LOCUS       40924_26571        9041 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gal4/UAS cloning vector pKC26-FB1.0, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, 
            Salecker I.
  TITLE     Flybow: genetic multicolor cell labeling for neural circuit analysis
            in Drosophila melanogaster
  JOURNAL   Nat. Methods (2011) In press
  PUBMED    21297619
REFERENCE   2  (bases 1 to 9041)
  AUTHORS   Hadjieconomou D, Rotkopf S, Alexandre C, Dickson BJ, Salecker I.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2011) Division of Molecular Neurobiology, MRC 
            National Institute for Medical Research, The Ridgeway, London, 
            England NW7 1AA, UK
REFERENCE   3  (bases 1 to 9041)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9041)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods (2011) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2011) Division of Molecular Neurobiology, MRC National 
            Institute for Medical Research, The Ridgeway, London, England NW7 
            1AA, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..866
                     /label=5' mini-white fragment
                     /note="5' mini-white fragment"
     protein_bind    884..917
                     /label=attB
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et
                     al., 2000)"
     primer_bind     complement(935..951)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1425..1529
                     /label=AmpR promoter
     CDS             1530..2387
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2561..3149
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3437..3458
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3473..3503
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3511..3527
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3535..3551
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3598..3692
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3716..3810
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3829..4067
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    4081..4116
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_recomb     4117..4150
                     /label=mFRT71
                     /note="mFRT71"
     CDS             4157..4822
                     /codon_start=1
                     /label=mCD8
                     /note="mouse lymphocyte antigen CD8 alpha chain"
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             4829..5536
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     intron          5622..5687
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5817..5837
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    6262..6396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(6404..6830)
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     misc_feature    complement(6850..6894)
                     /label=Region: 1xV5 tag
                     /note="Region: 1xV5 tag"
     CDS             complement(6850..6891)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(6901..7617)
                     /codon_start=1
                     /label=mCerulean
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLNL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(7624..8289)
                     /codon_start=1
                     /label=mCD8
                     /note="mouse lymphocyte antigen CD8 alpha chain"
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     misc_recomb     complement(8296..8329)
                     /label=mFRT71
                     /note="mFRT71"
     intron          8419..8484
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8614..8634
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    8906..9040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gatcccccgt tattctctat tcgttttgtg actctccctc tctgtactat tgctctctca
       61 ctctgtcgca cagtaaacgg cacactattc tcgttgcttc gagagagcgc gcctcgaatg
      121 ttcgcgaaaa gagcgccgga gtataaatag aggcgcttcg tcgacggagc gtcaattcaa
      181 ttcaaacaag caaagtgaac acatcgcgaa gcgtaagctg agcaaacaaa caagcgcagc
      241 tgaacaagct aaacaatctg caataaagtg caagttaaag tgaatcaatt aaaagtaacc
      301 aacaaccaag taattaaact aaaaactgca actactgaaa tcaaccaaga agtcattatt
      361 gaagacaaga agagaactct gaataggtcg atagcgtcaa tgtccgcctt cagttgcact
      421 ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg
      481 ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg
      541 attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg
      601 atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg
      661 tgagtagtac atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac
      721 atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caatcgcaca
      781 aacaaatagt gacacgaaac agattattct ggtagctgtg ctcgctatat aagacaattt
      841 ttaagatcat atcatgatcc tctagcacta gtccgcggtg cgggtgccag ggcgtgccct
      901 tgggctcccc gggcgcgtac tccacaagct tggcactggc cgtcgtttta caacgtcgtg
      961 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1021 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1081 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1141 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     1201 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     1261 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1321 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1381 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1441 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1501 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1561 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1621 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1681 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1741 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1801 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1861 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1921 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1981 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2041 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2101 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2161 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2221 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2281 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2341 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2401 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2461 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2701 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3181 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3241 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3301 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3361 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3421 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     3481 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     3541 acagctatga ccatgattac gaattgtacc cggggatccg cttgcatgcc tgcaggtcgg
     3601 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3661 actgtcctcc gagcggagta ctgtcctccg agcggaagct tgcatgcctg caggtcggag
     3721 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     3781 tgtcctccga gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa
     3841 tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc
     3901 taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa
     3961 gtgcaagtta aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta
     4021 ctgaaatctg ccaagaagta attattgaat acaagaagag aactctgaat agggaattgg
     4081 gaattcgcta gcagatctga gctcggtacc ctcgaggaag tttctatact ttctagagaa
     4141 tagaaacttc actagtatgg cctcaccgtt gacccgcttt ctgtcgctga acctgctgct
     4201 gctgggtgag tcgattatcc tggggagtgg agaagctaag ccacaggcac ccgaactccg
     4261 aatctttcca aagaaaatgg acgccgaact tggtcagaag gtggacctgg tatgtgaagt
     4321 gttggggtcc gtttcgcaag gatgctcttg gctcttccag aactccagct ccaaactccc
     4381 ccagcccacc ttcgttgtct atatggcttc atcccacaac aagataacgt gggacgagaa
     4441 gctgaattcg tcgaaactgt tttctgccat gagggacacg aataataagt acgttctcac
     4501 cctgaacaag ttcagcaagg aaaacgaagg ctactatttc tgctcagtca tcagcaactc
     4561 ggtgatgtac ttcagttctg tcgtgccagt ccttcagaaa gtgaactcta ctactaccaa
     4621 gccagtgctg cgaactccct cacctgtgca ccctaccggg acatctcagc cccagagacc
     4681 agaagattgt cggccccgtg gctcagtgaa ggggaccgga ttggacttcg cctgtgatat
     4741 ttacatctgg gcacccttgg ccggaatctg cgtggccctt ctgctgtcct tgatcatcac
     4801 tctcatctgc taccacagcc gcggatccat ggtgagcaag ggcgaggagg ataacatggc
     4861 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     4921 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     4981 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     5041 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     5101 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     5161 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     5221 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     5281 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     5341 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     5401 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     5461 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     5521 ggacgagctg tacaagtaac ctagggatct ttgtgaagga accttacttc tgtggtgtga
     5581 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     5641 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     5701 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     5761 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     5821 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     5881 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     5941 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     6001 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     6061 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     6121 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     6181 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     6241 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     6301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6361 tccaaactca tcaatgtatc ttatcatgtc tggatcgcgg ccgctcctga ccgtccatcg
     6421 caataaaatg agccattatg agatcgaaag ggtctacgaa aatccgttcg gaaaaaatca
     6481 gaaaatcatc aaagccgaat ataattaaaa tgtattacta gctaaagaaa tcatcactaa
     6541 tatagaatgt agaatgaacc atgtatatag atactaatgt atcgtaagac tttcaaaagt
     6601 ctacaagaca ttaaatgaca agttgacttt aaatttcaaa taaataattt attttttcta
     6661 taagcaataa catttttgct aaattaagac ttggtaatta ggtaatacta ttgttgttct
     6721 atggaatatt cgatcgaaac attcttatca gtctcaaaaa cttaaaacaa acttataata
     6781 taacccatat gttataaccc attgatgaac aaaaattaga ctctttggcc ttacctaggt
     6841 taaacctgca gtagaatcga gaccgaggag agggttaggg ataggcttac cactagggga
     6901 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     6961 catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc tcaggtagtg
     7021 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     7081 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttggcct tgatgccgtt
     7141 cttctgcttg tcggcggtga tatagacgtt gtcgctgatg gcgttgtact ccagcttgtg
     7201 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     7261 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     7321 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     7381 catgtggtcg gggtagcggg cgaagcactg cacgccccag gtcagggtgg tcacgagggt
     7441 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcaggttca gcttgccgta
     7501 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     7561 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatgga
     7621 tccgcggctg tggtagcaga tgagagtgat gatcaaggac agcagaaggg ccacgcagat
     7681 tccggccaag ggtgcccaga tgtaaatatc acaggcgaag tccaatccgg tccccttcac
     7741 tgagccacgg ggccgacaat cttctggtct ctggggctga gatgtcccgg tagggtgcac
     7801 aggtgaggga gttcgcagca ctggcttggt agtagtagag ttcactttct gaaggactgg
     7861 cacgacagaa ctgaagtaca tcaccgagtt gctgatgact gagcagaaat agtagccttc
     7921 gttttccttg ctgaacttgt tcagggtgag aacgtactta ttattcgtgt ccctcatggc
     7981 agaaaacagt ttcgacgaat tcagcttctc gtcccacgtt atcttgttgt gggatgaagc
     8041 catatagaca acgaaggtgg gctgggggag tttggagctg gagttctgga agagccaaga
     8101 gcatccttgc gaaacggacc ccaacacttc acataccagg tccaccttct gaccaagttc
     8161 ggcgtccatt ttctttggaa agattcggag ttcgggtgcc tgtggcttag cttctccact
     8221 ccccaggata atcgactcac ccagcagcag caggttcagc gacagaaagc gggtcaacgg
     8281 tgaggccata ctagtgaagt ttctattctc tagaaagtat agaaacttcc tcgagtctag
     8341 aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     8401 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     8461 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     8521 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     8581 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     8641 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     8701 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     8761 attatggaaa aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     8821 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     8881 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     8941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9001 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc g
//
