LOCUS       Exported                7992 bp DNA     circular SYN 28-NOV-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7992)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3133..4470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(14..889)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        916..1200
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             1244..1657
                     /codon_start=1
                     /label=Gam
                     /note="inhibitor of the host RecBCD nuclease in the lambda
                     Red system"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             1666..2448
                     /codon_start=1
                     /label=Beta
                     /note="single-stranded DNA binding recombinase in the
                     lambda Red system"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             2448..3125
                     /codon_start=1
                     /label=Exo
                     /note="5' to 3' double-stranded DNA exonuclease in the
                     lambda
                     Red system"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      4467..4711
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     CDS             complement(4837..5784)
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(5832..6054)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     promoter        6700..6728
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             6776..7963
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
       61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaag tagagttgat
      121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
      181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
      241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
      301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
      361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
      421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
      481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
      541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
      601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
      661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
      721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
      781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
      841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
      901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
      961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga
     1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc
     1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat
     1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga
     1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc
     1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt
     1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac
     1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc
     1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa
     1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc
     1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc
     1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc
     1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca
     1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa
     2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg
     2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga
     2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact
     2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt
     2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc
     2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa
     2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca
     2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc
     2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc
     2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg
     2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg
     2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga
     2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg
     2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg
     2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga
     2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc
     3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg
     3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat
     3121 ggcgatgacg cactgatgaa tcccctaatg atttttatca aaatcattaa gttaaggtag
     3181 atacacatct tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca gacaacaaaa
     3241 tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa
     3301 aacgactcaa cagcttaacg ttggcttgcc acgccttact tgactgtaaa actctcactc
     3361 ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa
     3421 tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc
     3481 atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata
     3541 tccgtgagca aaaacggctt atggtattgc gagcttcagt cgcactacac ggtcgttctg
     3601 ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc
     3661 aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg
     3721 atgctggctt taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa
     3781 gtcgagtaag aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca
     3841 gcaacttaca tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta
     3901 aaagcaatcc aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa
     3961 atcagcgctc gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg
     4021 caaaggagcc atggattcta gcaactaact tacctgttga aattcgaaca cccaaacaac
     4081 ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc
     4141 ctgcctacgg actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca
     4201 tgctgctaat cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga
     4261 aacaaggttg ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa
     4321 cagttcgctt aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact
     4381 tactcgtggc tgcaacccta ctagctcaaa atttattcac acatggttac gctttgggga
     4441 aattatgagg ggatctctca ggatgacgca tcctcacgat aatatccggg taggcgcaat
     4501 cactttcgtc tactccgtta caaagcgagg ctgggtattt cccggccttt ctgttatccg
     4561 aaatccactg aaagcacagc ggctggctga ggagataaat aataaacgag gggctgtatg
     4621 cacaaagcat cttctgttga gttaagaacg agtatcgaga tggcacatag ccttgctcaa
     4681 attggaatca ggtttgtgcc aataccagta gaaacagacg aagaatccat gggtatggac
     4741 agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat
     4801 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat
     4861 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata
     4921 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt
     4981 taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt
     5041 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc
     5101 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc
     5161 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca
     5221 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc
     5281 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa
     5341 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata
     5401 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc
     5461 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac
     5521 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac
     5581 accataagca ttttccctac tgatgttcat catctgaacg tattggttat aagtgaacga
     5641 taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca
     5701 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc
     5761 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata
     5821 ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat
     5881 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa
     5941 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat
     6001 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact
     6061 ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa
     6121 cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcggttg cggccgcaat
     6181 cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt
     6241 tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac
     6301 tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc
     6361 acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct
     6421 gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag
     6481 gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacgg ggtctgacgc
     6541 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6601 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6661 aacttggtct gacagtttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa
     6721 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
     6781 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     6841 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     6901 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     6961 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     7021 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg
     7081 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     7141 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     7201 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     7261 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     7321 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     7381 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     7441 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     7501 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     7561 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     7621 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     7681 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     7741 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     7801 tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc
     7861 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     7921 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgacaca tttccccgaa
     7981 aagtgccacc tg
//
