LOCUS       40924_26649        3934 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pKEx4tr, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Mindrinos M, Katagiri F, Yu GL, Ausubel FM.
  TITLE     The A. thaliana disease resistance gene RPS2 encodes a protein 
            containing a nucleotide-binding site and leucine-rich repeats
  JOURNAL   Cell 78 (6), 1089-1099 (1994)
  PUBMED    7923358
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Katagiri F, Yuan F, Ausubel FM.
  TITLE     A plant cDNA expression vector carrying a modified version of the 
            cauliflower mosaic virus 35S promoter that has a reduced activity in
            bacteria
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3934)
  AUTHORS   Katagiri F, Ausubel FM.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-1998) Biological Sciences, University of Maryland 
            Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
REFERENCE   4  (bases 1 to 3934)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3934)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1994"; volume: "78"; issue: "6"; pages: "1089-1099"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (21-JAN-1998) Biological Sciences, University of Maryland Baltimore 
            County, 1000 Hilltop Circle, Baltimore, MD 21250, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1199..1245
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        1312..1330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1335..1389
                     /note="multiple cloning site; contains unique restriction
                     sites for PmeI, SalI, EcoRI, BamHI, XhoI, NotI, SacII, and 
                     SacI"
     promoter        complement(1402..1420)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      1437..1689
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1713..1729)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1737..1753)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1761..1791)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1806..1827)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2115..2703)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2877..3734)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3735..3839)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcc
      421 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
      481 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
      541 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
      601 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
      661 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
      721 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
      781 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
      841 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
      901 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
      961 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     1021 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     1081 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     1141 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aataggaaca
     1201 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtc tagccccttc
     1261 ctctatataa ggaagttaac ttcatttgga gaggacacgg gggactctag ataatacgac
     1321 tcactatagg gagagtttaa acgtcgacga attcggatcc ctcgagcggc cgccaccgcg
     1381 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcgaat ttccccgatc
     1441 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     1501 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     1561 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     1621 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     1681 tactagatcg ggaattggcc cagcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     1741 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1801 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1861 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1921 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1981 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2041 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2101 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2161 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     2341 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2401 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2461 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2521 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2581 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2641 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2701 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2761 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2821 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2881 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2941 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3001 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3061 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3121 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3181 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3241 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3301 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3361 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3421 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3481 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3541 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3601 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3661 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3721 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3781 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3841 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3901 tataaaaata ggcgtatcac gaggcccttt cgtc
//
