LOCUS       62056_13345       12086 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(14..889)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        916..1200
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             1244..1657
                     /codon_start=1
                     /label=Gam
                     /note="inhibitor of the host RecBCD nuclease in the lambda 
                     Red system"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             1666..2448
                     /codon_start=1
                     /label=Beta
                     /note="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             2448..3125
                     /codon_start=1
                     /label=Exo
                     /note="5' to 3' double-stranded DNA exonuclease in the 
                     lambda
                     Red system"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      3129..3373
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     CDS             complement(3393..4811)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        4813..4868
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             4904..9007
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      9034..9105
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      9121..9148
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(9256..10203)
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(10251..10473)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(11098..11955)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11956..12060)
                     /label=AmpR promoter
ORIGIN
        1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
       61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
      121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
      181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
      241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
      301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
      361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
      421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
      481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
      541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
      601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
      661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
      721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
      781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
      841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
      901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
      961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga
     1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc
     1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat
     1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga
     1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc
     1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt
     1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac
     1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc
     1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa
     1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc
     1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc
     1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc
     1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca
     1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa
     2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg
     2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga
     2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact
     2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt
     2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc
     2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa
     2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca
     2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc
     2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc
     2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg
     2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg
     2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga
     2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg
     2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg
     2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga
     2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc
     3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg
     3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat
     3121 ggcgatgacg catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt
     3181 tacaaagcga ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca
     3241 gcggctggct gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt
     3301 gagttaagaa cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg
     3361 ccaataccag tagaaacaga cgaagaatct tatttgttaa ctgttaattg tccttgttca
     3421 aggatgctgt ctttgacaac agatgttttc ttgcctttga tgttcagcag gaagctcggc
     3481 gcaaacgttg attgtttgtc tgcgtagaat cctctgtttg tcatatagct tgtaatcacg
     3541 acattgtttc ctttcgcttg aggtacagcg aagtgtgagt aagtaaaggt tacatcgtta
     3601 ggatcaagat ccatttttaa cacaaggcca gttttgttca gcggcttgta tgggccagtt
     3661 aaagaattag aaacataacc aagcatgtaa atatcgttag acgtaatgcc gtcaatcgtc
     3721 atttttgatc cgcgggagtc agtgaacagg taccatttgc cgttcatttt aaagacgttc
     3781 gcgcgttcaa tttcatctgt tactgtgtta gatgcaatca gcggtttcat cacttttttc
     3841 agtgtgtaat catcgtttag ctcaatcata ccgagagcgc cgtttgctaa ctcagccgtg
     3901 cgttttttat cgctttgcag aagtttttga ctttcttgac ggaagaatga tgtgcttttg
     3961 ccatagtatg ctttgttaaa taaagattct tcgccttggt agccatcttc agttccagtg
     4021 tttgcttcaa atactaagta tttgtggcct ttatcttcta cgtagtgagg atctctcagc
     4081 gtatggttgt cgcctgagct gtagttgcct tcatcgatga actgctgtac attttgatac
     4141 gtttttccgt caccgtcaaa gattgattta taatcctcta caccgttgat gttcaaagag
     4201 ctgtctgatg ctgatacgtt aacttgtgca gttgtcagtg tttgtttgcc gtaatgttta
     4261 ccggagaaat cagtgtagaa taaacggatt tttccgtcag atgtaaatgt ggctgaacct
     4321 gaccattctt gtgtttggtc ttttaggata gaatcatttg catcgaattt gtcgctgtct
     4381 ttaaagacgc ggccagcgtt tttccagctg tcaatagaag tttcgccgac tttttgatag
     4441 aacatgtaaa tcgatgtgtc atccgcattt ttaggatctc cggctaatgc aaagacgatg
     4501 tggtagccgt gatagtttgc gacagtgccg tcagcgtttt gtaatggcca gctgtcccaa
     4561 acgtccaggc cttttgcaga agagatattt ttaattgtgg acgaatcaaa ttcagaaact
     4621 tgatattttt catttttttg ctgttcaggg atttgcagca tatcatggcg tgtaatatgg
     4681 gaaatgccgt atgtttcctt atatggcttt tggttcgttt ctttcgcaaa cgcttgagtt
     4741 gcgcctcctg ccagcagtgc ggtagtaaag gttaatactg ttgcttgttt tgcaaacttt
     4801 ttgatgttca tcgttgacac tctatcgttg atagagttat tttaccactc cctatcagtg
     4861 atagagaaaa gaattcaaaa gatctaaaga ggagaaagga tctatggata agaaatactc
     4921 aataggctta gatatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa
     4981 ggttccgtct aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa
     5041 tcttataggg gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg
     5101 gacagctcgt agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt
     5161 ttcaaatgag atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt
     5221 ggtggaagaa gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt
     5281 tgcttatcat gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac
     5341 tgataaagcg gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg
     5401 tcattttttg attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat
     5461 ccagttggta caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt
     5521 agatgctaaa gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat
     5581 tgctcagctc cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt
     5641 gggtttgacc cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct
     5701 ttcaaaagat acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata
     5761 tgctgatttg tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct
     5821 aagagtaaat actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga
     5881 tgaacatcat caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa
     5941 gtataaagaa atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg
     6001 agctagccaa gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac
     6061 tgaggaatta ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga
     6121 caacggctct attccccatc aaattcactt gggtgagctg catgctattt tgagaagaca
     6181 agaagacttt tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt
     6241 tcgaattcct tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac
     6301 tcggaagtct gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc
     6361 ttcagctcaa tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa
     6421 agtactacca aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa
     6481 ggtcaaatat gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa
     6541 agccattgtt gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga
     6601 agattatttc aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag
     6661 atttaatgct tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt
     6721 tttggataat gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt
     6781 tgaagatagg gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa
     6841 ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt
     6901 gattaatggt attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga
     6961 tggttttgcc aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga
     7021 agacattcaa aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa
     7081 tttagctggt agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga
     7141 attggtcaaa gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga
     7201 aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga
     7261 aggtatcaaa gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt
     7321 gcaaaatgaa aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca
     7381 agaattagat attaatcgtt taagtgatta tgatgtcgat cacattgttc cacaaagttt
     7441 ccttaaagac gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa
     7501 atcggataac gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact
     7561 tctaaacgcc aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg
     7621 aggtttgagt gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca
     7681 aatcactaag catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa
     7741 tgataaactt attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt
     7801 ccgaaaagat ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga
     7861 tgcgtatcta aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc
     7921 ggagtttgtc tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga
     7981 gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt
     8041 caaaacagaa attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa
     8101 tggggaaact ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt
     8161 attgtccatg ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc
     8221 caaggagtca attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg
     8281 ggatccaaaa aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt
     8341 tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat
     8401 cacaattatg gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg
     8461 atataaggaa gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt
     8521 agaaaacggt cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct
     8581 ggctctgcca agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa
     8641 gggtagtcca gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt
     8701 agatgagatt attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa
     8761 tttagataaa gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc
     8821 agaaaatatt attcatttat ttacgttgac gaatcttgga gctcccgctg cttttaaata
     8881 ttttgataca acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac
     8941 tcttatccat caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg
     9001 aggtgactaa ctcgagtaag gatctccagg catcaaataa aacgaaaggc tcagtcgaaa
     9061 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     9121 ggctcacctt cgggtgggcc tttctgcgca ggtatggaca gttttccctt tgatatgtaa
     9181 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     9241 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     9301 tttttgcgtg agccatgaga acgaaccatt gagatcatac ttactttgca tgtcactcaa
     9361 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     9421 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     9481 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     9541 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     9601 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     9661 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     9721 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     9781 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     9841 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     9901 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     9961 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
    10021 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
    10081 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
    10141 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
    10201 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
    10261 atgataatta ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt
    10321 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
    10381 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
    10441 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
    10501 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
    10561 cttaagtagc accctcgcaa gctcggttgc ggccgcaatc gggcaaatcg ctgaatattc
    10621 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct
    10681 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt
    10741 catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt
    10801 atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct
    10861 gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc
    10921 acccagtaag gcagcggtat catcaacggg gtctgacgct cagtggaacg aaaactcacg
    10981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    11041 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    11101 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    11161 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    11221 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    11281 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    11341 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    11401 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    11461 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    11521 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    11581 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11641 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11701 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11761 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11821 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11881 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11941 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    12001 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    12061 cacatttccc cgaaaagtgc cacctg
//
