LOCUS       Exported                6708 bp DNA     circular SYN 15-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6708)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          6162..6297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(170..508)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSISRAAADPLESTCSPSLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRINSPMSSTSGIG
                     SAADAKCR"
     primer_bind     424..440
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(509..525)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(533..549)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(557..587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(602..623)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(911..1499)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1703..2503
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(3442..4884)
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            6051..6160
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             6193..6564
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 gatctacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
       61 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
      121 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
      181 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt atgcggtgtg
      241 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
      301 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      361 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      421 gttgtaaaac gacggccagt gccaagcttg ggctgcaggt cgactctaga ggatccgcgg
      481 ccgcgcgcga tatcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
      541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      601 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      661 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      721 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      781 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      841 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      901 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      961 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1321 agtggtggcc taactacggc tacactagaa gaacagtgtt tggtatctgc gctctgctga
     1381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg gttcatgtgc
     1621 agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc aacagtgccg
     1681 ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga
     1741 atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc ccggcggtgt
     1801 gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc cacttggact
     1861 gatcgaggcc ctgcgtgctg cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc
     1921 aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg accttggagt
     1981 tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt
     2041 tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc tgccacctca
     2101 ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg
     2161 cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa aggttcccta
     2221 tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg tcgattatct
     2281 cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca aggagaagag
     2341 ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat
     2401 tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc atccgccaga
     2461 ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc atgagcggag
     2521 aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac gtggagcgga
     2581 tcggggattg tctttcttca gctcgctgat gatatgctga cgctcaatgc cgtttggcct
     2641 ccgactaacg aaaatcccgc atttggacgg ctgatccgat tggcacggcg gacggcgaat
     2701 ggcggagcag acgctcgtcc gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     2761 gtatcgatgt cgaggttcct caggggagcc accccagaga agccctcgga gctgagcgga
     2821 gctatttcca aagccatgcc agctagagac agtgcacacc gccaagcatc tgcaaaaccc
     2881 tcgcctggag ggaaagtgca atgtacgtac gctggtttcc ctccagaaag ggattctgcg
     2941 gcttctcaac tttggaagaa gaggcggaac gaactgctgt ttcagcctac tcatgtgagg
     3001 aggctggtcc tttaccctga tgcccgaggg catcagggta aaggaccagc ctcgccaact
     3061 aggagcgcta ccaaggcgaa cacaacccag gacttggtag aacccttcgc cggagaagtt
     3121 cagactcatc ggcatgagca cccctcgccg cgcactcggc accggtccgt cgaccaccac
     3181 caggacgtcg ttgtcgacgt cggccccgcg gctcctgccc gccgaacgcg tcgtcgtcga
     3241 cggcctggtg ctcatcgacg agcacccgga gccaggtgaa aagcgccggc ggacgctcgg
     3301 actgggcgcg ggattccagc agtaacccag gtccgccggc cacctcacgg caggcagacc
     3361 ctcggctttc gcgccgggac cgccatgaga ccgccacccg gatgtccggg gtggcggtct
     3421 catggcggtc cctcagcggc cctacgcgac cgctgtgtcg acgcggaggc agtctccggg
     3481 gtgctggccc cagggctgga gtaccggggc gagcttgccc ttgcagcggc ggcacacggg
     3541 cgacgcggcg ccgggcgggg gagtcttgac gtcgacggtg accggcttcg gcttcaggcg
     3601 cttccgaagg ctgatcgtcg ggcggatcgc ctccttcttc ggctgacgca ctcggtcgag
     3661 cgaagcggcc agctcctccg tgcgggcctc gttggccttc cggcgcgcct cgcggaaagc
     3721 agcttcctcc tcggacatga cctcgaacct catctggtca gcgtcaaggt cgcccggcgc
     3781 ggccggcgct tccggggcgg gcgggtccag gacgtccttg ccccacacca ggccccagga
     3841 ctcgacgagc cgccggacgc ccggtaggcc gtacgtctcg gcgaccttga tgaggtcgag
     3901 gcgacgtccg gcgacgcggg cgatgtatcg gtaccagatg taggccggga tgaccgcgat
     3961 ggcgaccagg ccctcggtgt cgtcggtgat ctcctcctcg gtgcggacgt cctgctggat
     4021 gccgagttcc ttgatcagcc ggttcaggtt ctgcgaccgg tagtgcttgc ggacctggaa
     4081 gacgccgaac tcgcgctcgc ggtacttctc gacgaacggg ccgggccgac gaagccgctg
     4141 cagctcggcg gccgccgcgt cgcccaggtc gagcggtccc atgcggtcgt cgccgcgacc
     4201 ggccttgaag ttctgtccgg ccagctccag gccgatcttg gcgacgccgc ccttggtctt
     4261 gtcgccgtcc ttgtagaggt agcgggcctg cttgcccgca tcgccgtcag cggcgtccgc
     4321 gccgttgagt gggcgcacgt cggtgccgtg gcccttgccc tcacaggagc aaccgggccg
     4381 gtcgcacgtc tcgctgacgg tgtagccgcc cgcggattcg accccggcgg cccaggctcc
     4441 ggcgagtgcg tcgcggaacg cggcctgggc gtccgggccg agcacctcgc gggtgaccca
     4501 gagcgtgtgc cagtgcaggt gccagccgga gccccagccg aaggtgtcct cgaaggcccg
     4561 ctcgtagccg atgatcccga agtcgtcgcg catcgtgcgc cagcggcggc cggacgagcc
     4621 gtacgcgccc ttccagccgt cgtgcaagac cgcgaccagg ccgtgccgca ttcccttgcg
     4681 gacggtgccg aacgccatgc gctcgaagtg gcgcaacgtg ttcgtgccaa ggtgcagccc
     4741 gtacccggcg tccgcgagac cgtcggcggc gagctgcacg ttcgagcccc gtacggccag
     4801 gatgcggctc atgcaccacg ggcaggtgtg gacgttgttg cagcggcacg tgttgcccca
     4861 cgtcgcctcg cccggcttcc acatcagctc ggccgtcccg gcagtgagcc gggtcccgca
     4921 gcccttgaac gcctcgttca gcgacaccgt ctggtgccgg tcccgccggg cgaaccgctc
     4981 gtcgcgcggg tcctcccgcc cggctgtcgc ggcaccctcg tttggggtag aacccgttcc
     5041 agttacagcg ctctgacctg cagtggacgg agattttccc ttactactaa agcccgcgtc
     5101 cggattaccc gctgtagtcg tgcttgctac gctgcgtgac tggtccgcaa tgagacgctt
     5161 tgcgcgcttt cggcaggcgt ccgagcagta gattttgggg cgcttcccgg ggatgtggac
     5221 gatcggggtg ccgcagtggc acttcggtcc ggcggggcgc ggtggtgtcg acgcgctgtt
     5281 ctctcgtacg ctcgtcacag agcaaacgtc ctcactcggc atgctgcgcc ggttcggggg
     5341 cggcgagccc gggaggccaa tcccgggctc gtgccatttc tgggtcctgt tgatcctggc
     5401 attggtgtgg ccgttcattg cccctgctcg ctcctgacgc gccgatagac gtccgatacg
     5461 cccggtgctg gtgggatttg ataggtcgga agaagccccg cccgggcctg ggcggggctt
     5521 cctgtgcgtc aggacctcct cgtcgtgagc ctcttcggcc tatggacgga gtgacctcgt
     5581 gatccgttac agccgcgcgc gctcgcgtag agcggtctca tcagttccac gaacggtcct
     5641 cttcgcagat cagggcgttg gggcggagtc tcaccaagga ctacgtctgc tggcgatttc
     5701 cgttacaccc cgggcggtgg ccggcgcaca cgcgcgcccg cgttgggcag tgcagaaagt
     5761 gcagaaacct aggcgctgat ggtccaggtc cacggttcgt cgtcggcggc ggcgcgggcg
     5821 gcggcgtcgg ccagggcgcg ggcgagaccg gctacggcgg gcttgatgcg ccggttgcgg
     5881 gcgaccttga gcagctagta tgcaggtcga cggatctttt ccgctgcata accctgcttc
     5941 ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt
     6001 tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata
     6061 ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg
     6121 gcggtgctca acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat
     6181 gagggcaagc ggatggctga tgaaaccaag ccaaccagga agggcagccc acctatcaag
     6241 gtgtactgcc ttccagacga acgaagagcg attgaggaaa aggcggcggc ggccggcatg
     6301 agcctgtcgg cctacctgct ggccgtcggc cagggctaca aaatcacggg cgtcgtggac
     6361 tatgagcacg tccgcgagct ggcccgcatc aatggcgacc tgggccgcct gggcggcctg
     6421 ctgaaactct ggctcaccga cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc
     6481 gccctgctgg cgaagatcga agagaagcag gacgagcttg gcaaggtcat gatgggcgtg
     6541 gtccgcccga gggcagagcc atgacttttt tagccgctaa aacggccggg gggtgcgcgt
     6601 gattgccaag cacgtcccca tgcgctccat caagaagagc gacttcgcgg agctggtgaa
     6661 gtacatcacc gacgagcaag gcaagaccga tccccgggga cctgcata
//
