcttg ttgtccacaa
      601 ccgttaaacc ttaaaagctt taaaagcctt atatattctt ttttttctta taaaacttaa
      661 aaccttagag gctatttaag ttgctgattt atattaattt tattgttcaa acatgagagc
      721 ttagtacgtg aaacatgaga gcttagtacg ttagccatga gagcttagta cgttagccat
      781 gagggtttag ttcgttaaac atgagagctt agtacgttaa acatgagagc ttagtacgtg
      841 aaacatgaga gcttagtacg tactatcaac aggttgaact gctgatcttc agatcctcta
      901 cgccggacgc atcgtggccg gatcagatct gatatcgtcg cagaccaaaa cgatctcaag
      961 aagatcatct tattaatcag ataaaatatt tctaggcacc aataactgcc ttaaaaaaac
     1021 tgcagtcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
     1081 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
     1141 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
     1201 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
     1261 gtcacgacga gatcctcgcc gtcgggcatc cgcgccttga gcctggcgaa cagttcggct
     1321 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
     1381 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
     1441 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
     1501 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
     1561 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
     1621 agccgcgctg cctcgtcttg gagttcattc agggcaccgg acaggtcggt cttgacaaaa
     1681 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
     1741 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
     1801 aatccatctt gttcaatcat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     1861 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     1921 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     1981 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc g
//
