LOCUS       V016272                 9470 bp    DNA     circular SYN 15-MAR-2020
DEFINITION  Exported.
ACCESSION   V016272
VERSION     V016272
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Kersten B, Leite Montalvao AP, Hoenicka H, Mueller NA, Vettori C,
            Paffetti D, Fladung M.
  TITLE     Resequencing of two transgenic early-flowering poplar lines
            confirmed integration of a single T-DNA copy in both lines without
            any unexpected additional integration of T-DNA vector fragments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Kersten B, Hoenicka H, Fladung M, Nilsson O.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2019) Genome Research, Thuenen Institute of Forest
            Genetics, Sieker Landstrasse 2, Grosshansdorf 22927, Germany
REFERENCE   3  (bases 1 to 9470)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan Pro v. v15.1
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-AUG-2019) Genome Research, Thuenen Institute of Forest Genetics,
            Sieker Landstrasse 2, Grosshansdorf 22927, Germany"
            The construction of the vector was described by Huang T. et al.
            (Science, 2005). The nucleotide sequence of the vector backbone is
            according to the binary GATEWAY destination vector pK2GW7 (Genbank
            accession JC487359). The vector  pK2GW7 was derived from plasmid
            pPZP200 (Genbank accession U10460; Hajdukiewicz P. et al., Plant
            Mol. Biol. 1994).
FEATURES             Location/Qualifiers
     source          1..9470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(651..1442)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(1476..1655)
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     regulatory      1773..2238
                     /note="HSP promoter from the soybean Gmhsp 17.6L heat-shock
                     protein gene"
                     /regulatory_class="promoter"
     protein_bind    2251..2275
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2276..2800
                     /gene="FT"
                     /label="Protein FLOWERING LOCUS T"
                     /note="Protein FLOWERING LOCUS T from Arabidopsis thaliana.
                     Accession#: Q9SXZ2"
     protein_bind    complement(2804..2828)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2838..3063
                     /label="CAMV 35S terminator (terminal 35S)"
                     /note="CAMV 35S terminator (terminal 35S)"
                     /regulatory_class="terminator"
     misc_feature    3143..3167
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4498..5124
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5561..6625
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6694..6888
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7232..7372
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7558..8146)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8393..9181)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
     1741 taattcgagc tcggtacccg gggatcctct agagaattcg atcgccaagc ttctcgagag
     1801 tcagcctttt aagagataga atttaaaata taatttgcgt aaaacattat taaaaataca
     1861 aatttataaa ttaagttcaa ctcatcctat ctcactcttt aaatacgatg tttacttatt
     1921 agactcatta ataaaaaaaa aaaaaatcat ttgtacaaag cccaccataa aggcaatttg
     1981 ggcctggtag accaatccta accaatgtct ggttaagatg gtccaatccc gaaacttcta
     2041 gttgcggttc gaagaagtcc agaatgtttc tgaaagtttc agaaaattct agttttgaga
     2101 ttttcagaag tacggcatga tgatgcataa caaggacttt ctcgaaagta ctatattgct
     2161 cctctacatc attttaaata ccccatgtgt cctttgaaga cacatcacag aaagaagtga
     2221 aggcatcgta tcaagcttac tagtgatatc acaagtttgt acaaaaaagc aggctatgtc
     2281 tataaatata agagaccctc ttatagtaag cagagttgtt ggagacgttc ttgatccgtt
     2341 taatagatca atcactctaa aggttactta tggccaaaga gaggtgacta atggcttgga
     2401 tctaaggcct tctcaggttc aaaacaagcc aagagttgag attggtggag aagacctcag
     2461 gaacttctat actttggtta tggtggatcc agatgttcca agtcctagca accctcacct
     2521 ccgagaatat ctccattggt tggtgactga tatccctgct acaactggaa caacctttgg
     2581 caatgagatt gtgtgttacg aaaatccaag tcccactgca ggaattcatc gtgtcgtgtt
     2641 tatattgttt cgacagcttg gcaggcaaac agtgtatgca ccagggtggc gccagaactt
     2701 caacactcgc gagtttgctg agatctacaa tctcggcctt cccgtggccg cagttttcta
     2761 caattgtcag agggagagtg gctgcggagg aagaagactt tagacccagc tttcttgtac
     2821 aaagtggtga tatcccgcgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca
     2881 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
     2941 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     3001 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
     3061 cctgcaggca tgcgacgtcg gagcttagct tgagcttgga tcagattgtc gtttcccgcc
     3121 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     3181 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     3241 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtacttta aagtacttta
     3301 aagtacttta aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
     3361 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
     3421 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
     3481 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
     3541 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
     3601 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
     3661 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
     3721 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
     3781 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
     3841 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
     3901 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
     3961 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
     4021 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
     4081 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
     4141 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
     4201 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
     4261 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga
     4321 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
     4381 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
     4441 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
     4501 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
     4561 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
     4621 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
     4681 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
     4741 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
     4801 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
     4861 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
     4921 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
     4981 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
     5041 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
     5101 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
     5161 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
     5221 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
     5281 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
     5341 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
     5401 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
     5461 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc
     5521 aatggcactg gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc
     5581 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     5641 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     5701 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     5761 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     5821 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     5881 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     5941 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
     6001 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
     6061 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
     6121 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
     6181 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
     6241 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
     6301 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
     6361 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
     6421 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
     6481 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
     6541 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
     6601 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
     6661 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc
     6721 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
     6781 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
     6841 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
     6901 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
     6961 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
     7021 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
     7081 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     7141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7201 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     7261 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7321 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     7381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7981 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8221 gcatgatata tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc
     8281 agacttgacc tgatagtttg gctgtgagca attatgtgct tagtgcatct aatcgcttga
     8341 gttaacgccg gcgaagcggc gtcggcttga acgaatttct agctagacat tatttgccga
     8401 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg
     8461 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct
     8521 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
     8581 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
     8641 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
     8701 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
     8761 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
     8821 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
     8881 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
     8941 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
     9001 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
     9061 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
     9121 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccccca
     9181 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
     9241 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     9301 gtaccccaaa aaaacatgtc ataacaagaa gccatgaaaa ccgccactgc gccgttacca
     9361 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgacg gcagttacgc tacttgcatt
     9421 acagcttacg aaccgaacga ggcttatgtc cactgggttc gtgcccgaat
//
