LOCUS       40924_26576       12615 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gal4/UAS cloning vector pKC26-FB1.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12615)
  AUTHORS   Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, 
            Salecker I.
  TITLE     Flybow: genetic multicolor cell labeling for neural circuit analysis
            in Drosophila melanogaster
  JOURNAL   Nat. Methods (2011) In press
  PUBMED    21297619
REFERENCE   2  (bases 1 to 12615)
  AUTHORS   Hadjieconomou D, Rotkopf S, Alexandre C, Dickson BJ, Salecker I.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2011) Division of Molecular Neurobiology, MRC 
            National Institute for Medical Research, The Ridgeway, London, 
            England NW7 1AA, UK
REFERENCE   3  (bases 1 to 12615)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12615)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods (2011) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2011) Division of Molecular Neurobiology, MRC National 
            Institute for Medical Research, The Ridgeway, London, England NW7 
            1AA, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..866
                     /label=5' mini-white fragment
                     /note="5' mini-white fragment"
     protein_bind    884..917
                     /label=attB
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et
                     al., 2000)"
     primer_bind     complement(935..951)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1425..1529
                     /label=AmpR promoter
     CDS             1530..2387
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2561..3149
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3437..3458
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3473..3503
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3511..3527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3535..3551
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3598..3692
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3716..3810
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3829..4067
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    4081..4110
                     /label=altered multiple cloning site
                     /note="altered multiple cloning site"
     misc_recomb     4111..4144
                     /label=mFRT71
                     /note="mFRT71"
     misc_feature    4151..4816
                     /note="membrane anchor; Region: Cd8a"
     CDS             4151..4816
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             4823..5539
                     /label=EGFP
                     /note="enhanced GFP"
     intron          5625..5690
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5820..5840
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    6265..6399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6423..6849
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     regulatory      complement(6424..6852)
                     /label=hsp70Ab polyA signal
                     /note="hsp70Ab polyA signal"
                     /regulatory_class="polyA_signal_sequence"
     CDS             complement(6862..7578)
                     /label=Citrine
                     /note="enhanced variant of YFP (Heikal et al., 2001)"
     misc_feature    complement(7591..7638)
                     /label=Region: Lyn kinase
                     /note="Region: Lyn kinase"
     misc_recomb     complement(7645..7678)
                     /label=mFRT71
                     /note="mFRT71"
     misc_recomb     7691..7724
                     /label=mFRT71
                     /note="mFRT71"
     CDS             7731..8396
                     /label=mCD8
                     /note="mouse lymphocyte antigen CD8 alpha chain"
     CDS             8403..9110
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     intron          9196..9261
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9391..9411
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    9836..9970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(9978..10404)
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     misc_feature    complement(10424..10468)
                     /label=Region: 1xV5 tag
                     /note="Region: 1xV5 tag"
     CDS             complement(10424..10465)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(10475..11191)
                     /label=mCerulean
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     CDS             complement(11198..11863)
                     /label=mCD8
                     /note="mouse lymphocyte antigen CD8 alpha chain"
     misc_recomb     11870..11903
                     /label=mFRT71
                     /note="mFRT71"
     intron          11993..12058
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12188..12208
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    12480..12614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gatcccccgt tattctctat tcgttttgtg actctccctc tctgtactat tgctctctca
       61 ctctgtcgca cagtaaacgg cacactattc tcgttgcttc gagagagcgc gcctcgaatg
      121 ttcgcgaaaa gagcgccgga gtataaatag aggcgcttcg tcgacggagc gtcaattcaa
      181 ttcaaacaag caaagtgaac acatcgcgaa gcgtaagctg agcaaacaaa caagcgcagc
      241 tgaacaagct aaacaatctg caataaagtg caagttaaag tgaatcaatt aaaagtaacc
      301 aacaaccaag taattaaact aaaaactgca actactgaaa tcaaccaaga agtcattatt
      361 gaagacaaga agagaactct gaataggtcg atagcgtcaa tgtccgcctt cagttgcact
      421 ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg
      481 ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg
      541 attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg
      601 atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg
      661 tgagtagtac atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac
      721 atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caatcgcaca
      781 aacaaatagt gacacgaaac agattattct ggtagctgtg ctcgctatat aagacaattt
      841 ttaagatcat atcatgatcc tctagcacta gtccgcggtg cgggtgccag ggcgtgccct
      901 tgggctcccc gggcgcgtac tccacaagct tggcactggc cgtcgtttta caacgtcgtg
      961 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1021 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1081 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1141 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     1201 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     1261 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1321 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1381 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1441 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1501 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1561 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1621 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1681 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1741 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1801 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1861 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1921 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1981 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2041 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2101 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2161 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2221 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2281 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2341 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2401 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2461 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2701 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3181 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3241 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3301 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3361 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3421 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     3481 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     3541 acagctatga ccatgattac gaattgtacc cggggatccg cttgcatgcc tgcaggtcgg
     3601 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3661 actgtcctcc gagcggagta ctgtcctccg agcggaagct tgcatgcctg caggtcggag
     3721 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     3781 tgtcctccga gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa
     3841 tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc
     3901 taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa
     3961 gtgcaagtta aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta
     4021 ctgaaatctg ccaagaagta attattgaat acaagaagag aactctgaat agggaattgg
     4081 gaattcgcta gcagatctgg taccctcgag gaagtttcta tactttctag agaatagaaa
     4141 cttcactagt atggcctcac cgttgacccg ctttctgtcg ctgaacctgc tgctgctggg
     4201 tgagtcgatt atcctgggga gtggagaagc taagccacag gcacccgaac tccgaatctt
     4261 tccaaagaaa atggacgccg aacttggtca gaaggtggac ctggtatgtg aagtgttggg
     4321 gtccgtttcg caaggatgct cttggctctt ccagaactcc agctccaaac tcccccagcc
     4381 caccttcgtt gtctatatgg cttcatccca caacaagata acgtgggacg agaagctgaa
     4441 ttcgtcgaaa ctgttttctg ccatgaggga cacgaataat aagtacgttc tcaccctgaa
     4501 caagttcagc aaggaaaacg aaggctacta tttctgctca gtcatcagca actcggtgat
     4561 gtacttcagt tctgtcgtgc cagtccttca gaaagtgaac tctactacta ccaagccagt
     4621 gctgcgaact ccctcacctg tgcaccctac cgggacatct cagccccaga gaccagaaga
     4681 ttgtcggccc cgtggctcag tgaaggggac cggattggac ttcgcctgtg atatttacat
     4741 ctgggcaccc ttggccggaa tctgcgtggc ccttctgctg tccttgatca tcactctcat
     4801 ctgctaccac agccgcggat ccatggtgag caagggcgag gagctgttca ccggggtggt
     4861 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4921 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     4981 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     5041 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5101 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5161 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5221 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5281 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5341 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5401 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     5461 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5521 catggacgag ctgtacaagt aacctaggga tctttgtgaa ggaaccttac ttctgtggtg
     5581 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     5641 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     5701 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     5761 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     5821 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     5881 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     5941 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     6001 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     6061 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     6121 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     6181 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     6241 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     6301 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6361 ttgtccaaac tcatcaatgt atcttatcat gtctggatcg cggccgcaga gctctgcggc
     6421 cgctcctgac cgtccatcgc aataaaatga gccattatga gatcgaaagg gtctacgaaa
     6481 atccgttcgg aaaaaatcag aaaatcatca aagccgaata taattaaaat gtattactag
     6541 ctaaagaaat catcactaat atagaatgta gaatgaacca tgtatataga tactaatgta
     6601 tcgtaagact ttcaaaagtc tacaagacat taaatgacaa gttgacttta aatttcaaat
     6661 aaataattta ttttttctat aagcaataac atttttgcta aattaagact tggtaattag
     6721 gtaatactat tgttgttcta tggaatattc gatcgaaaca ttcttatcag tctcaaaaac
     6781 ttaaaacaaa cttataatat aacccatatg ttataaccca ttgatgaaca aaaattagac
     6841 tctttggcct tacctaggtt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
     6901 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     6961 ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     7021 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     7081 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     7141 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     7201 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     7261 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     7321 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcaca tcaggccgta
     7381 gccgaaggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     7441 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     7501 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     7561 ctcgcccttg ctcaccatgg atcctgctgc ctcgtcgtcg ttcaggttgt ccttgcgctt
     7621 gctcttgatg cagcccatac tagtgaagtt tctattctct agaaagtata gaaacttcgg
     7681 taccctcgag gaagtttcta tactttctag agaatagaaa cttcactagt atggcctcac
     7741 cgttgacccg ctttctgtcg ctgaacctgc tgctgctggg tgagtcgatt atcctgggga
     7801 gtggagaagc taagccacag gcacccgaac tccgaatctt tccaaagaaa atggacgccg
     7861 aacttggtca gaaggtggac ctggtatgtg aagtgttggg gtccgtttcg caaggatgct
     7921 cttggctctt ccagaactcc agctccaaac tcccccagcc caccttcgtt gtctatatgg
     7981 cttcatccca caacaagata acgtgggacg agaagctgaa ttcgtcgaaa ctgttttctg
     8041 ccatgaggga cacgaataat aagtacgttc tcaccctgaa caagttcagc aaggaaaacg
     8101 aaggctacta tttctgctca gtcatcagca actcggtgat gtacttcagt tctgtcgtgc
     8161 cagtccttca gaaagtgaac tctactacta ccaagccagt gctgcgaact ccctcacctg
     8221 tgcaccctac cgggacatct cagccccaga gaccagaaga ttgtcggccc cgtggctcag
     8281 tgaaggggac cggattggac ttcgcctgtg atatttacat ctgggcaccc ttggccggaa
     8341 tctgcgtggc ccttctgctg tccttgatca tcactctcat ctgctaccac agccgcggat
     8401 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     8461 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     8521 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     8581 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     8641 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     8701 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     8761 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     8821 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     8881 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     8941 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     9001 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     9061 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taacctaggg
     9121 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     9181 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     9241 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     9301 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     9361 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     9421 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     9481 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     9541 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     9601 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
     9661 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
     9721 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     9781 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     9841 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9901 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9961 tgtctggatc gcggccgctc ctgaccgtcc atcgcaataa aatgagccat tatgagatcg
    10021 aaagggtcta cgaaaatccg ttcggaaaaa atcagaaaat catcaaagcc gaatataatt
    10081 aaaatgtatt actagctaaa gaaatcatca ctaatataga atgtagaatg aaccatgtat
    10141 atagatacta atgtatcgta agactttcaa aagtctacaa gacattaaat gacaagttga
    10201 ctttaaattt caaataaata atttattttt tctataagca ataacatttt tgctaaatta
    10261 agacttggta attaggtaat actattgttg ttctatggaa tattcgatcg aaacattctt
    10321 atcagtctca aaaacttaaa acaaacttat aatataaccc atatgttata acccattgat
    10381 gaacaaaaat tagactcttt ggccttacct aggttaaacc tgcagtagaa tcgagaccga
    10441 ggagagggtt agggataggc ttaccactag gggacttgta cagctcgtcc atgccgagag
    10501 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
    10561 ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
    10621 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
    10681 tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga
    10741 cgttgtcgct gatggcgttg tactccagct tgtgccccag gatgttgccg tcctccttga
    10801 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
    10861 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
    10921 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
    10981 actgcacgcc ccaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
    11041 tggtgcagat gaacttcagg ttcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
    11101 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
    11161 cggtgaacag ctcctcgccc ttgctcacca tggatccgcg gctgtggtag cagatgagag
    11221 tgatgatcaa ggacagcaga agggccacgc agattccggc caagggtgcc cagatgtaaa
    11281 tatcacaggc gaagtccaat ccggtcccct tcactgagcc acggggccga caatcttctg
    11341 gtctctgggg ctgagatgtc ccggtagggt gcacaggtga gggagttcgc agcactggct
    11401 tggtagtagt agagttcact ttctgaagga ctggcacgac agaactgaag tacatcaccg
    11461 agttgctgat gactgagcag aaatagtagc cttcgttttc cttgctgaac ttgttcaggg
    11521 tgagaacgta cttattattc gtgtccctca tggcagaaaa cagtttcgac gaattcagct
    11581 tctcgtccca cgttatcttg ttgtgggatg aagccatata gacaacgaag gtgggctggg
    11641 ggagtttgga gctggagttc tggaagagcc aagagcatcc ttgcgaaacg gaccccaaca
    11701 cttcacatac caggtccacc ttctgaccaa gttcggcgtc cattttcttt ggaaagattc
    11761 ggagttcggg tgcctgtggc ttagcttctc cactccccag gataatcgac tcacccagca
    11821 gcagcaggtt cagcgacaga aagcgggtca acggtgaggc catactagtg aagtttctat
    11881 tctctagaaa gtatagaaac ttcctcgagt ctagaggatc tttgtgaagg aaccttactt
    11941 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
    12001 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
    12061 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
    12121 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
    12181 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
    12241 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
    12301 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt tgatgtatag
    12361 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
    12421 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    12481 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    12541 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    12601 atcatgtctg gatcg
//
