LOCUS       V013732                 9870 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013732
VERSION     V013732
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        734..764
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    772..788
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             808..981
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     CDS             1288..3936
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     CDS             4936..5727
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        5776..6221
                     /label="sacB promoter"
                     /note="sacB promoter and control region"
     CDS             6222..7640
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     oriT            complement(7997..8106)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        complement(8625..8715)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      9075..9663
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcaggattcc gtttcccgtc agctgaccgc tgatttcatc cagcaatggc aggctgctca
       61 cccggccgat cagatcacgg tgcgcgacct ggcggtcaac ccggtgccgc atctggatgc
      121 caacctgctg ggtggctgga tgaaacccga agaacagcgc agcgccgccg aattggatgc
      181 cctggcccga tccaacgaat tgaccgatga gttgctggca gccgacgtgc tggtgatggc
      241 cgcaccgatg tacaacttca ccatccccag caccctcaag gcctggttgg accatgtgct
      301 gcgcgccggc atcaccttca agtacacgcc tactggccca caaggcctgc tgaccggcaa
      361 gcgcgccatc gtcctgactg cccgcggcgg tatccatgcc ggtgccagca gcgaccacca
      421 ggaaccgtac ctgcgccagg tgatggcctt catcggtatt catgatgtcg acttcatcca
      481 tgccgaaggc ctgaacatga gcggcgaatt ccacgagaag ggcctcaacc aggccaaggc
      541 caacctggcg gcggtagcct gagattcact gaattcccaa ccctgacacc tgttgttgct
      601 ccttcgggtg tatctgcccg gcctgcatgg ccgggttttt ttgcccggca ttttgtgcag
      661 gccagtgcag cccctacatt cctcatggtt gaactcattt gccgcaaccc gctaaggtcg
      721 cggccttgat tcatttacac tttatgcttc cggctcgtat aatgtgtgga attgtgagcg
      781 gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt
      841 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      901 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      961 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
     1021 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
     1081 cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat
     1141 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
     1201 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgtcgg gatctgatcc
     1261 ggatttacta actggaagag gcactaaatg aacacgatta acatcgctaa gaacgacttc
     1321 tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt
     1381 ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc
     1441 cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag
     1501 cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa
     1561 gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg
     1621 gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat
     1681 acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc
     1741 ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac
     1801 aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc
     1861 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat
     1921 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc
     1981 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct
     2041 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc
     2101 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt
     2161 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt
     2221 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac
     2281 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac
     2341 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct
     2401 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc
     2461 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat
     2521 aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg
     2581 ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca
     2641 atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat
     2701 aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct
     2761 tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc
     2821 cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc
     2881 cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga
     2941 gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac
     3001 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat
     3061 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg
     3121 ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag
     3181 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg
     3241 gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat
     3301 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct
     3361 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa
     3421 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat
     3481 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc
     3541 ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca
     3601 cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt
     3661 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac
     3721 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact
     3781 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac
     3841 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac
     3901 ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaag agcaaggagg ctatcaatgc
     3961 acaccatctt cgtcatcgtc gcaccgattt tcgccttgat tctggtcggc tacttgtgcc
     4021 gcaaaaccaa caagctgggc gacaaggccg ccgccgaaat caacaagatg gtggtctggc
     4081 tgtgcctgcc ggcgttgctg ttcaaggtga cggccactgc cacctggagc gagatctggc
     4141 acccggggtt catcgcagcc ttcggcgcgg gggccctggc catgttcgta atcaccctgc
     4201 tgtggcgctt gaccagtggc catggcctgg tggcgtcgag catcgacggg ctcagtgccg
     4261 ggtatgccaa caccggctat ataggcatcc cgttgtgcct gctgctgttc ggccaggcgg
     4321 gcttgcagcc ggcgctgatc tcctctttga tcgtggtgtg cctggtgttt gccatttccc
     4381 tgacgctgat cgaggtcggg ctgcaggaag aacggcaaat cggccgcgcc atcgtggtgg
     4441 ttggcaaggc gctggcgaag aatccgttgg tgatctcgcc attggcgggg gcggcctggg
     4501 cggtgtctgg ctggggattg gcagagccgg tgatgcattt tctcgacatg ctggcgttgg
     4561 cgaccacgcc gtgcgcgctg gtgtcgttgg gcgccttcct ggccgaaaag cgcccaggtg
     4621 cgcaggccag cccatggccg ctggtggtca tgaaactggt ggggcagccg gcgctgacct
     4681 gggtgctggc gtacaaggtg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
     4741 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
     4801 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     4861 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga
     4921 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4981 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5041 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5101 gccctgaatg aactccaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5161 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5221 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5281 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5341 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5401 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5461 gcgcggatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5521 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5581 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5641 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5701 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgct agaggatcga
     5761 tcctttttaa cccatcacat atacctgccg ttcactatta tttagtgaaa tgagatatta
     5821 tgatattttc tgaattgtga ttaaaaaggc aactttatgc ccatgcaaca gaaactataa
     5881 aaaatacaga gaatgaaaag aaacagatag attttttagt tctttaggcc cgtagtctgc
     5941 aaatcctttt atgattttct atcaaacaaa agaggaaaat agaccagttg caatccaaac
     6001 gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg gtttgttact gataaagcag
     6061 gcaagaccta aaatgtgtaa agggcaaagt gtatactttg gcgtcacccc ttacatattt
     6121 taggtctttt tttattgtgc gtaactaact tgccatcttc aaacaggagg gctggaagaa
     6181 gcagaccgct aacacagtac ataaaaaagg agacatgaac gatgaacatc aaaaagtttg
     6241 caaaacaagc aacagtatta acctttacta ccgcactgct ggcaggaggc gcaactcaag
     6301 cgtttgcgaa agaaacgaac caaaagccat ataaggaaac atacggcatt tcccatatta
     6361 cacgccatga tatgctgcaa atccctgaac agcaaaaaaa tgaaaaatat caagtttctg
     6421 aatttgattc gtccacaatt aaaaatatct cttctgcaaa aggcctggac gtttgggaca
     6481 gctggccatt acaaaacgct gacggcactg tcgcaaacta tcacggctac cacatcgtct
     6541 ttgcattagc cggagatcct aaaaatgcgg atgacacatc gatttacatg ttctatcaaa
     6601 aagtcggcga aacttctatt gacagctgga aaaacgctgg ccgcgtcttt aaagacagcg
     6661 acaaattcga tgcaaatgat tctatcctaa aagaccaaac acaagaatgg tcaggttcag
     6721 ccacatttac atctgacgga aaaatccgtt tattctacac tgatttctcc ggtaaacatt
     6781 acggcaaaca aacactgaca actgcacaag ttaacgtatc agcatcagac agctctttga
     6841 acatcaacgg tgtagaggat tataaatcaa tctttgacgg tgacggaaaa acgtatcaaa
     6901 atgtacagca gttcatcgat gaaggcaact acagctcagg cgacaaccat acgctgagag
     6961 atcctcacta cgtagaagat aaaggccaca aatacttagt atttgaagca aacactggaa
     7021 ctgaagatgg ctaccaaggc gaagaatctt tatttaacaa agcatactat ggcaaaagca
     7081 catcattctt ccgtcaagaa agtcaaaaac ttctgcaaag cgataaaaaa cgcacggctg
     7141 agttagcaaa cggcgctctc ggtatgattg agctaaacga tgattacaca ctgaaaaaag
     7201 tgatgaaacc gctgattgca tctaacacag taacagatga aattgaacgc gcgaacgtct
     7261 ttaaaatgaa cggcaaatgg tacctgttca ctgactcccg cggatcaaaa atgacgattg
     7321 acggcattac gtctaacgat atttacatgc ttggttatgt ttctaattct ttaactggcc
     7381 catacaagcc gctgaacaaa actggccttg tgttaaaaat ggatcttgat cctaacgatg
     7441 taacctttac ttactcacac ttcgctgtac ctcaagcgaa aggaaacaat gtcgtgatta
     7501 caagctatat gacaaacaga ggattctacg cagacaaaca atcaacgttt gcgccgagct
     7561 tcctgctgaa catcaaaggc aagaaaacat ctgttgtcaa agacagcatc cttgaacaag
     7621 gacaattaac agttaacaaa taaaaacgca aaagaaaatg ccgatgggta ccgagcgaaa
     7681 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     7741 atgaaaggtt gggcttcgga atcgttttcc gggacgccct cgcggacgtg ctcatagtcc
     7801 acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga caggctcatg
     7861 ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca gtacaccttg
     7921 ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct
     7981 gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg
     8041 tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc
     8101 ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat
     8161 cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag
     8221 cagggttatg cagcggaaaa gcgctgcttc cctgctgttt tgtggaatat ctaccgactg
     8281 gaaacaggca aatgcaggaa attactgaac tgaggggaca ggcgagagac gatgccaaag
     8341 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     8401 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca
     8461 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt
     8521 cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg
     8581 tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctcc tgaaaatctc
     8641 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     8701 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
     8761 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg
     8821 gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
     8881 aggtgctcca gtggcttctg tttctatcag ggctggatga tcctccagcg cggggatctc
     8941 atgctggagt tcttcgccca ccccaaaagg atctaggtga agatcctttt tgataatctc
     9001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9181 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9841 gaagagcgcc caatacgcaa accgcctctc
//
