LOCUS       Exported                3470 bp DNA     circular SYN 31-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3470)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3470)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2069..2105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(51..98)
                     /label=FLP recombinase from the Saccharomyces
                     cerevisi
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(114..905)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(1273..1306)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     terminator      1386..1632
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     rep_origin      complement(1693..2113)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2186..3043)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3044..3148)
                     /label=AmpR promoter
     terminator      3238..3324
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3416..3443
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta
       61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctta ttagaagaac
      121 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
      181 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
      241 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
      301 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
      361 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
      421 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
      481 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
      541 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
      601 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
      661 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
      721 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
      781 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
      841 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
      901 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
      961 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     1021 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
     1081 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
     1141 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
     1201 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgagctt
     1261 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcgaac tgcaggtcga
     1321 cggatccccg gaattaattc tcatgtttga cagcttatca ctgatcagtg aattaatggc
     1381 gatgacgcat cctcacgata atatccgggt aggcgcaatc actttcgtct ctactccgtt
     1441 acaaagcgag gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag
     1501 cggctggctg aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg
     1561 agttaagaac gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc
     1621 caataccagt agaaacagac gaagaagcta gctttgcact ggattgcgag gctttgccat
     1681 ggctaattcc catgtcagcc gttaagtgtt cctgtgtcac tcaaaattgc tttgagaggc
     1741 tctaagggct tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa
     1801 aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta
     1861 tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac
     1921 atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg
     1981 ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt
     2041 agtacgtact atcaacaggt tgaactgctg atcttcagat cctctacgcc ggacgcatcg
     2101 tggccggatc ttgcggccgc aaaaattaaa aatgaagttt taaatcaatc taaagtatat
     2161 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2221 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2281 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2341 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2401 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2461 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2521 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2581 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2641 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2701 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2761 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2821 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2881 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2941 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3001 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3061 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3121 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcatcga
     3181 tggcccccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
     3241 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     3301 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
     3361 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
     3421 gccatcctga cggatggcct ttttgcgtgg ccagtgccaa gcttgcatgc
//
