LOCUS       62056_13385       12986 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    75..99
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(192..444)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(487..1278)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(1312..1491)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    1630..1754
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1779..1809
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1863..2519
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2864..3166
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3210..3334)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3345..4061
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYE"
     CDS             4068..5876
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADFRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     misc_feature    6205..6229
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7560..8186
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     rep_origin      9759..9953
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10297..10438
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10624..11212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11461..12249)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcggatgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
       61 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
      121 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattgaa ttatcagctt
      181 gcatgccggt cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
      241 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
      301 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
      361 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
      421 ttattgccaa atgtttgaac gatctgcttg actctagcta gagtccgaac cccagagtcc
      481 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
      541 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
      601 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
      661 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccgtgggt
      721 cacgacgaga tcctcgccgt cgggcatccg cgccttgagc ctggcgaaca gttcggctgg
      781 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
      841 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
      901 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
      961 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     1021 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     1081 ccgcgctgcc tcgtcttgga gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     1141 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     1201 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     1261 tccatcttgt tcaatcatgc ctcgatcgag ttgagagtga atatgagact ctaattggat
     1321 accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg
     1381 atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct
     1441 cattaaactc cagaaacccg cggctcagtg gctccttcaa cgttgcggtt ctgtcagttc
     1501 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
     1561 ctccgctcat gatcgataat tcgagctctc ccatatggtc gacctgcagg cggccgcact
     1621 agtgatatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     1681 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     1741 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     1801 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     1861 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     1921 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     1981 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     2041 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     2101 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     2161 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     2221 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     2281 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     2341 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     2401 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     2461 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     2521 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     2581 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     2641 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     2701 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     2761 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     2821 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     2881 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     2941 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     3001 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     3061 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     3121 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     3181 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     3241 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     3301 acgtttctcg ttcagctttc ttgtacaaag tggtgatatc ccggatggtg agcaagggcg
     3361 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     3421 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     3481 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     3541 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     3601 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3661 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     3721 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     3781 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     3841 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     3901 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     3961 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4021 ccgccgccgg gatcactctc ggcatggacg agctgtacga acccggcatg ttacgtcctg
     4081 tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc
     4141 gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa
     4201 ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agattttcgt aattatgcgg
     4261 gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg
     4321 tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga
     4381 tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg
     4441 ggaaaagtgt acgtatcacc gtttgcgtga acaacgaact gaactggcag actatcccgc
     4501 cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc catgatttct
     4561 ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac acctgggtgg
     4621 acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct gttgactggc
     4681 aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg
     4741 caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg
     4801 gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct
     4861 acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc
     4921 acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag
     4981 gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact
     5041 cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg
     5101 gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt
     5161 tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc
     5221 agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg
     5281 tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt
     5341 tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca
     5401 atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc
     5461 tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac
     5521 tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat
     5581 acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt
     5641 atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg
     5701 gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg
     5761 gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc
     5821 aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaatgac
     5881 catggcggcc gggagggcca tgctagagtc cgcaaaaatc accagtctct ctctacaaat
     5941 ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag
     6001 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     6061 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacct
     6121 gcaggcatgc gacgtcgggc ccaagcttag cttgagcttg gatcagattg tcgtttcccg
     6181 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     6241 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     6301 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt taaagtactt
     6361 taaagtactt taaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     6421 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     6481 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     6541 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     6601 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     6661 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     6721 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     6781 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     6841 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     6901 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     6961 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     7021 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     7081 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     7141 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     7201 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     7261 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     7321 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     7381 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     7441 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     7501 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     7561 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     7621 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     7681 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     7741 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     7801 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     7861 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     7921 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     7981 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     8041 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     8101 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     8161 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     8221 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     8281 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     8341 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     8401 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     8461 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     8521 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
     8581 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     8641 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     8701 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     8761 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     8821 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     8881 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     8941 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     9001 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     9061 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     9121 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     9181 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     9241 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     9301 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     9361 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     9421 ctttttgatc gatcccggca tcggccgttt tctctaccgc gcctggcacg ccgcgccgca
     9481 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     9541 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     9601 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     9661 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     9721 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg
     9781 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     9841 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     9901 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     9961 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
    10021 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
    10081 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
    10141 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10201 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10261 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
    10321 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
    10381 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaaatacc gcatcaggcg
    10441 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    10501 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    10561 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    11041 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    11101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    11161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    11221 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    11281 ggtcatgcat gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat
    11341 aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaacg
    11401 cttgagttaa gccgcgccgc gaagcggcgt cggcttgaac gaatttctag ctagacatta
    11461 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
    11521 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
    11581 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
    11641 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
    11701 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
    11761 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
    11821 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
    11881 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
    11941 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
    12001 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
    12061 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
    12121 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
    12181 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
    12241 ttcccccatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
    12301 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
    12361 catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
    12421 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
    12481 ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgcccgaa ttgatcacag
    12541 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    12601 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    12661 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    12721 gtggcgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    12781 attgtggata aacaaattga cgcttaaaaa acttaataac acattgcgga tgttttaatg
    12841 aactgaatta cccccaaatt gaattttaaa cttgcatgcc ggtcgatctt ataaaatata
    12901 tgacacagcg cgagataatt tatcttagat tgaccgctcc atttgtttgc tatcatatat
    12961 cctttgcata ttcgcctgaa atctct
//
